Toxiciteit van combinaties van remmers van de kinaseroute voor normale menselijke cellen in een driedimensionale cultuur
Resistance to single-agent chemotherapy and molecularly targeted drugs prevents sustained efficacy of treatments. To address this challenge, combination drug treatments have been used to improve outcomes for patients. Potential toxicity of combination treatments is a major concern, however, and has led to the failure of several clinical trials in different cancers. The use of cell-based models of normal tissues in preclinical studies enables testing and identifying toxic effects of drug combinations and facilitates an informed decision-making process for advancing the treatments to animal models and clinical trials. Recently, we established that combinations of molecular inhibitors of mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase-protein kinase B (PI3K/Akt) pathways effectively and synergistically inhibit growth of BRAFmut and KRASmut colorectal tumor spheroids by blocking feedback signaling of downstream kinase pathways. These pathways are important for cell proliferation, however, and their simultaneous inhibition may cause toxicity to normal cells. We used a cellular spheroid model to study toxicities of drug combinations to human bone marrow and colon. Our results indicated that MAPK and PI3K/Akt inhibitors used simultaneously were only moderately toxic to bone marrow cells but significantly more toxic to colon cells. Our molecular analysis of proliferative cell activities and housekeeping proteins further corroborated these results. Overall, our approach to identify toxic effects of combinations of cancer drugs to normal cells in three-dimensional cultures will facilitate more informed treatment selections for subsequent animal studies.
Gehumaniseerd kweekmedium voor het genereren van klinische kwaliteit van erytroïde cellen uit navelstrengbloed CD34 + cellen
Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost.
Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated.
Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99±17×103-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium.
Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation.
Human Testosterone ELISA Kit |
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| EH4850 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens ;Sensitivity: 0.188 ng/ml |
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Human SPOCK2(Testican-2) ELISA Kit |
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| EH2271 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human TEX101(testis expressed 101) ELISA Kit |
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| EH12903 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens ;Sensitivity: 0.188 ng/ml |
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Human F-TESTO(Free Testoterone) ELISA Kit |
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| EH3092 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human ApoB48 ELISA Kit |
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| EH4928 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 ng/ml |
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Human APOE(Apolipoprotein E ) ELISA Kit |
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| EH0549 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.75 ng/ml |
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Human APOM(Apolipoprotein M) ELISA Kit |
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| EH2134 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human APOD(Apolipoprotein D) ELISA Kit |
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| EH2135 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.375 ng/ml |
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Human ApoF(Apolipoprotein F) ELISA Kit |
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| EH2657 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human APOL1(Apolipoprotein L1) ELISA Kit |
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| EH1181 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml |
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Human ApoB100(Apolipoprotein B100) ELISA Kit |
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| EH0074 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5 ng/ml |
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Human APOA1(Apolipoprotein A-I ) ELISA Kit |
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| EH0545 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human APOA5(Apolipoprotein A-V) ELISA Kit |
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| EH1952 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human APOA4(Apolipoprotein A-IV) ELISA Kit |
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| EH0618 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 2.813 ng/ml |
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Human APOB(Apolipoprotein B-100) ELISA Kit |
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| EH0620 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875 ng/ml |
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Human APOA2(Apolipoprotein A-II) ELISA Kit |
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| EH0633 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5 ng/ml |
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Rat ApoC3(Apolipoprotein C3) ELISA Kit |
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| ER0741 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 3.75 ng/ml |
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Monkey ApoC3(Apolipoprotein C3) ELISA Kit |
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| EMK0008 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.938 ng/ml |
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Rabbit ApoC1(Apolipoprotein C1) ELISA Kit |
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| ERB0012 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 0.094 ng/ml |
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Accu-Tell COVID-19 IgG/IgM Rapid Test |
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| GEN-B352-20tests | Accu test | 20 tests | EUR 283.2 |
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Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
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Mouse APO-C1(Apolipoprotein C1) ELISA Kit |
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| EM0844 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 37.5 ng/ml |
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Mouse APO-C4(Apolipoprotein C4) ELISA Kit |
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| EM0845 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml |
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Human PDHA2(Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial) ELISA Kit |
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| EH1149 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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T(Testosterone) ELISA Kit |
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| EU0400 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: General;Sensitivity: 0.188 ng/ml |
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Mouse Testosterone ELISA Kit |
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| EM1850 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml |
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Rat T(Testosterone) ELISA Kit |
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| ER1462 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml |
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Human BAX(Apoptosis regulator BAX) ELISA Kit |
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| EH0669 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml |
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Human APOH(Beta-2-glycoprotein 1 ) ELISA Kit |
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| EH0531 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 28.125pg/ml |
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Bovine T(Testosterone) ELISA Kit |
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| EB0049 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Cattle;Sensitivity: 0.188 ng/ml |
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Human APOA1BP(Apolipoprotein A-I-binding protein) ELISA Kit |
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| EH1180 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human FASL(Factor Related Apoptosis Ligand) ELISA Kit |
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| EH0127 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human SIVA1(Apoptosis regulatory protein Siva) ELISA Kit |
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| EH2267 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.9pg/ml |
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Human Apo A1-Ab(Anti-Apolipoprotein A1 Antibody) ELISA Kit |
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| EH2655 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Sandwich ELISA, Double Antigen ;Reacts with: Homo sapiens;Sensitivity: 1.875 ng/ml |
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Human EGF ELISA kit (Quick Test) |
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| E22-HC126QT.48 | EnoGene | 48T | EUR 495 |
Human EGF ELISA kit (Quick Test) |
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| E22-HC126QT.96 | EnoGene | 96T | EUR 595 |
Human PAWR(PRKC apoptosis WT1 regulator protein) ELISA Kit |
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| EH10908 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human FAIM3(Fas Apoptotic Inhibitory Molecule 3) ELISA Kit |
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| EH3035 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human VEGF ELISA kit (Quick Test) |
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| E22-HC108QT.48 | EnoGene | 48T | EUR 495 |
Human VEGF ELISA kit (Quick Test) |
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| E22-HC108QT.96 | EnoGene | 96T | EUR 595 |
2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) |
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| GEN-402-25tests | All test | 25 tests | EUR 292.8 |
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Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
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Human PUMA(P53 Upregulated Modulator of Apoptosis) ELISA Kit |
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| EH0250 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human CD94 ELISA Kit |
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| EH7228 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml |
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Human NAIF1(Nuclear apoptosis-inducing factor 1) ELISA Kit |
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| EH1223 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human TIGAR ELISA Kit |
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| EH4927 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human CD226 ELISA Kit |
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| EH7177 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml |
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Human EPAC2 ELISA Kit |
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| EH8258 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human APAF1(Apoptosis Protease Activating Factor 1) ELISA Kit |
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| EH2654 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human TRIM14 ELISA Kit |
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| EH13190 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human Spexin ELISA Kit |
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| EH4349 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 4.688pg/ml |
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Human Irisin ELISA Kit |
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| EH4702 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human PRSS36 ELISA Kit |
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| EH4924 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human SHARPIN ELISA Kit |
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| EH12274 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human SERPIND1 ELISA Kit |
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| EH12199 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human Thrombin ELISA Kit |
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| EH4141 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human Asprosin ELISA Kit |
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| EH4176 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human KITLG(Kit ligand ) ELISA Kit |
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| EH0514 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human ASK-1(Apoptosis Signal Regulating Kinase 1) ELISA Kit |
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| EH2670 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human Neopterin ELISA Kit |
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| EH3413 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human CFLAR(CASP8 and FADD-like apoptosis regulator) ELISA Kit |
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| EH1762 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.9pg/ml |
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Human Calprotectin ELISA Kit |
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| EH4140 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375 ng/ml |
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Human Pre-S1 ELISA Kit |
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| EH4111 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Qualitative ;Reacts with: Homo sapiens;Sensitivity: Qualitative |
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Human CRN(Corin) ELISA Kit |
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| EH0097 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 45pg/ml |
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Human FUR(Furin) ELISA Kit |
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| EH0143 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human REN(Renin) ELISA Kit |
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| EH0268 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human KL(Klotho) ELISA Kit |
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| EH2184 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 4.688pg/ml |
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Human PP(Pepsin) ELISA Kit |
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| EH3629 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5pg/ml |
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Human EZR(Ezrin) ELISA Kit |
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| EH1471 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human TTN(Titin) ELISA Kit |
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| EH1877 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human Rbx2(RNF7) ELISA Kit |
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| EH4196 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human Activin A ELISA Kit |
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| EH0028 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5pg/ml |
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Human LN(Laminin) ELISA Kit |
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| EH0214 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human LEP(Leptin) ELISA Kit |
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| EH0216 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human MK(Midkine) ELISA Kit |
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| EH0229 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human NDN(Necdin) ELISA Kit |
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| EH0866 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human NOG(Noggin) ELISA Kit |
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| EH1051 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human NES(Nestin) ELISA Kit |
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| EH2140 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human AFM(Afamin) ELISA Kit |
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| EH2493 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 1.875 ng/ml |
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Human Des(Desmin) ELISA Kit |
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| EH2944 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human AGRN(Agrin) ELISA Kit |
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| EH1477 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human NDP(Norrin) ELISA Kit |
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| EH1664 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human MEFV(Pyrin) ELISA Kit |
|||
| EH2102 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human DCN(Decorin) ELISA Kit |
|||
| EH0111 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.75 ng/ml |
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Human LUM(Lumican) ELISA Kit |
|||
| EH0220 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
|||
Human INS(Insulin) ELISA Kit |
|||
| EH0374 | FN Test | 96T | EUR 571.5 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
|||
Human FE(Ferritin) ELISA Kit |
|||
| EH0382 | FN Test | 96T | EUR 571.5 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
|||
Human HCRT(Orexin) ELISA Kit |
|||
| EH1045 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
|||
Human RELN(Reelin) ELISA Kit |
|||
| EH2121 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
|||
Human APLN(Apelin) ELISA Kit |
|||
| EH2174 | FN Test | 96T | EUR 571.5 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5pg/ml |
|||
Human OGN(Mimecan) ELISA Kit |
|||
| EH2412 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
|||
Human MTL(Motilin) ELISA Kit |
|||
| EH3393 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
|||
Human ELN(Elastin) ELISA Kit |
|||
| EH1505 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.281 ng/ml |
|||
Human GC(Glucagon) ELISA Kit |
|||
| EH1944 | FN Test | 96T | EUR 571.5 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
|||
Human ACR(Acrosin) ELISA Kit |
|||
| EH2014 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 3.75mIU/ml |
|||
Human TRY(Trypsin) ELISA Kit |
|||
| EH3915 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
|||
Human OSX(Osterix) ELISA Kit |
|||
| EH4043 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human Es(Estrogen) ELISA Kit |
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| EH4184 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human OT(Oxytocin) ELISA Kit |
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| EH4212 | FN Test | 96T | EUR 571.5 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human Aldolase B ELISA Kit |
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| EH4806 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.281 ng/ml |
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Human ENG(Endoglin) ELISA Kit |
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| EH0071 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human GHRL(Ghrelin) ELISA Kit |
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| EH0355 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 28.125pg/ml |
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Human COR(Cortisol) ELISA Kit |
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| EH0641 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.234 ng/ml |
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Human CAT(Catalase) ELISA Kit |
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| EH0643 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human STH(Saitohin) ELISA Kit |
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| EH0832 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human GSN(Gelsolin) ELISA Kit |
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| EH0875 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 3.75 ng/ml |
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Human VIM(Vimentin) ELISA Kit |
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| EH1166 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 4.688 ng/ml |
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Human ASPN(Asporin) ELISA Kit |
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| EH1174 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human SCT(Secretin) ELISA Kit |
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| EH1210 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human BGN(Biglycan) ELISA Kit |
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| EH1218 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human ENHO(Adropin) ELISA Kit |
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| EH2290 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human CNX(Calnexin) ELISA Kit |
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| EH2856 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.375 ng/ml |
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Human CPP(Copeptin) ELISA Kit |
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| EH2880 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human MT(Melatonin) ELISA Kit |
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| EH3344 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 4.688pg/ml |
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Human Pax(Paxillin) ELISA Kit |
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| EH3511 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human PDCN(Podocin) ELISA Kit |
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| EH3529 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human GAST(Gastrin) ELISA Kit |
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| EH1397 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188pg/ml |
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Human PRX(Periaxin) ELISA Kit |
|||
| EH1492 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human OPTC(Opticin) ELISA Kit |
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| EH1604 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.9pg/ml |
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Human PLEC(Plectin) ELISA Kit |
|||
| EH1770 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human FSCN1(Fascin) ELISA Kit |
|||
| EH1915 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml |
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Human VCL(Vinculin) ELISA Kit |
|||
| EH1957 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human TNC(Tenascin) ELISA Kit |
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| EH2027 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human OB(Obestatin) ELISA Kit |
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| EH2063 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human CMA1(Chymase) ELISA Kit |
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| EH2077 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human TPS(Tryptase) ELISA Kit |
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| EH3906 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5pg/ml |
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Human S100 Protein ELISA Kit |
|||
| EH4865 | FN Test | 96T | EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human Histone-H3 ELISA Kit |
|||
| EH9123 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml |
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Human CLU(Clusterin) ELISA Kit |
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| EH0004 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 1.875 ng/ml |
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Human CHEM(Chemerin) ELISA Kit |
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| EH0092 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human ES(Endostatin) ELISA Kit |
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| EH0119 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
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Human CCL11(Eotaxin) ELISA Kit |
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| EH0121 | FN Test | 96T | EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human PRL(Prolactin) ELISA Kit |
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| EH0259 | FN Test | 96T | EUR 571.5 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human RETN(Resistin) ELISA Kit |
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| EH0269 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human Surv(Survivin) ELISA Kit |
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| EH0277 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human Myo(Myoglobin) ELISA Kit |
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| EH0347 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human CT(Calcitonin) ELISA Kit |
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| EH0377 | FN Test | 96T | EUR 571.5 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human PI(Proinsulin) ELISA Kit |
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| EH0388 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human GRN(Granulins ) ELISA Kit |
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| EH0520 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human NRN1(Neuritin) ELISA Kit |
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| EH0944 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human NPHS1(Nephrin) ELISA Kit |
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| EH1086 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 1.875pg/ml |
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Human CFP(Properdin) ELISA Kit |
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| EH1217 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human HRNR(Hornerin) ELISA Kit |
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| EH1240 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human RNLS(Renalase) ELISA Kit |
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| EH1250 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml |
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Human MYOG(Myogenin) ELISA Kit |
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| EH1256 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.375 ng/ml |
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Human STOM(Stomatin)ELISA Kit |
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| EH12686 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human UCN(Urocortin) ELISA Kit |
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| EH2157 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human UTRN(Utrophin) ELISA Kit |
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| EH2376 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml |
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Human HDLBP(Vigilin) ELISA Kit |
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| EH2505 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human his(Histamine) ELISA Kit |
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| EH2552 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Human Amylase Alpha ELISA Kit |
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| EH2652 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.4mIU/ml |
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Human ARO(Aromatase) ELISA Kit |
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| EH2665 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Moleculaire cytogenetische analyse en het opzetten van een celkweek bij de vissoort Hollandichthys multifasciatus (Eigenmann & amp; Norris, 1900) (Characiformes, Characidae)
Hollandichthys is a fish genus of the family Characidae that was until recently considered to be monotypic, with cytogenetic, morphological, and molecular data being restricted to a few local populations. In the present study, the karyotype of a population of Hollandichthys multifasciatus was analyzed using classical and molecular cytogenetic approaches for the investigation of potential markers that could provide new perspectives on the cytotaxonomy. H. multifasciatus presented a diploid number of 2n=50 chromosomes and a karyotype formula of 8m+10sm+32st. A single pair of chromosomes presented Ag-NORs signals, which coincided with the 18S rDNA sites visualized by FISH, whilst the 5S rDNA sequences were mapped in two chromosome pairs. The distribution of the U snRNA genes was mapped on the Hollandichthys chromosomes for the first time, with the probes revealing the presence of the U1 snDNA on the chromosomes of pair 20, U2 on pairs 6 and 19, U4 on pair 16, and U6 on the chromosomes of pair 11. The results of the present study indicated karyotypic differences in comparison with the other populations of H. multifasciatus studied previously, reinforcing the need for further research to identify isolated populations or the potential existence of cryptic Hollandichthys species.
• The effects of salt stress and adaptation on salicylic acid (SA) content and on antioxidant and lipoxygenase (LOX) enzyme activities were studied in tomato (Lycopersicon esculentum cv. Pera) cells. • NaCl-adapted cells were obtained from calli adapted to 100 mm NaCl by successive subcultures in medium supplemented with 100 mm NaCl. Salt stress treatments consisted of the addition of 100 mm NaCl to cells. • Adapted cells contained a lower concentration of SA than unadapted cells. The lower manganese-containing superoxide dismutase (Mn-SOD) and LOX activities as well as the higher glutathione reductase (GR) and ascorbate peroxidase (APX) activities in adapted cells than in unadapted cells could be correlated with the development of salt adaptation. Salt stress increased APX and LOX activities as well as lipid peroxidation in unadapted cells and increased Mn-SOD activity in both types of cells. Application of 200 µm SA + 100 mm NaCl inhibited APX activity in both unadapted and adapted cells, induced the Mn-SOD in adapted cells and increased lipid peroxidation in unadapted cells. • Our data indicate that adaptation of tomato cells to NaCl results in a higher tolerance to NaCl-induced oxidative stress and suggest a role for SA in this response.