Toxiciteit van combinaties van remmers van de kinaseroute voor normale menselijke cellen in een driedimensionale cultuur
Resistance to single-agent chemotherapy and molecularly targeted drugs prevents sustained efficacy of treatments. To address this challenge, combination drug treatments have been used to improve outcomes for patients. Potential toxicity of combination treatments is a major concern, however, and has led to the failure of several clinical trials in different cancers. The use of cell-based models of normal tissues in preclinical studies enables testing and identifying toxic effects of drug combinations and facilitates an informed decision-making process for advancing the treatments to animal models and clinical trials. Recently, we established that combinations of molecular inhibitors of mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase-protein kinase B (PI3K/Akt) pathways effectively and synergistically inhibit growth of BRAFmut and KRASmut colorectal tumor spheroids by blocking feedback signaling of downstream kinase pathways. These pathways are important for cell proliferation, however, and their simultaneous inhibition may cause toxicity to normal cells. We used a cellular spheroid model to study toxicities of drug combinations to human bone marrow and colon. Our results indicated that MAPK and PI3K/Akt inhibitors used simultaneously were only moderately toxic to bone marrow cells but significantly more toxic to colon cells. Our molecular analysis of proliferative cell activities and housekeeping proteins further corroborated these results. Overall, our approach to identify toxic effects of combinations of cancer drugs to normal cells in three-dimensional cultures will facilitate more informed treatment selections for subsequent animal studies.
Gehumaniseerd kweekmedium voor het genereren van klinische kwaliteit van erytroïde cellen uit navelstrengbloed CD34 + cellen
Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost.
Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated.
Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99±17×103-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium.
Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation.
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OKSA11303 | Aviva Systems Biology | 10 Tests | EUR 780 |
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CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110 |
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CASLV100PA-KIT | SBI | 1 Kit | EUR 1132 |
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Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood |
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TS00301s-30 | Arthus | 30 tests | EUR 504 |
Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood |
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TS00401s-30 | Arthus | 30 tests | EUR 553 |
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CAS700A-KIT | SBI | 10 rxn | EUR 1132 |
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CAS720A-KIT | SBI | 10 rxn | EUR 1132 |
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CAS740A-KIT | SBI | 10 rxn | EUR 1132 |
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CAS510A-KIT | SBI | 1 Kit | EUR 805 |
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CASLV205PA-KIT | SBI | 1 Kit | EUR 1132 |
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CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
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CASLV225PA-KIT | SBI | 1 Kit | EUR 1132 |
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CAS750A-KIT | SBI | 10 rxn | EUR 1132 |
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CAS770A-KIT | SBI | 10 rxn | EUR 1132 |
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CAS790A-KIT | SBI | 10 rxn | EUR 1132 |
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CAS9LIG-KIT | SBI | 1 Kit | EUR 153 |
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PIN320A-KIT | SBI | 1 Kit | EUR 4941 |
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PIN340iPS-KIT | SBI | 1 Kit | EUR 4941 |
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115 | Biobase | 96T/Box | Ask for price |
Description: ELISA based test for quantitative detection of ANA Antinuclear Ab IgG |
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abx092012-25tests | Abbexa | 25 tests | EUR 425 |
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abx092079-20tests | Abbexa | 20 tests | EUR 258 |
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abx092080-20tests | Abbexa | 20 tests | EUR 258 |
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abx092081-20tests | Abbexa | 20 tests | EUR 258 |
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abx092082-20tests | Abbexa | 20 tests | EUR 258 |
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abx092084-20tests | Abbexa | 20 tests | EUR 230 |
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abx092090-20tests | Abbexa | 20 tests | EUR 230 |
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abx092091-20tests | Abbexa | 20 tests | EUR 342 |
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abx092094-20tests | Abbexa | 20 tests | EUR 272 |
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abx092096-20tests | Abbexa | 20 tests | EUR 398 |
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abx092097-20tests | Abbexa | 20 tests | EUR 217 |
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abx092100-20tests | Abbexa | 20 tests | EUR 342 |
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abx092101-20tests | Abbexa | 20 tests | EUR 258 |
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abx092040-50tests | Abbexa | 50 tests | EUR 272 |
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abx092042-50tests | Abbexa | 50 tests | EUR 300 |
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abx092043-50tests | Abbexa | 50 tests | EUR 342 |
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abx092045-50tests | Abbexa | 50 tests | EUR 356 |
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abx092046-50tests | Abbexa | 50 tests | EUR 537 |
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abx092047-50tests | Abbexa | 50 tests | EUR 551 |
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abx092048-50tests | Abbexa | 50 tests | EUR 467 |
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abx092051-50tests | Abbexa | 50 tests | EUR 411 |
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abx092052-50tests | Abbexa | 50 tests | EUR 467 |
![]() Deoxynivalenol Rapid Test Kit (Colloidal gold) |
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abx092053-50tests | Abbexa | 50 tests | EUR 467 |
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abx092054-50tests | Abbexa | 50 tests | EUR 398 |
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abx092056-50tests | Abbexa | 50 tests | EUR 425 |
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abx092070-40tests | Abbexa | 40 tests | EUR 356 |
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abx092071-40tests | Abbexa | 40 tests | EUR 356 |
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abx092074-50tests | Abbexa | 50 tests | EUR 321 |
![]() Anigen Rapid FIV Ab Test Kit |
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RB22-01-DD | Bionote | 10 tests/Kit | EUR 129.8 |
Description: Please check the datasheet of Anigen Rapid FIV Ab Test Kit before using the test. |
![]() Rapid Bovine Brucella Ab Test Kit |
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RB23-01-DD | Bionote | 1 kit/10 tests | EUR 151.79 |
Description: Please check the datasheet of Rapid Bovine Brucella Ab Test Kit before using the test. |
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RB23-02-DD | Bionote | 1 box | EUR 128.4 |
Description: Please check the datasheet of Rapid Bovine TB Ab Test Kit before using the test. |
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RB28-02-DD | Bionote | 1 box | EUR 135.4 |
Description: Please check the datasheet of Rapid FMD NSP Ab Test Kit before using the test. |
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RC12-04 | Bionote | 10 tests/kit | EUR 170.4 |
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RG11-03 | Bionote | 1 box | EUR 138.2 |
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RG14-03 | Bionote | 1 box | EUR 240.85 |
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![]() Ag Rapid AIV Ag Test Kit |
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RG15-01 | Bionote | 1 box/30 tests | EUR 267 |
Description: Please check the datasheet of Ag Rapid AIV Ag Test Kit before using the test. |
![]() Ag Rapid H5AIV Ag test kit |
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RG15-05 | Bionote | 1 box | EUR 463 |
Description: Please check the datasheet of Ag Rapid H5AIV Ag test kit before using the test. |
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OKSA11304 | Aviva Systems Biology | 10 Tests | EUR 924 |
Description: Description of target: RAT MONOCLONAL ANTIBODY ISOTYPING TEST KIT;Species reactivity: Rat;Application: IS;Assay info: ;Sensitivity: |
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GE620A-KIT | SBI | 1 kit | EUR 2132 |
![]() AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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GE624A-KIT | SBI | 1 kit | EUR 2132 |
![]() AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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GE622A-KIT | SBI | 1 kit | EUR 2132 |
![]() Clearview Chlamydia MF test |
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CV504524 | Bionote | 1 box | EUR 162 |
Description: Please check the datasheet of Clearview Chlamydia MF test before using the test. |
![]() IBD rapid test strip |
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RG15-04 | Bionote | 10 boxes | EUR 148 |
Description: Please check the datasheet of IBD rapid test strip before using the test. |
![]() Rabies rapid test strip |
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RG18-01 | Bionote | 10 boxes | EUR 151.92 |
Description: Please check the datasheet of Rabies rapid test strip before using the test. |
![]() Basic Cytotoxicity Test Kits |
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CT17001 | Neuromics | 125 Tests | EUR 409 |
![]() Basic Cytotoxicity Test Kits |
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CT17002 | Neuromics | 250 Tests | EUR 650 |
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CT17003 | Neuromics | 125 Tests | EUR 513 |
![]() Total Cytotoxicity Test Kits |
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CT17004 | Neuromics | 250 Tests | EUR 813 |
![]() HCG Anti-Human Chorionic Gonadotrophin Ab IgA ELISA test |
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128 | Biobase | 96T/Box | Ask for price |
Description: ELISA based test for quantitative detection of HCG Anti-Human Chorionic Gonadotrophin Ab IgA |
![]() HCG Anti-Human Chorionic Gonadotrophin Ab IgM ELISA test |
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129 | Biobase | 96T/Box | Ask for price |
Description: ELISA based test for quantitative detection of HCG Anti-Human Chorionic Gonadotrophin Ab IgM |
![]() HCG Anti-Human Chorionic Gonadotrophin Ab IgG ELISA test |
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130 | Biobase | 96T/Box | Ask for price |
Description: ELISA based test for quantitative detection of HCG Anti-Human Chorionic Gonadotrophin Ab IgG |
Moleculaire cytogenetische analyse en het opzetten van een celkweek bij de vissoort Hollandichthys multifasciatus (Eigenmann & amp; Norris, 1900) (Characiformes, Characidae)
Hollandichthys is a fish genus of the family Characidae that was until recently considered to be monotypic, with cytogenetic, morphological, and molecular data being restricted to a few local populations. In the present study, the karyotype of a population of Hollandichthys multifasciatus was analyzed using classical and molecular cytogenetic approaches for the investigation of potential markers that could provide new perspectives on the cytotaxonomy. H. multifasciatus presented a diploid number of 2n=50 chromosomes and a karyotype formula of 8m+10sm+32st. A single pair of chromosomes presented Ag-NORs signals, which coincided with the 18S rDNA sites visualized by FISH, whilst the 5S rDNA sequences were mapped in two chromosome pairs. The distribution of the U snRNA genes was mapped on the Hollandichthys chromosomes for the first time, with the probes revealing the presence of the U1 snDNA on the chromosomes of pair 20, U2 on pairs 6 and 19, U4 on pair 16, and U6 on the chromosomes of pair 11. The results of the present study indicated karyotypic differences in comparison with the other populations of H. multifasciatus studied previously, reinforcing the need for further research to identify isolated populations or the potential existence of cryptic Hollandichthys species.
• The effects of salt stress and adaptation on salicylic acid (SA) content and on antioxidant and lipoxygenase (LOX) enzyme activities were studied in tomato (Lycopersicon esculentum cv. Pera) cells. • NaCl-adapted cells were obtained from calli adapted to 100 mm NaCl by successive subcultures in medium supplemented with 100 mm NaCl. Salt stress treatments consisted of the addition of 100 mm NaCl to cells. • Adapted cells contained a lower concentration of SA than unadapted cells. The lower manganese-containing superoxide dismutase (Mn-SOD) and LOX activities as well as the higher glutathione reductase (GR) and ascorbate peroxidase (APX) activities in adapted cells than in unadapted cells could be correlated with the development of salt adaptation. Salt stress increased APX and LOX activities as well as lipid peroxidation in unadapted cells and increased Mn-SOD activity in both types of cells. Application of 200 µm SA + 100 mm NaCl inhibited APX activity in both unadapted and adapted cells, induced the Mn-SOD in adapted cells and increased lipid peroxidation in unadapted cells. • Our data indicate that adaptation of tomato cells to NaCl results in a higher tolerance to NaCl-induced oxidative stress and suggest a role for SA in this response.