fondationvocation

Gehumaniseerd kweekmedium voor het genereren van klinische kwaliteit van

Toxiciteit van combinaties van remmers van de kinaseroute voor normale menselijke cellen in een driedimensionale cultuur

Resistance to single-agent chemotherapy and molecularly targeted drugs prevents sustained efficacy of treatments. To address this challenge, combination drug treatments have been used to improve outcomes for patients. Potential toxicity of combination treatments is a major concern, however, and has led to the failure of several clinical trials in different cancers. The use of cell-based models of normal tissues in preclinical studies enables testing and identifying toxic effects of drug combinations and facilitates an informed decision-making process for advancing the treatments to animal models and clinical trials. Recently, we established that combinations of molecular inhibitors of mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase-protein kinase B (PI3K/Akt) pathways effectively and synergistically inhibit growth of BRAFmut and KRASmut colorectal tumor spheroids by blocking feedback signaling of downstream kinase pathways. These pathways are important for cell proliferation, however, and their simultaneous inhibition may cause toxicity to normal cells. We used a cellular spheroid model to study toxicities of drug combinations to human bone marrow and colon. Our results indicated that MAPK and PI3K/Akt inhibitors used simultaneously were only moderately toxic to bone marrow cells but significantly more toxic to colon cells. Our molecular analysis of proliferative cell activities and housekeeping proteins further corroborated these results. Overall, our approach to identify toxic effects of combinations of cancer drugs to normal cells in three-dimensional cultures will facilitate more informed treatment selections for subsequent animal studies.

Gehumaniseerd kweekmedium voor het genereren van klinische kwaliteit van erytroïde cellen uit navelstrengbloed CD34 cellen

 

 

Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost.
 Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated.
Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99±17×103-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium.
 Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation.

fondationvocation

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

FERR ( Ferritin) ELISA test

7 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FERR ( Ferritin)

Syphilis Antibody ELISA test

100 96T/Box Ask for price
Description: ELISA based test for quantitative detection of Syphilis Antibody

TSH (Thyrotrophin) ELISA test

14 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TSH (Thyrotrophin)

PRL (Prolactin) ELISA test

4 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PRL (Prolactin)

E2 (Estradiol) ELISA test

5 96T/Box Ask for price
Description: ELISA based test for quantitative detection of E2 (Estradiol)

PROG (Progesterone) ELISA test

20 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PROG (Progesterone)

TESTO (Testosterone) ELISA test

19 96T/Box Ask for price
Description: ELISA based test for quantitative detection of O (osterone)

T3 (Triiodothyronine) ELISA test

15 96T/Box Ask for price
Description: ELISA based test for quantitative detection of T3 (Triiodothyronine)

T4 (Thyroxine) ELISA test

16 96T/Box Ask for price
Description: ELISA based test for quantitative detection of T4 (Thyroxine)

CA72-4 ELISA test

24 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA72-4

HBcAb Sandwich ELISA test

95 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HBcAb Sandwich

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-20tests 20 tests
EUR 283.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests 40 tests
EUR 385.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Melamine Rapid Test Kit

abx092011-50tests 50 tests
EUR 444

NDV rapid test kit

RG15-03 1 box
EUR 166.86
Description: Please check the datasheet of NDV rapid test kit before using the test.

Staphyloslide Latex Test Kit

240915 PK100
EUR 272.46

Hach Test Kit CYANIDE

LCK315 EACH
EUR 144.28

Hach Test Kit FLUORIDE

LCK323 EACH
EUR 136.62

Hach Test Kit COPPER

LCK329 EACH
EUR 178.75

Hach Test Kit NICKEL

LCK337 EACH
EUR 183.86

Hach Test Kit SULPHIDE

LCK653 EACH
EUR 163.43

Elga Chlorine Test Kit

WAT4098 EACH
EUR 385.32

?-HCG (Human chorionic gonadotropin) ELISA test

1 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ?-HCG (Human chorionic gonadotropin)

HGH (Human chorionic gonadotropin) ELISA test

22 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HGH (Human chorionic gonadotropin)

NOVATest IgG/IgM Antibody Rapid Test Kit (NOVA Test)

UNCOV-40 40 Tests
EUR 115

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

Test product

testtt 0 Ask for price

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Human toluidine Red Unheated Serum Test (TRUST) ELISA Kit

abx257506-96tests 96 tests
EUR 764.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

NOVATest Antigen Rapid Test Kit (For Single Use) (NOVA Test)

nCov-500-01 1T
EUR 162

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

FSH (Human Follicle-stimulating hormone) ELISA test

3 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)

AFP (Alpha fetoprotein) ELISA test

6 96T/Box Ask for price
Description: ELISA based test for quantitative detection of AFP (Alpha fetoprotein)

CA199 (Cancer antigen) ELISA test

13 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA199 (Cancer antigen)

LH (Luteinizing Hormone) ELISA test

2 96T/Box Ask for price
Description: ELISA based test for quantitative detection of LH (Luteinizing Hormone)

CA153 (Cancer antigen) ELISA test

12 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA153 (Cancer antigen)

CA125 (Cancer antigen) ELISA test

11 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA125 (Cancer antigen)

CA50 (Cancer antigen) ELISA test

10 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA50 (Cancer antigen)

FT4 (Free thyroxine) ELISA test

18 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FT4 (Free thyroxine)

FT3 (Free triiodothyronine) ELISA test

17 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FT3 (Free triiodothyronine)

HEV antibody IgM ELISA test

92 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HEV antibody IgM

CEA (Cancer antigen) ELISA test

9 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CEA (Cancer antigen)

HAV antibody IgM ELISA test

75 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HAV antibody IgM

Human Echovirus (ECHO) IgM Rapid Test Kit

abx092093-20tests 20 tests
EUR 326.4

Human Toxoplasma (TOX) IgG Rapid Test Kit

abx092098-20tests 20 tests
EUR 260.4

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

Melamine (MEL) Rapid Test Kit

abx092057-50tests 50 tests
EUR 444

Clenbuterol (CLE) Rapid Test Kit

abx092058-50tests 50 tests
EUR 292.8

Ractopamine (RAC) Rapid Test Kit

abx092059-50tests 50 tests
EUR 292.8

Salbutamol (SAL) Rapid Test Kit

abx092060-50tests 50 tests
EUR 292.8

Tetracycline (TCs) Rapid Test Kit

abx092063-50tests 50 tests
EUR 477.6

Sulfonamides (Sas) Rapid Test Kit

abx092064-40tests 40 tests
EUR 477.6

Quinolones (QNs) Rapid Test Kit

abx092065-40tests 40 tests
EUR 477.6

Ciprofloxacin (CPFX) Rapid Test Kit

abx092066-50tests 50 tests
EUR 477.6

Quinolones (QNs) Rapid Test Kit

abx092067-40tests 40 tests
EUR 477.6

Brucella Antibody Rapid Test Kit

abx092069-40tests 40 tests
EUR 427.2

Antibody ISOTYPING TEST KIT (OKSA11303)

OKSA11303 10 Tests
EUR 936
Description: Description of target: MOUSE MONOCLONAL ANTIBODY ISOTYPING TEST KIT;Species reactivity: Mouse;Application: IS;Assay info: ;Sensitivity:

PCRAgH5 AIV Detection Test Kit

PD55-02 1 kit
EUR 1082.96
Description: Please check the datasheet of PCRAgH5 AIV Detection Test Kit before using the test.

Rapid Leishmania Ab Test Kit

RB2104 1 box
EUR 152.4
Description: Please check the datasheet of Rapid Leishmania Ab Test Kit before using the test.

Rapid PED Ag Test Kit

RG14-01 1 box
EUR 191.88
Description: Please check the datasheet of Rapid PED Ag Test Kit before using the test.

Thermal Test Film Starter Kit

PCR0892 EACH
EUR 275.88

Thermal Test Film Roll Kit

PCR0894 EACH
EUR 234.84

Hanna Test Kit Chlorine Free

WAT1572 EACH
EUR 30.78

Penicillin Drug Stability Test Kit

PST100 100 assays
EUR 231.22
Description: This product includes 0.5 ml of the 10 x PST dye for 100 assays using 96-well plates or micro-cuvettes.

Liposome Plasma Stability Test Kit

SPS20 20 columns
EUR 234.99
Description: This product includes 20 spin-columns for liposome stability studies in plasma

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 5929.2

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 5929.2

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

GEN-402-25tests 25 tests
EUR 292.8
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2558.4

DNASE TEST AGAR

D04-101-10kg 10 kg Ask for price

DNASE TEST AGAR

D04-101-2Kg 2 Kg Ask for price

DNASE TEST AGAR

D04-101-500g 500 g Ask for price

DERMATOPHYTE TEST AGAR

D04-107-10kg 10 kg
EUR 1018.8

DERMATOPHYTE TEST AGAR

D04-107-2Kg 2 Kg
EUR 268.8

DERMATOPHYTE TEST AGAR

D04-107-500g 500 g
EUR 116.4

MOTILITY TEST MEDIUM

M13-121-10kg 10 kg
EUR 1833.6

MOTILITY TEST MEDIUM

M13-121-2Kg 2 Kg
EUR 445.2

MOTILITY TEST MEDIUM

M13-121-500g 500 g
EUR 164.4

SNYDER TEST AGAR

S19-119-10kg 10 kg
EUR 1258.8

SNYDER TEST AGAR

S19-119-2Kg 2 Kg
EUR 320.4

SNYDER TEST AGAR

S19-119-500g 500 g
EUR 130.8

SAM Test Strip

TS00201s-30 30 tests
EUR 499.2
Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood

SAH Test Strip

TS00301s-30 30 tests
EUR 604.8
Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood

HCY Test Strip

TS00401s-30 30 tests
EUR 663.6
Description: Serum homocysteine quantitative test strip

Test Tube 16x100mm

3840/16X100 PK100
EUR 57.11

Dryslide Oxidase Test

231746 PK75
EUR 199.5

aa test product

123 1MG
EUR 279.6

Phenols Cuvette Test

LCK345 EACH
EUR 141.72

Dermatophyte Test Medium

MED1484 EACH
EUR 87.78

Test for Enteropathogenic.C

MIC6774 PK50
EUR 215.46

Sulphite Test Strips

MN91306 PK100
EUR 51.53

Thermal Test Film

PCR0890 PK25
EUR 383.04

5mL Test Tubes

TUB0228 PK1400
EUR 159.6

Spectroquant COD test

WAT1080 EACH
EUR 96.9

Phosphate Total Test

WAT3655 EACH
EUR 203.01

HPV (Human Papilloma Virus Antibody IgG) ELISA test

49 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HPV (Human Papilloma Virus Antibody IgG)

Human Hepatitis A Virus IgG Rapid Test Kit

abx092012-25tests 25 tests
EUR 510

Human Chlamydia Pneumoniae (CP) IgG Rapid Test Kit

abx092079-20tests 20 tests
EUR 309.6

Human Chlamydia Pneumoniae (CP) IgM Rapid Test Kit

abx092080-20tests 20 tests
EUR 309.6

Human Mycoplasma Pneumoniae (MP) IgG Rapid Test Kit

abx092081-20tests 20 tests
EUR 309.6

Human Mycoplasma Pneumoniae (MP) IgM Rapid Test Kit

abx092082-20tests 20 tests
EUR 309.6

Human Mycobacterium Tuberculosis (MT) IgG Rapid Test Kit

abx092084-20tests 20 tests
EUR 276

Human Helicobacter Pylori (HP) IgG Rapid Test Kit

abx092090-20tests 20 tests
EUR 276

Human Enterovirus 71 (EV71) IgM Rapid Test Kit

abx092091-20tests 20 tests
EUR 410.4

Human Hantavirus (HV) IgM / IgG Rapid Test Kit

abx092094-20tests 20 tests
EUR 326.4

Human Streptococcus Pneumoniae (SP) Antigen Rapid Test Kit

abx092096-20tests 20 tests
EUR 477.6

Human Rubella Virus (RV) IgG Rapid Test Kit

abx092097-20tests 20 tests
EUR 260.4

Human Rabies Virus (HRV) IgG Rapid Test Kit

abx092100-20tests 20 tests
EUR 410.4

Human Treponema Pallidum (TP) Antibody Rapid Test Kit

abx092101-20tests 20 tests
EUR 309.6

ACA (Anticardiolipin Antibody IgA) ELISA test

61 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgA)

Adv (Adenovirus Antibody IgM) ELISA test

44 96T/Box Ask for price
Description: ELISA based test for quantitative detection of Adv (Adenovirus Antibody IgM)

ANA (Antinuclear Antibody IgG) ELISA test

52 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ANA (Antinuclear Antibody IgG)

ACA (Anticardiolipin Antibody IgM) ELISA test

60 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgM)

COX (Coxsackievirus Antibody IgG) ELISA test

140 96T/Box Ask for price
Description: ELISA based test for quantitative detection of COX (Coxsackievirus Antibody IgG)

COX (Coxsackievirus Antibody IgM) ELISA test

141 96T/Box Ask for price
Description: ELISA based test for quantitative detection of COX (Coxsackievirus Antibody IgM)

TOX (Toxoplasma Antibody IgG) ELISA test

32 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgG)

ACA Anticardiolipin Ab IgA ELISA test

122 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ACA Anticardiolipin Ab IgA

IgE (Immune globulin E) ELISA test

21 96T/Box Ask for price
Description: ELISA based test for quantitative detection of IgE (Immune globulin)

TOX (Toxoplasma Antibody IgM) ELISA test

33 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgM)

RV (Rubella Antibody IgM) ELISA test

31 96T/Box Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgM)

CYFRA211 (Keratin 21-1) ELISA test

23 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CYFRA211 (Keratin 21-1)

IAA (Anti-insulin antibody) ELISA test

25 96T/Box Ask for price
Description: ELISA based test for quantitative detection of IAA (Anti-insulin antibody)

RV (Rubella Antibody IgG) ELISA test

30 96T/Box Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgG)

CMV (Cytomegalovirus Antibody IgM) ELISA test

29 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgM)

CMV (Cytomegalovirus Antibody IgG) ELISA test

28 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgG)

PSA (Prostate-specific antigen) ELISA test

8 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PSA (Prostate-specific antigen)

Syphilis anti-TP syphilis ELISA test

99 96T/Box Ask for price
Description: ELISA based test for quantitative detection of Syphilis anti-TP syphilis

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 513.6

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 472.8

Clenbuterol Rapid Test Kit (Colloidal gold)

abx092040-50tests 50 tests
EUR 326.4

Moleculaire cytogenetische analyse en het opzetten van een celkweek bij de vissoort Hollandichthys multifasciatus (Eigenmann & amp; Norris, 1900) (Characiformes, Characidae)

Hollandichthys is a fish genus of the family Characidae that was until recently considered to be monotypic, with cytogenetic, morphological, and molecular data being restricted to a few local populations. In the present study, the karyotype of a population of Hollandichthys multifasciatus was analyzed using classical and molecular cytogenetic approaches for the investigation of potential markers that could provide new perspectives on the cytotaxonomy. H. multifasciatus presented a diploid number of 2n=50 chromosomes and a karyotype formula of 8m+10sm+32st. A single pair of chromosomes presented Ag-NORs signals, which coincided with the 18S rDNA sites visualized by FISH, whilst the 5S rDNA sequences were mapped in two chromosome pairs. The distribution of the U snRNA genes was mapped on the Hollandichthys chromosomes for the first time, with the probes revealing the presence of the U1 snDNA on the chromosomes of pair 20, U2 on pairs 6 and 19, U4 on pair 16, and U6 on the chromosomes of pair 11. The results of the present study indicated karyotypic differences in comparison with the other populations of H. multifasciatus studied previously, reinforcing the need for further research to identify isolated populations or the potential existence of cryptic Hollandichthys species.
• The effects of salt stress and adaptation on salicylic acid (SA) content and on antioxidant and lipoxygenase (LOX) enzyme activities were studied in tomato (Lycopersicon esculentum cv. Pera) cells. • NaCl-adapted cells were obtained from calli adapted to 100 mm NaCl by successive subcultures in medium supplemented with 100 mm NaCl. Salt stress treatments consisted of the addition of 100 mm NaCl to cells. • Adapted cells contained a lower concentration of SA than unadapted cells. The lower manganese-containing superoxide dismutase (Mn-SOD) and LOX activities as well as the higher glutathione reductase (GR) and ascorbate peroxidase (APX) activities in adapted cells than in unadapted cells could be correlated with the development of salt adaptation. Salt stress increased APX and LOX activities as well as lipid peroxidation in unadapted cells and increased Mn-SOD activity in both types of cells. Application of 200 µm SA + 100 mm NaCl inhibited APX activity in both unadapted and adapted cells, induced the Mn-SOD in adapted cells and increased lipid peroxidation in unadapted cells. • Our data indicate that adaptation of tomato cells to NaCl results in a higher tolerance to NaCl-induced oxidative stress and suggest a role for SA in this response.

Een reactie achterlaten

Het e-mailadres wordt niet gepubliceerd. Vereiste velden zijn gemarkeerd met *