Toxiciteit van combinaties van remmers van de kinaseroute voor normale menselijke cellen in een driedimensionale cultuur
Resistance to single-agent chemotherapy and molecularly targeted drugs prevents sustained efficacy of treatments. To address this challenge, combination drug treatments have been used to improve outcomes for patients. Potential toxicity of combination treatments is a major concern, however, and has led to the failure of several clinical trials in different cancers. The use of cell-based models of normal tissues in preclinical studies enables testing and identifying toxic effects of drug combinations and facilitates an informed decision-making process for advancing the treatments to animal models and clinical trials. Recently, we established that combinations of molecular inhibitors of mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase-protein kinase B (PI3K/Akt) pathways effectively and synergistically inhibit growth of BRAFmut and KRASmut colorectal tumor spheroids by blocking feedback signaling of downstream kinase pathways. These pathways are important for cell proliferation, however, and their simultaneous inhibition may cause toxicity to normal cells. We used a cellular spheroid model to study toxicities of drug combinations to human bone marrow and colon. Our results indicated that MAPK and PI3K/Akt inhibitors used simultaneously were only moderately toxic to bone marrow cells but significantly more toxic to colon cells. Our molecular analysis of proliferative cell activities and housekeeping proteins further corroborated these results. Overall, our approach to identify toxic effects of combinations of cancer drugs to normal cells in three-dimensional cultures will facilitate more informed treatment selections for subsequent animal studies.
Gehumaniseerd kweekmedium voor het genereren van klinische kwaliteit van erytroïde cellen uit navelstrengbloed CD34 + cellen
Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost.
Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated.
Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99±17×103-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium.
Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation.
 ELISA Test Kit) Tetrodotoxin (TTX) ELISA Test Kit |
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| DEIANJ48 | Creative Diagnostics | 96T | EUR 1625 |
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Description: This test kit is for the quantitative detection of Tetrodotoxin in the tissue, liver, fish. |
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 CA72-4 ELISA test |
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| 24 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CA72-4 |
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 ELISA test) FERR ( Ferritin) ELISA test |
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| 7 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of FERR ( Ferritin) |
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 ELISA test) TSH (Thyrotrophin) ELISA test |
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| 14 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of TSH (Thyrotrophin) |
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 ELISA test) T3 (Triiodothyronine) ELISA test |
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| 15 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of T3 (Triiodothyronine) |
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 ELISA test) T4 (Thyroxine) ELISA test |
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| 16 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of T4 (Thyroxine) |
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 ELISA test) TESTO (Testosterone) ELISA test |
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| 19 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of O (osterone) |
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 ELISA test) PROG (Progesterone) ELISA test |
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| 20 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of PROG (Progesterone) |
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 ELISA test) E2 (Estradiol) ELISA test |
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| 5 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of E2 (Estradiol) |
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 ELISA test) PRL (Prolactin) ELISA test |
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| 4 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of PRL (Prolactin) |
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 PreS2-Ag ELISA test |
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| 96 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of PreS2-Ag |
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 HBcAb Sandwich ELISA test |
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| 95 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of HBcAb Sandwich |
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 Melamine Rapid Test Kit |
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| abx092011-50tests | Abbexa | 50 tests | EUR 370 |
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 NDV rapid test kit |
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| RG15-03 | Bionote | 1 box | EUR 139.05 |
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Description: Please check the datasheet of NDV rapid test kit before using the test. |
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 ELISA test) HGH (Human chorionic gonadotropin) ELISA test |
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| 22 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of HGH (Human chorionic gonadotropin) |
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 Accu-Tell COVID-19 IgG/IgM Rapid Test |
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| GEN-B352-20tests | Accu test | 20 tests | EUR 236 |
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Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
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Accu-Tell COVID-19 IgG/IgM Rapid Test |
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| GEN-B352-40tests | Accu test | 40 tests | EUR 321 |
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Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
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 Frit Kit |
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| FRIT-KIT | Next Advance | 1each | EUR 124 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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 Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | EUR 1035 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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 PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | EUR 266 |
 ELISA Kit) Human toluidine Red Unheated Serum Test (TRUST) ELISA Kit |
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| abx257506-96tests | Abbexa | 96 tests | EUR 637 |
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 ELISA test) CA125 (Cancer antigen) ELISA test |
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| 11 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CA125 (Cancer antigen) |
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 ELISA test) CA199 (Cancer antigen) ELISA test |
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| 13 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CA199 (Cancer antigen) |
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 ELISA test) FT3 (Free triiodothyronine) ELISA test |
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| 17 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of FT3 (Free triiodothyronine) |
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 ELISA test) FT4 (Free thyroxine) ELISA test |
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| 18 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of FT4 (Free thyroxine) |
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 ELISA test) LH (Luteinizing Hormone) ELISA test |
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| 2 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of LH (Luteinizing Hormone) |
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 ELISA test) CA153 (Cancer antigen) ELISA test |
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| 12 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CA153 (Cancer antigen) |
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 ELISA test) CA50 (Cancer antigen) ELISA test |
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| 10 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CA50 (Cancer antigen) |
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 ELISA test) AFP (Alpha fetoprotein) ELISA test |
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| 6 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of AFP (Alpha fetoprotein) |
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 ELISA test) CEA (Cancer antigen) ELISA test |
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| 9 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CEA (Cancer antigen) |
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 HAV antibody IgM ELISA test |
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| 75 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of HAV antibody IgM |
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 HEV antibody IgM ELISA test |
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| 92 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of HEV antibody IgM |
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 Rapid Test Kit) Melamine (MEL) Rapid Test Kit |
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| abx092057-50tests | Abbexa | 50 tests | EUR 370 |
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 Rapid Test Kit) Clenbuterol (CLE) Rapid Test Kit |
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| abx092058-50tests | Abbexa | 50 tests | EUR 244 |
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 Rapid Test Kit) Ractopamine (RAC) Rapid Test Kit |
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| abx092059-50tests | Abbexa | 50 tests | EUR 244 |
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 Rapid Test Kit) Salbutamol (SAL) Rapid Test Kit |
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| abx092060-50tests | Abbexa | 50 tests | EUR 244 |
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 Rapid Test Kit) Tetracycline (TCs) Rapid Test Kit |
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| abx092063-50tests | Abbexa | 50 tests | EUR 398 |
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 Rapid Test Kit) Sulfonamides (Sas) Rapid Test Kit |
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| abx092064-40tests | Abbexa | 40 tests | EUR 398 |
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 Rapid Test Kit) Quinolones (QNs) Rapid Test Kit |
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| abx092065-40tests | Abbexa | 40 tests | EUR 398 |
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 Rapid Test Kit) Ciprofloxacin (CPFX) Rapid Test Kit |
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| abx092066-50tests | Abbexa | 50 tests | EUR 398 |
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Quinolones (QNs) Rapid Test Kit |
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| abx092067-40tests | Abbexa | 40 tests | EUR 398 |
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 Brucella Antibody Rapid Test Kit |
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| abx092069-40tests | Abbexa | 40 tests | EUR 356 |
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 PCRAgH5 AIV Detection Test Kit |
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| PD55-02 | Bionote | 1 kit | EUR 902.47 |
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Description: Please check the datasheet of PCRAgH5 AIV Detection Test Kit before using the test. |
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 Rapid Leishmania Ab Test Kit |
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| RB2104 | Bionote | 1 box | EUR 127 |
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Description: Please check the datasheet of Rapid Leishmania Ab Test Kit before using the test. |
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 Rapid PED Ag Test Kit |
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| RG14-01 | Bionote | 1 box | EUR 159.9 |
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Description: Please check the datasheet of Rapid PED Ag Test Kit before using the test. |
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) Antibody ISOTYPING TEST KIT (OKSA11303) |
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| OKSA11303 | Aviva Systems Biology | 10 Tests | EUR 780 |
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Description: Description of target: MOUSE MONOCLONAL ANTIBODY ISOTYPING TEST KIT;Species reactivity: Mouse;Application: IS;Assay info: ;Sensitivity: |
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 ELISA test) FSH (Human Follicle-stimulating hormone) ELISA test |
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| 3 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone) |
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 IgM Rapid Test Kit) Human Echovirus (ECHO) IgM Rapid Test Kit |
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| abx092093-20tests | Abbexa | 20 tests | EUR 272 |
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 IgG Rapid Test Kit) Human Toxoplasma (TOX) IgG Rapid Test Kit |
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| abx092098-20tests | Abbexa | 20 tests | EUR 217 |
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 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110 |
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 DNASE TEST AGAR |
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| D04-101-10kg | Alphabiosciences | 10 kg | Ask for price |
DNASE TEST AGAR |
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| D04-101-2Kg | Alphabiosciences | 2 Kg | Ask for price |
DNASE TEST AGAR |
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| D04-101-500g | Alphabiosciences | 500 g | Ask for price |
 DERMATOPHYTE TEST AGAR |
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| D04-107-10kg | Alphabiosciences | 10 kg | EUR 849 |
DERMATOPHYTE TEST AGAR |
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| D04-107-2Kg | Alphabiosciences | 2 Kg | EUR 224 |
DERMATOPHYTE TEST AGAR |
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| D04-107-500g | Alphabiosciences | 500 g | EUR 97 |
 MOTILITY TEST MEDIUM |
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| M13-121-10kg | Alphabiosciences | 10 kg | EUR 1528 |
MOTILITY TEST MEDIUM |
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| M13-121-2Kg | Alphabiosciences | 2 Kg | EUR 371 |
MOTILITY TEST MEDIUM |
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| M13-121-500g | Alphabiosciences | 500 g | EUR 137 |
 SNYDER TEST AGAR |
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| S19-119-10kg | Alphabiosciences | 10 kg | EUR 1049 |
SNYDER TEST AGAR |
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| S19-119-2Kg | Alphabiosciences | 2 Kg | EUR 267 |
SNYDER TEST AGAR |
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| S19-119-500g | Alphabiosciences | 500 g | EUR 109 |
 SAM Test Strip |
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| TS00201s-30 | Arthus | 30 tests | EUR 416 |
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Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood |
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 SAH Test Strip |
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| TS00301s-30 | Arthus | 30 tests | EUR 504 |
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Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood |
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 HCY Test Strip |
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| TS00401s-30 | Arthus | 30 tests | EUR 553 |
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Description: Serum homocysteine quantitative test strip |
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 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV100PA-KIT | SBI | 1 Kit | EUR 1132 |
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 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV105PA-KIT | SBI | 1 Kit | EUR 1132 |
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 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
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 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV125PA-KIT | SBI | 1 Kit | EUR 1132 |
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 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS700A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS720A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS740A-KIT | SBI | 10 rxn | EUR 1132 |
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) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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| CAS510A-KIT | SBI | 1 Kit | EUR 805 |
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV200PA-KIT | SBI | 1 Kit | EUR 1132 |
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-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV205PA-KIT | SBI | 1 Kit | EUR 1132 |
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
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-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV225PA-KIT | SBI | 1 Kit | EUR 1132 |
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) 2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) |
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| GEN-402-25tests | All test | 25 tests | EUR 244 |
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Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
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 Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS750A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS770A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS790A-KIT | SBI | 10 rxn | EUR 1132 |
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 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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| CAS9LIG-KIT | SBI | 1 Kit | EUR 153 |
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 ELISA test) CMV (Cytomegalovirus Antibody IgM) ELISA test |
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| 29 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgM) |
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 ELISA test) RV (Rubella Antibody IgG) ELISA test |
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| 30 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgG) |
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 ELISA test) CYFRA211 (Keratin 21-1) ELISA test |
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| 23 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CYFRA211 (Keratin 21-1) |
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 ELISA test) IAA (Anti-insulin antibody) ELISA test |
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| 25 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of IAA (Anti-insulin antibody) |
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 ELISA test) IgE (Immune globulin E) ELISA test |
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| 21 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of IgE (Immune globulin) |
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 ELISA test) RV (Rubella Antibody IgM) ELISA test |
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| 31 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgM) |
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 ELISA test) CMV (Cytomegalovirus Antibody IgG) ELISA test |
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| 28 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgG) |
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 ELISA test) TOX (Toxoplasma Antibody IgM) ELISA test |
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| 33 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgM) |
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 ELISA test) TOX (Toxoplasma Antibody IgG) ELISA test |
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| 32 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgG) |
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 ELISA test) ACA (Anticardiolipin Antibody IgA) ELISA test |
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| 61 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgA) |
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 ELISA test) ANA (Antinuclear Antibody IgG) ELISA test |
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| 52 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of ANA (Antinuclear Antibody IgG) |
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 ELISA test) ACA (Anticardiolipin Antibody IgG) ELISA test |
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| 59 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgG) |
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 ELISA test) ACA (Anticardiolipin Antibody IgM) ELISA test |
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| 60 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgM) |
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 ELISA test) Adv (Adenovirus Antibody IgM) ELISA test |
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| 44 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of Adv (Adenovirus Antibody IgM) |
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 ELISA test) PSA (Prostate-specific antigen) ELISA test |
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| 8 | Biobase | 96T/Box | Ask for price |
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Description: ELISA based test for quantitative detection of PSA (Prostate-specific antigen) |
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Moleculaire cytogenetische analyse en het opzetten van een celkweek bij de vissoort Hollandichthys multifasciatus (Eigenmann & amp; Norris, 1900) (Characiformes, Characidae)
Hollandichthys is a fish genus of the family Characidae that was until recently considered to be monotypic, with cytogenetic, morphological, and molecular data being restricted to a few local populations. In the present study, the karyotype of a population of Hollandichthys multifasciatus was analyzed using classical and molecular cytogenetic approaches for the investigation of potential markers that could provide new perspectives on the cytotaxonomy. H. multifasciatus presented a diploid number of 2n=50 chromosomes and a karyotype formula of 8m+10sm+32st. A single pair of chromosomes presented Ag-NORs signals, which coincided with the 18S rDNA sites visualized by FISH, whilst the 5S rDNA sequences were mapped in two chromosome pairs. The distribution of the U snRNA genes was mapped on the Hollandichthys chromosomes for the first time, with the probes revealing the presence of the U1 snDNA on the chromosomes of pair 20, U2 on pairs 6 and 19, U4 on pair 16, and U6 on the chromosomes of pair 11. The results of the present study indicated karyotypic differences in comparison with the other populations of H. multifasciatus studied previously, reinforcing the need for further research to identify isolated populations or the potential existence of cryptic Hollandichthys species.
• The effects of salt stress and adaptation on salicylic acid (SA) content and on antioxidant and lipoxygenase (LOX) enzyme activities were studied in tomato (Lycopersicon esculentum cv. Pera) cells. • NaCl-adapted cells were obtained from calli adapted to 100 mm NaCl by successive subcultures in medium supplemented with 100 mm NaCl. Salt stress treatments consisted of the addition of 100 mm NaCl to cells. • Adapted cells contained a lower concentration of SA than unadapted cells. The lower manganese-containing superoxide dismutase (Mn-SOD) and LOX activities as well as the higher glutathione reductase (GR) and ascorbate peroxidase (APX) activities in adapted cells than in unadapted cells could be correlated with the development of salt adaptation. Salt stress increased APX and LOX activities as well as lipid peroxidation in unadapted cells and increased Mn-SOD activity in both types of cells. Application of 200 µm SA + 100 mm NaCl inhibited APX activity in both unadapted and adapted cells, induced the Mn-SOD in adapted cells and increased lipid peroxidation in unadapted cells. • Our data indicate that adaptation of tomato cells to NaCl results in a higher tolerance to NaCl-induced oxidative stress and suggest a role for SA in this response.