fondationvocation

van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel

Kwantitatieve bepaling van sporenmetaal in celkweekmedia met behulp van LS-APGD-MS en ICP-OES met differentiatie van vrije / gebonden soorten na scheidingen van polymeervezels

Liquid sampling-atmospheric pressure glow discharge-mass spectrometry (LS-APGD-MS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES) were employed for the quantification of trace metals in cell culture media and their capabilities compared. The LS-APGD is interfaced here to a compact mass spectrometer (Advion CMS) towards the development of an at-bioreactor process monitoring strategy. Both techniques have been previously employed for the quantification of trace metals in samples of various complexities, making them a natural choice for this application.

They have also demonstrated comparable analytical figures of merit including limits of detection (LOD), matrix tolerance, etc. While cell culture media is a complex sample, the ICP-OES technique was unaffected by the matrix. However, the LS-APGD-MS suffered from increases in spectral background. Despite this, both techniques achieved appropriate LODs for all metals analyzed in this work (Cu, Fe, Zn, Co, Mn, Ni; LOD < 100 ng mL-1), except for Mn and Ni via LS-APGD-MS. To overcome the increased background seen on the LS-APGD-MS, a capillary channeled polymer (C-CP) polypropylene (PPY) fiber stationary phase was employed as an on-line separation for the removal of organic components prior to sample introduction into the plasma.
It was further determined that Ni was retained on the column, preventing the detection of this element via LS-APGD-MS, and insights into metal speciation were discussed. Following implementation of this on-line separation strategy, the agreement between the techniques was acceptable for all analytes, and was excellent for Cu, Fe, and Zn.

Co-gereguleerde pilus van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel van Caco-2 en perifere bloedmononucleaire cellen (PBMC), wat leidt tot verhoogde kolonisatie

The aim of this study was to assess the modulatory effect of TcpA in the expression of CEACAM1 adhesin molecule and IL-1, IL-8 and TNFα pro-inflammatiory cytokines in the co-culture model of Caco-2/PBMC that can mimic the intestinal milieu. The tcpA gene from V.cholerae ATCC14035 was cloned in pET-28a and transformed into E.coli Bl-21. The recombinant TcpA-His6 protein was expressed and purified using the Ni-column chromatography.
The sequencing of transformed plasmid and Western blotting of purified protein confirmed the identity of rTcp. Cytotoxicity of different concentrations of recombinant protein for human colon carcinoma cell line (Caco-2 cell) was assessed by MTT assay and showed viability of 92%, 82% and 70 %, for10 µg/mL of TcpA after 24, 48 and 72 h, respectively. Co-cultures of Caco-2 and peripheral blood mononuclear cells (PBMCs) were used to mimic the intestinal milieu and treated with different concentrations of rTcpA (1, 5, 10 and 50 µg/mL).
Our data showed about 2.04, 3.37, 3.68 and 42.7-folds increase in CEACAM1 gene expression, respectively, compared with non-treated Caco-2/PBMC co-culture. Moreover, the expression of IL-1, IL-8 and TNFα genes was significantly increased up to 15.75, 7.04 and 80.95 folds, respectively. In conclusion, V.cholerae TcpA induces statistically significant dose-dependent stimulatory effect on TNFα, IL-1 and IL-8 pro-inflammatory cytokines expression. Among all, TNFα was much more affected which consequently, elevated CEACAM1 expression level in IECs. This suggests TcpA protein as critical effector as an inducer of increased adhesion potential of V. cholera as well as inflammatory responses of host intestinal tissue. This article is protected by copyright. All rights reserved.
fondationvocation
fondationvocation

pAAV-DJ vector

PVT12151 2 ug
EUR 438

pAAV-DJ Vector

VPK-420-DJ 10 µg
EUR 647
Description: The pAAV-DJ vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ packaging.

pAAV-RC1 Vector

VPK-421 10 µg
EUR 647
Description: The pAAV-RC1 vector contains the rep and cap genes required to generated recombinant AAV of serotype 1. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-1 packaging.

pAAV-RC3 Vector

VPK-423 10 µg
EUR 647
Description: The pAAV-RC3 vector contains the rep and cap genes required to generated recombinant AAV of serotype 3. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-3 packaging.

pAAV-RC4 Vector

VPK-424 10 µg
EUR 647
Description: The pAAV-RC4 vector contains the rep and cap genes required to generated recombinant AAV of serotype 4. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-4 packaging.

pAAV-RC5 Vector

VPK-425 10 µg
EUR 647
Description: The pAAV-RC5 vector contains the rep and cap genes required to generated recombinant AAV of serotype 5. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-5 packaging.

pAAV-RC6 Vector

VPK-426 10 µg
EUR 647
Description: The pAAV-RC6 vector contains the rep and cap genes required to generated recombinant AAV of serotype 6. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-6 packaging.

pAAV-RC6

PVT14647 2 ug
EUR 703

pAAV- RC

PVT2103 2 ug
EUR 241

pAAV-GFP Control Vector

AAV-400 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-Cre Control Vector

AAV-401 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-LacZ Control Vector

AAV-402 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-DJ/8 Vector

VPK-420-DJ-8 10 µg
EUR 647
Description: The pAAV-DJ/8 vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ/8. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ/8 packaging.

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV- IRES- ZsGreen1

PVT11044 2 ug
EUR 301

pAAV- ZsGreen1- shRNA

PVT11045 2 ug
EUR 370

pAAV-fNPY-GFP

PVT14636 2 ug
EUR 599

pAAV-RSV-SpCas9

PVT17629 2 ug
EUR 300

pAAV-CAG-GFP

PVT17666 2 ug
EUR 341

pAAV- MCS Plasmid

PVT2102 2 ug
EUR 241

pAAV-EF1a-DIO-mCherry

PVT17841 2 ug
EUR 300

pAAV- IRES- hrGFP Plasmid

PVT2104 2 ug
EUR 266

Human Topoisomerase I

TG2005H-RC2 500 units
EUR 448

pAAV- CMV- mCherry- U6- sgRNA

PVT11046 2 ug
EUR 301

pAAV-hSyn-hChR2(H134R)-mCherry

PVT19026 2 ug
EUR 258

pAAV-hSyn-eNpHR 3.0-EYFP

PVT19063 2 ug
EUR 258

pAAV-EF1a-DIO-hM3D(Gq)-mCherry

PVT17847 2 ug
EUR 300

pAAV-MCS-Ppargc1a-m-FLAG-HA

PVT18341 2 ug
EUR 300

pEE14.4 vector

PVT11901 2 ug
EUR 1036

pENTR223.1 vector

PVT11990 2 ug
EUR 352

pUB_smFLAG_KDM5B_MS2 vector

PVT11991 2 ug
EUR 352

ER2738 vector

PVT11993 2 ug
EUR 352

pFLPo vector

PVT12063 2 ug
EUR 352

pREDKI vector

PVT12064 2 ug
EUR 352

pREDCas9 vector

PVT12065 2 ug
EUR 352

PWUR790 vector

PVT12066 2 ug
EUR 352

xCas9 vector

PVT12068 2 ug
EUR 352

sgRNA vector

PVT12071 2 ug
EUR 352

PY094 vector

PVT12150 2 ug
EUR 352

PY094 vector

PVT12150-1 2 ug
EUR 352

pRGEB31 vector

PVT12152 2 ug
EUR 352

pSET152 vector

PVT3395 2 ug
EUR 376

pVL1392 Vector

ABP-BVP-10001 5 ug Ask for price

pVL1393 Vector

ABP-BVP-10002 5 ug Ask for price

pORB Vector

ABP-BVP-10003 5 ug Ask for price

pAcSec1 Vector

ABP-BVP-10004 5 ug Ask for price

pAcIRES Vector

ABP-BVP-10005 5 ug Ask for price

pENTR223-ATP5PO vector

PVT11685 2 ug
EUR 304

pENTR223- LC25A10 vector

PVT11686 2 ug
EUR 304

pENTR223-RSU1 vector

PVT11687 2 ug
EUR 304

pENTR223-CCDC24 vector

PVT11688 2 ug
EUR 304

pENTR223-BAT2L vector

PVT11689 2 ug
EUR 304

pENTR223-RSPH14 vector

PVT11690 2 ug
EUR 304

pENTR223-ABHD5 vector

PVT11691 2 ug
EUR 304

pENTR223-NONO vector

PVT11692 2 ug
EUR 304

pENTR223-MYOZ1 vector

PVT11693 2 ug
EUR 304

pENTR223-STX5 vector

PVT11694 2 ug
EUR 304

pENTR223-GK5 vector

PVT11695 2 ug
EUR 304

pENTR223-PIGC vector

PVT11696 2 ug
EUR 304

pENTR223-FCGR3A vector

PVT11697 2 ug
EUR 304

pENTR223-ANXA8L1 vector

PVT11698 2 ug
EUR 304

pENTR223-CDK5 vector

PVT11699 2 ug
EUR 304

pENTR223-ERP27 vector

PVT11700 2 ug
EUR 304

pENTR223-ZDHHC16 vector

PVT11701 2 ug
EUR 304

pENTR223-DFNA5 vector

PVT11702 2 ug
EUR 304

pENTR223-MPPED2 vector

PVT11703 2 ug
EUR 304

pENTR223-NIFK vector

PVT11704 2 ug
EUR 304

pENTR223-KCTD17 vector

PVT11705 2 ug
EUR 304

pENTR223-CEP170P1 vector

PVT11706 2 ug
EUR 304

pENTR223-SCML4 vector

PVT11707 2 ug
EUR 304

pENTR223-CLEC18C vector

PVT11708 2 ug
EUR 304

pENTR223-HSD17B11 vector

PVT11709 2 ug
EUR 304

pENTR223-UBXN1 vector

PVT11710 2 ug
EUR 304

pENTR223-MRPL4 vector

PVT11714 2 ug
EUR 304

pENTR223-DECR2 vector

PVT11715 2 ug
EUR 304

pENTR223-GNB3 vector

PVT11716 2 ug
EUR 304

pENTR223-FAM118B vector

PVT11717 2 ug
EUR 304

pENTR223-EIF2S2 vector

PVT11718 2 ug
EUR 304

pENTR223-HDAC11 vector

PVT11720 2 ug
EUR 304

pENTR223-RDH11 vector

PVT11721 2 ug
EUR 304

pENTR223-GNB1L vector

PVT11722 2 ug
EUR 304

pENTR223-BCL2L14 vector

PVT11723 2 ug
EUR 304

pENTR223-RPLP0 vector

PVT11724 2 ug
EUR 304

pENTR223-RALYL vector

PVT11725 2 ug
EUR 304

pENTR223-GPM6A vector

PVT11726 2 ug
EUR 304

pENTR223-BLVRA vector

PVT11727 2 ug
EUR 304

pENTR223-CDCP1 vector

PVT11729 2 ug
EUR 304

pENTR223-BTN2A2 vector

PVT11730 2 ug
EUR 304

pENTR223-MAX vector

PVT11731 2 ug
EUR 304

pENTR223-CRAT vector

PVT11732 2 ug
EUR 304

pENTR223-FKBPL vector

PVT11733 2 ug
EUR 304

pENTR223-SIAT7E vector

PVT11736 2 ug
EUR 304

pENTR223-AATF vector

PVT11737 2 ug
EUR 304

pENTR223-PEF1 vector

PVT11742 2 ug
EUR 304

pENTR223-INKA2 vector

PVT11743 2 ug
EUR 304

pENTR223-C5AR1 vector

PVT11745 2 ug
EUR 304

pENTR223-UBE2V1 vector

PVT11752 2 ug
EUR 304

pENTR223-USP47 vector

PVT11753 2 ug
EUR 304

pENTR223-MCFD2 vector

PVT11754 2 ug
EUR 304

pENTR223-PFDN2 vector

PVT11755 2 ug
EUR 304

pENTR223-HSP90AB1 vector

PVT11756 2 ug
EUR 304

pENTR223-AFAP1 vector

PVT11757 2 ug
EUR 304

pENTR223-STAT5A vector

PVT11761 2 ug
EUR 304

pENTR223-PRDM4 vector

PVT11762 2 ug
EUR 304

pENTR223-NGEF vector

PVT11763 2 ug
EUR 304

pENTR223-MAP7 vector

PVT11764 2 ug
EUR 304

pENTR223-EXT1 vector

PVT11765 2 ug
EUR 304

pENTR223-CARS vector

PVT11766 2 ug
EUR 304

pENTR223-STAT1 vector

PVT11767 2 ug
EUR 304

pENTR223-TMEM108 vector

PVT11768 2 ug
EUR 304

pENTR223-WDR1 vector

PVT11769 2 ug
EUR 304

pENTR223-POMT2 vector

PVT11770 2 ug
EUR 304

pENTR223-CLPB vector

PVT11771 2 ug
EUR 304

pENTR223-KIF1B vector

PVT11772 2 ug
EUR 304

pENTR223-MSTO1 vector

PVT11773 2 ug
EUR 304

pENTR223-DVL3 vector

PVT11775 2 ug
EUR 304

pENTR223-IGHM vector

PVT11776 2 ug
EUR 304

pENTR223-CFB vector

PVT11779 2 ug
EUR 304

pENTR223-TTC14 vector

PVT11780 2 ug
EUR 304

pENTR223-USHBP1 vector

PVT11781 2 ug
EUR 304

pENTR223-ATG7 vector

PVT11783 2 ug
EUR 304

pENTR223-CPT1A vector

PVT11785 2 ug
EUR 304

pENTR223-YY1AP1 vector

PVT11786 2 ug
EUR 304

pENTR223-PFKM vector

PVT11787 2 ug
EUR 304

pENTR223-FES vector

PVT11789 2 ug
EUR 304

pENTR223-PIGQ vector

PVT11790 2 ug
EUR 304

pENTR223-RARS vector

PVT11791 2 ug
EUR 304

pENTR223-MAN1B1 vector

PVT11792 2 ug
EUR 304

pENTR223-HSPA2 vector

PVT11793 2 ug
EUR 304

pENTR223-ARAF vector

PVT11794 2 ug
EUR 304

pENTR223-PIAS3 vector

PVT11795 2 ug
EUR 304

pENTR223-WBP11 vector

PVT11797 2 ug
EUR 304

pENTR223-LGALS3BP vector

PVT11798 2 ug
EUR 304

pENTR223-GTF3C2 vector

PVT11799 2 ug
EUR 304

pENTR223-KLHL12 vector

PVT11800 2 ug
EUR 304

pENTR223-MEN1 vector

PVT11801 2 ug
EUR 304

pENTR223-PCM1 vector

PVT11802 2 ug
EUR 304

pENTR223-SLC43A1 vector

PVT11803 2 ug
EUR 304

pENTR223-NRBP1 vector

PVT11804 2 ug
EUR 304

pENTR223-NAE1 vector

PVT11805 2 ug
EUR 304

pENTR223-CCDC28A vector

PVT11806 2 ug
EUR 304

pENTR223-UBC vector

PVT11807 2 ug
EUR 304

pENTR223-PFN1 vector

PVT11808 2 ug
EUR 304

pENTR223-TMEM239 vector

PVT11809 2 ug
EUR 304

pENTR223-CDH16 vector

PVT11810 2 ug
EUR 304

pENTR223-PMCH vector

PVT11811 2 ug
EUR 304

pENTR223-WDR63 vector

PVT11812 2 ug
EUR 304

pENTR223-PLA2G16 vector

PVT11813 2 ug
EUR 304

pENTR223-CEP290 vector

PVT11814 2 ug
EUR 304

pENTR223-RARRES2 vector

PVT11815 2 ug
EUR 304

pENTR223-ATG12 vector

PVT11816 2 ug
EUR 304

pENTR223-ERG28 vector

PVT11817 2 ug
EUR 304

pENTR223-ARL2BP vector

PVT11819 2 ug
EUR 304

pENTR223-PITRM1 vector

PVT11821 2 ug
EUR 304

pENTR223-WRAP53 vector

PVT11822 2 ug
EUR 304

pENTR223-MRPL3 vector

PVT11824 2 ug
EUR 304

pENTR223-SMUG1 vector

PVT11825 2 ug
EUR 304

pENTR223-CCL13 vector

PVT11826 2 ug
EUR 304

pENTR223-NDUFA5 vector

PVT11829 2 ug
EUR 304

Een nieuwe isolatie- en kweekmethode voor granulosacellen

Granulosa cells are the cell population who have an increasing importance in the female genital system and reproduction. Thus, nowadays in vitro studies to address these cells are also gaining importance and attracts researcher’s attention. The aim of our study is to develop a more feasible, low-cost granulosa cell isolation and culture method compared to methods defined so far.
Granulosa cells were isolated from follicular fluids obtained from both healthy women donors (n = 19) and polycystic ovary syndrome (n = 15) applied to in vitro fertilization treatment process. Granulosa cells were isolated by using Lymphosep® separation fluid that was not used for this purpose before. The isolated cells were cultured in suitable culture dishes with a mixture of BIO-AMFTM-1 and DMEM/F12 in the first seeding and only complete DMEM/F12 in the following feeds.
  • The complete medium contains only 5% fetal calf serum, 4% L-glutamine and 1% penicillin-streptomycin-amphotericin. The new methods we have developed in granulosa cell isolation and in vitro culture have been successful.
  • Reduction in supplement types and amount; improved the proliferation rate of the granulosa cells in culture. Our new methods of isolation and cell culture for granulosa cells from healthy women, have been also successful in samples of polycystic ovarian patients.
  • With these developed methods, granulosa cells, which belong to humans and have an important role in the ovary, could be isolated and subsequently be maintained to reproduce (proliferate) more easily and cheaper.

Over de oorsprong van SARS-CoV-2: hebben celkweekexperimenten geleid tot verhoogde virulentie van het voorlopervirus voor mensen?

We are currently in a rapidly expanding pandemic of the SARS-CoV-2 virus, which originated in the city of Wuhan in central China. The disease COVID-19 is now spread worldwide and has tremendous socio-economic consequences. The origin of the virus can be reconstructed through epidemiological studies and, even more so, from genome comparisons. How the evolution of the virus and the transition to humans might have happened is the subject of much speculation.
It is considered certain that the virus is of animal origin and very likely passed from bats to humans in a zoonotic event. An intermediate host was postulated, but many SARS-like bat viruses have the ability to infect human cells directly, which has been shown experimentally by scientists in the Wuhan Institute of Virology using collected specimens containing virus material from horseshoe bats.
The propagation of SARS-like bat viruses in cell culture allowed experiments aimed at increasing the infectivity of the virus and adaptation to human cells. This article summarizes the unique properties of SARS-CoV-2 and focusses on a specific sequence encoding the spike protein.
Possible scenarios of virus evolution are discussed, with particular emphasis on the hypothesis that the virus could have emerged unintentionally through routine culture or gain-of-function experiments in a laboratory, where it was optimally adapted to human cells and caused cryptic infections among workers who finally spread the virus causing the pandemic.

 

Cotinine-BSA Conjugate

COTN15-BSA 0.5 mg
EUR 286

Positive control tissue section for each antibody; Based on availability INQUIRE

Control-Slides Set of 5
EUR 176

Bovine Serum Albumin Biotinylated (Biotin-BSA)

BTN-BSA 5 mg
EUR 286

BSA Control for AGE-BSA

35R-AA007 10 mg
EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

CEL-BSA Control

STA-302 100 µg
EUR 322
Description:
  • N-epsilon-(carboxyethyl) lysine (CEL) modified BSA may be used as a positive control. 100 µg at 1 mg/mL in PBS.
  • Provided at 1 mg/mL in 1X PBS

MG-BSA Control

STA-306 100 µg
EUR 322
Description:
  • Methylglyoxal-BSA (MG-BSA) may be used as a positive control. 100 µg at 1 mg/mL in PBS.
  • Provided at 1 mg/mL

CML-BSA Control

STA-314 100 µg
EUR 322
Description:
  • CML-BSA may be used as a positive control. 100 µg.
  • Provided at 1 mg/mL in 1X PBS

MDA-BSA Control

STA-333 100 µg
EUR 322
Description:
  • MDA-BSA (Malondialdehyde-BSA) may be used as a positive control. 100 µg.
  • Provided at 1 mg/mL in 1X PBS

Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate

IBT15-BSA 100 ug
EUR 529

Phosphoserine-BSA control protein

PSER15-N 100 ug
EUR 164

Dinitrophenyl (DNP)-BSA protein Conjugate

AV-9330-BSA 10 mg
EUR 286

HNE antibody

20R-HG007 100 ug
EUR 300
Description: Goat polyclonal HNE antibody

mPEG-BSA (Molecular Weight: 5,000-linear)

PEG-BSA-05K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 10,000-linear)

PEG-BSA-10K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 20,000-linear)

PEG-BSA-20K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 40,000-linear)

PEG-BSA-40K 100 ug
EUR 286

4-Hydroxynonenal (HNE)-BSA protein conjugate for ELISA (in PBS)

HNE15-R 100 ug
EUR 347

(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA

DRAA31-BSA 0.5 mg
EUR 529

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA

PPPP321-BSA 0.5 mg
EUR 529

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NVDP41-BSA 0.5 mg
EUR 529

(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NANP51-BSA 0.5 mg
EUR 529

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-48T 48T
EUR 387
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-96T 96T
EUR 494
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)

SPR-211A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies 4-Hydroxynonenal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)

SPR-211B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies 4-Hydroxynonenal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)

SPR-211C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies 4-Hydroxynonenal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Protein Tyrosine Nitration Control (Nitrotyrosine-BSA)

STA-304 10 ?g
EUR 241
Description:
  • Protein Tyrosine Nitration Control (Nitrotyrosine-BSA) may be used as a positive control. 10 µg.
  • Provided at 0.1 mg/mL

Oxidized Protein Immunoblot Control (Carbonyl-BSA)

STA-309 10 ?g
EUR 241
Description:
  • Oxidized Protein Immunoblot Control (Carbonyl-BSA) may be used as a positive control. 10 µg.
  • Provided at 50 µg/mL

Monoclonal Anti-4 HNE (4-HNE; 4-hydroxynonenal) IgG

HNE13-M 100 ul
EUR 482

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column

800-302-BSA 1 Kit
EUR 347

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column

800-310-BSA 1 Kit
EUR 895

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column

800-325-BSA 1 Kit
EUR 1450

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-48Tests 48 Tests
EUR 527

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-96Tests 96 Tests
EUR 732

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-48Tests 48 Tests
EUR 551

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-96Tests 96 Tests
EUR 766

4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)

HNE11-C 100 ul
EUR 286

4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)

HNE12-C 100 ul
EUR 286

Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant

AV-1010-BSA 1 ml
EUR 286

Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit

BCA-BSA-1 1 pk
EUR 164

Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-750 1 ml
EUR 225

BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit

BSA-BGG-1 1 pk
EUR 286

4-HNE ELISA KIT|Human

EF006163 96 Tests
EUR 689

anti-4-HNE (HNEJ-2)

LF-MA90010 20 ug
EUR 360
Description: Mouse monoclonal to 4-HNE

anti-4-HNE (HNEJ-2)

LF-MA90011 100 ug
EUR 891
Description: Mouse monoclonal to 4-HNE

4-Hydroxynonenal (4-HNE) Antibody

abx411903-01ml 0.1 ml
EUR 509

Polyclonal HNE / Neutrophil Elastase Antibody

APS00008G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Sheep that recognizes and binds to Human HNE / Neutrophil Elastase . This antibody is tested and proven to work in the following applications:

Sheep 4-HNE ELISA Kit

ESH0210 96Tests
EUR 521

Rat 4-HNE ELISA Kit

ERH0210 96Tests
EUR 521

Rabbit 4-HNE ELISA Kit

ERTH0210 96Tests
EUR 521

Mouse 4-HNE ELISA Kit

EMH0210 96Tests
EUR 521

Monkey 4-HNE ELISA Kit

EMKH0210 96Tests
EUR 521

Porcine 4-HNE ELISA Kit

EPH0210 96Tests
EUR 521

Human 4-HNE ELISA Kit

EHH0210 96Tests
EUR 521

Canine 4-HNE ELISA Kit

ECH0210 96Tests
EUR 521

Chicken 4-HNE ELISA Kit

ECKH0210 96Tests
EUR 521

Bovine 4-HNE ELISA Kit

EBH0210 96Tests
EUR 521

Goat 4-HNE ELISA Kit

EGTH0210 96Tests
EUR 521

4 hydroxynonenal (HNE), >98% pure

HNE51-1 1 mg
EUR 202

4 hydroxynonenal (HNE), >98% pure

HNE51-5 5 mg
EUR 651

Pre-diluted BSA (bovine serum albumin) standards (250, 125, 62.5, 31.25, and 0.0 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-2 1 pk
EUR 164

Malondialdehyde (MDA)-BSA Conjugate positive control for Western/ELISA

MDA35-N-100 100 ug
EUR 286

4-Hydroxynonenal (4-HNE) ELISA Kit

E4645-100
EUR 865

Rat 4-Hydroxynonenal(HNE)ELISA Kit

CSB-EQ027232RA-24T 1 plate of 24 wells
EUR 165
Description: Quantitativecompetitive ELISA kit for measuring Rat 4-Hydroxynonenal (HNE) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat 4-Hydroxynonenal(HNE)ELISA Kit

1-CSB-EQ027232RA
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Rat 4-Hydroxynonenal(HNE) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse 4-Hydroxynonenal (HNE)ELISA Kit

CSB-E13412m-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse 4-Hydroxynonenal (HNE) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse 4-Hydroxynonenal (HNE)ELISA Kit

1-CSB-E13412m
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse 4-Hydroxynonenal (HNE) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human 4-Hydroxynonenal (HNE)ELISA Kit

CSB-E16214h-24T 1 plate of 24 wells
EUR 165
Description: Quantitativecompetitive ELISA kit for measuring Human 4-Hydroxynonenal (HNE) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human 4-Hydroxynonenal (HNE)ELISA Kit

1-CSB-E16214h
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Human 4-Hydroxynonenal (HNE) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

4-Hydroxynonenal (4-HNE) ELISA Kit

abx257639-96tests 96 tests
EUR 668

OxiSelect HNE Adduct Competitive ELISA Kit

STA-838 96 assays
EUR 798
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

OxiSelect HNE Adduct Competitive ELISA Kit

STA-838-5 5 x 96 assays
EUR 3240
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

Guinea Pig 4-HNE ELISA Kit

EGH0210 96Tests
EUR 521

Rabbit Anti-4 hydroxynonenal (HNE) antiserum

HNE11-S 100 ul
EUR 457

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column

800-200-BSA 1 kit
EUR 286

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column

800-205-BSA 1 kit
EUR 773

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column

800-225-BSA 1 kit
EUR 2425

Human 4-Hydroxynonenal (4-HNE) ELISA Kit

abx251411-96tests 96 tests
EUR 754

Mouse 4-Hydroxynonenal (4-HNE) ELISA Kit

abx257660-96tests 96 tests
EUR 754

Goat Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody

STA-034 100 µg
EUR 664
Description: Goat Anti-4-hydroxynonenal Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

Rabbit Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody

STA-035 100 µg
EUR 664
Description: Rabbit Anti-4-hydroxynonenal Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

ELISA kit for 4-HNE (4-Hydroxynonenal)

E-EL-0128 1 plate of 96 wells
EUR 377
Description: A competitive ELISA kit for quantitative measurement of 4-HNE (4-Hydroxynonenal) in samples from Serum, Plasma, Cell supernatant

Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit

EM1583 96T
EUR 524.1
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Human 4-HNE(4-Hydroxynonenal) ELISA Kit

EH2080 96T
EUR 524.1
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 pg/ml

Goat Anti-4 hydroxynonenal (HNE) antiserum #2

HNE12-S 100 ul
EUR 457

Rat 4-HNE(4-Hydroxynonenal) ELISA Kit

ER1587 96T
EUR 524.1
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 18.75 pg/ml

BSA

31C-CH0114 50 mg
EUR 133
Description: Bovine Serum Albumin for use in the research laboratory

Ibrutinib drug-Human Serum Albumin Conjugate

IBT16-BSA 100 ug
EUR 529

OxiSelect HNE Adduct Competitive ELISA Kit, Trial Size

STA-838-T 32 assays
EUR 421
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

Mouse (mrl/lpr) serum containing autoantibodies to HNE

HNE31-M 100 ul
EUR 482

Testosterone 3 BSA Protein (BSA)

abx060856-1mg 1 mg
EUR 578

Control siRNA Vector (pGB-control)

9500C-20
EUR 338

Histamine [G-BSA] (G-G-BSA)

DAG3327 1mg
EUR 1234

Cyclic citrullinated peptide-BSA(CCP-BSA)

E64I00802 100ug
EUR 343

4-HNE ELISA Kit| Rat 4-Hydroxynonenal ELISA Kit

EF018187 96 Tests
EUR 689

4-HNE ELISA Kit| Mouse 4-Hydroxynonenal ELISA Kit

EF014028 96 Tests
EUR 689

OxiSelect Human Oxidized LDL ELISA Kit (HNE-LDL Quantitation)

STA-389 96 assays
EUR 780
Description: LDL, so-called "bad cholesterol", is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. Oxidation of LDL affects both the lipid and protein components of LDL.  The OxiSelect Human Oxidized LDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxLDL in plasma, serum or other biological fluid samples. The kits contain a copper oxidized LDL standard against which unknown samples may be compared. This oxidized LDL assay kit is configured to selectively measure oxLDL in HNE-LDL.

OxiSelect Human Oxidized HDL ELISA Kit (HNE-HDL Quantitation)

STA-889 96 assays
EUR 780
Description: The OxiSelect Human Oxidized HDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxHDL in plasma, serum or other biological fluid samples. This oxidized HDL assay kit is configured to selectively measure HNE-modified HDL. The kit contains an HNE-HDL standard against which unknown samples may be compared.

BSA antibody

70R-AS001 500 ug
EUR 197
Description: Affinity purified Sheep polyclonal BSA antibody

Amphetamine-BSA

80-1038 1 mg
EUR 138
Description: Conjugated Amphetamine-BSA hapten

Barbiturate-BSA

80-1039 1 mg
EUR 136
Description: Conjugated Barbiturate-BSA hapten

Buprenorphine-BSA

80-1040 1 mg
EUR 136
Description: Conjugated Buprenorphine-BSA hapten

Benzodiazepine-BSA

80-1041 1 mg
EUR 138
Description: Conjugated Benzodiazepine-BSA hapten

Methamphetamine-BSA

80-1043 1 mg
EUR 138
Description: Conjugated Methamphetamine-BSA hapten

MDMA-BSA

80-1044 1 mg
EUR 138
Description: Synthethic MDMA-BSA cojugated hapten

Morphine-BSA

80-1045 1 mg
EUR 169
Description: Conjugated Morphine-BSA hapten

Methadone-BSA

80-1046 1 mg
EUR 161
Description: Conjugated Methadone-BSA hapten

Oxycodone-BSA

80-1047 1 mg
EUR 161
Description: Conjugated Oxycodone-BSA hapten

Aflatoxin-BSA

80-1049 1 mg
EUR 358
Description: Conjugated Aflatoxin-BSA hapten

THC-BSA

80-1051 1 mg
EUR 142
Description: Conjugated THC-BSA hapten

Cortisol-BSA

80-1060 500 ug
EUR 300
Description: Cortisol-BSA conjugate

Corticosterone-BSA

80-1062 500 ug
EUR 300
Description: Corticosterone-BSA conjugate

Testosterone-BSA

80-1064 500 ug
EUR 370
Description: Testosterone-BSA conjugate

Estradiol-BSA

80-1066 500 ug
EUR 349
Description: Estradiol-BSA conjugate

DHEA-BSA

80-1074 500 ug
EUR 349
Description: DHEA-BSA conjugate

Vancomycin-BSA

80-1330 1 mg
EUR 457
Description: Vancomycin-BSA Conjugated hapten for use in immunoassays

MDPV-BSA

80-1333 1 mg
EUR 392
Description: MDPV-BSA hapten conjugate

K2-BSA

80-1406 1 mg
EUR 349
Description: Conjugated K2-BSA hapten

K2-BSA

80-1407S 1 mg
EUR 457
Description: Conjugated K2-BSA hapten

Propoxyphene-BSA

80-1408 1 mg
EUR 457
Description: Conjugated Propoxyphene-BSA hapten

Fentanyl-BSA

80-1409 1 mg
EUR 349
Description: Conjugated Fentanyl-BSA hapten

Serotonin-BSA

80-1416 1 mg
EUR 651
Description: BSA conjugated Serotonin Hapten

Ciprofloxacin-BSA

80-1428 1 mg
EUR 651
Description: BSA conjugated Ciprofloxacin Hapten

Clenbuterol-BSA

80-1430 1 mg
EUR 349
Description: BSA conjugated Clenbuterol Hapten

Corticosterone-BSA

80-1432 1 mg
EUR 651
Description: BSA conjugated Corticosterone Hapten

Cortisol-BSA

80-1434 1 mg
EUR 651
Description: BSA conjugated Cortisol Hapten

DHEA-BSA

80-1436 1 mg
EUR 651
Description: BSA conjugated DHEA Hapten

DHEA-BSA

80-1437 1 mg
EUR 651
Description: BSA conjugated DHEA Hapten

Dopamine-BSA

80-1442 1 mg
EUR 349
Description: BSA conjugated Dopamine Hapten

Enrofloxacin-BSA

80-1444 1 mg
EUR 651
Description: BSA conjugated Enrofloxacin Hapten

Estradiol-BSA

80-1446 1 mg
EUR 651
Description: BSA conjugated Estradiol Hapten

Estrone-BSA

80-1448 1 mg
EUR 651
Description: BSA conjugated Estrone Hapten

Gentamicin-BSA

80-1453 1 mg
EUR 651
Description: BSA conjugated Gentamicin Hapten

Gentamicin-BSA

80-1454 1 mg
EUR 651
Description: BSA conjugated Gentamicin Hapten

Gibberellin-BSA

80-1457 1 mg
EUR 349
Description: BSA conjugated Gibberellin Hapten

Histamine-BSA

80-1460 1 mg
EUR 349
Description: BSA conjugated Histamine Hapten

IAA-BSA

80-1464 1 mg
EUR 349
Description: BSA conjugated IAA Hapten

IAA-BSA

80-1465 1 mg
EUR 349
Description: BSA conjugated IAA Hapten

Kanamycin-BSA

80-1467 1 mg
EUR 651
Description: BSA conjugated Kanamycin Hapten

Melatonin-BSA

80-1469 1 mg
EUR 651
Description: BSA conjugated Melatonin Hapten

Neomycin-BSA

80-1471 1 mg
EUR 651
Description: BSA conjugated Neomycin Hapten

Ofloxacin-BSA

80-1477 1 mg
EUR 651
Description: BSA conjugated Ofloxacin Hapten

Ractopamine-BSA

80-1479 1 mg
EUR 651
Description: BSA conjugated Ractopamine Hapten

Glutathione-BSA

80-1481 1 mg
EUR 651
Description: BSA conjugated Glutathione Hapten

SAH-BSA

80-1486 1 mg
EUR 651
Description: BSA conjugated SAH Hapten

Salbutamol-BSA

80-1489 1 mg
EUR 349
Description: BSA conjugated Salbutamol Hapten

Streptomycin-BSA

80-1492 1 mg
EUR 651
Description: BSA conjugated Streptomycin Hapten

Sulfadiazine-BSA

80-1496 1 mg
EUR 651
Description: BSA conjugated Sulfadiazine Hapten

Sulfadimidine-BSA

80-1500 1 mg
EUR 651
Description: BSA conjugated Sulfadimidine Hapten

Sulfamethoxazole-BSA

80-1504 1 mg
EUR 651
Description: BSA conjugated Sulfamethoxazole Hapten

Sulfamethoxazole-BSA

80-1505 1 mg
EUR 651
Description: BSA conjugated Sulfamethoxazole Hapten

Sulfamethoxydiazine-BSA

80-1508 1 mg
EUR 651
Description: BSA conjugated Sulfamethoxydiazine Hapten

Sulfamonomethoxine-BSA

80-1510 1 mg
EUR 651
Description: BSA conjugated Sulfamonomethoxine Hapten

Sulfamonomethoxine-BSA

80-1511 1 mg
EUR 651
Description: BSA conjugated Sulfamonomethoxine Hapten

Testosterone-BSA

80-1516 1 mg
EUR 651
Description: BSA conjugated Testosterone Hapten

Furazolidone-BSA

80-1518 1 mg
EUR 651
Description: BSA conjugated Furazolidone Hapten

Nitrofurantoin-BSA

80-1520 1 mg
EUR 651
Description: BSA conjugated Nitrofurantoin Hapten

Nitrofurazone-BSA

80-1522 1 mg
EUR 651
Description: BSA conjugated Nitrofurazone Hapten

Thyroxine-BSA

80-1524S 1 mg
EUR 349
Description: BSA conjugated Thyroxine Hapten

GABA-BSA

80-1529 1 mg
EUR 349
Description: BSA conjugated GABA Hapten

Cotinine-BSA

80-1594 1 mg
EUR 367
Description: Conjugated Cotinine-BSA

Amphetamine-BSA

80-IA22 1 mg
EUR 227
Description: Conjugated Amphetamine-BSA hapten

Amphetamine-BSA

80-IA23 1 mg
EUR 227
Description: Conjugated Amphetamine-BSA hapten

Amphetamine-BSA

80-IA24 1 mg
EUR 219
Description: Conjugated Amphetamine-BSA hapten

Barbital-BSA

80-IB21 1 mg
EUR 179
Description: Conjugated Barbital-BSA hapten

Benzoylecgonine-BSA

80-IB29 1 mg
EUR 227
Description: Conjugated Benzoylecgonine-BSA hapten

Benzoylecgonine-BSA

80-IB31 1 mg
EUR 227
Description: Conjugated Benzoylecgonine-BSA hapten

Benzodiazepine-BSA

80-IB40 1 mg
EUR 218
Description: Conjugated Benzodiazepine-BSA hapten

Benzodiazepine-BSA

80-IB42 1 mg
EUR 219
Description: Conjugated Benzodiazepine-BSA hapten

Digoxin-BSA

80-ID10 10 mg
EUR 279
Description: Conjugated Digoxin-BSA hapten

Gentamicin-BSA

80-IG10 25 mg
EUR 381
Description: Conjugated Gentamicin-BSA hapten

Methadone-BSA

80-IM40 1 mg
EUR 219
Description: Conjugated Methadone-BSA hapten

Morphine-BSA

80-IM50 1 mg
EUR 227
Description: Conjugated Morphine-BSA hapten

Morphine-BSA

80-IM52 1 mg
EUR 227
Description: Conjugated Morphine-BSA hapten

Morphine-BSA

80-IM53 1 mg
EUR 138
Description: Conjugated Morphine-BSA hapten

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