fondationvocation

van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel

Kwantitatieve bepaling van sporenmetaal in celkweekmedia met behulp van LS-APGD-MS en ICP-OES met differentiatie van vrije / gebonden soorten na scheidingen van polymeervezels

Liquid sampling-atmospheric pressure glow discharge-mass spectrometry (LS-APGD-MS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES) were employed for the quantification of trace metals in cell culture media and their capabilities compared. The LS-APGD is interfaced here to a compact mass spectrometer (Advion CMS) towards the development of an at-bioreactor process monitoring strategy. Both techniques have been previously employed for the quantification of trace metals in samples of various complexities, making them a natural choice for this application.

They have also demonstrated comparable analytical figures of merit including limits of detection (LOD), matrix tolerance, etc. While cell culture media is a complex sample, the ICP-OES technique was unaffected by the matrix. However, the LS-APGD-MS suffered from increases in spectral background. Despite this, both techniques achieved appropriate LODs for all metals analyzed in this work (Cu, Fe, Zn, Co, Mn, Ni; LOD < 100 ng mL-1), except for Mn and Ni via LS-APGD-MS. To overcome the increased background seen on the LS-APGD-MS, a capillary channeled polymer (C-CP) polypropylene (PPY) fiber stationary phase was employed as an on-line separation for the removal of organic components prior to sample introduction into the plasma.
It was further determined that Ni was retained on the column, preventing the detection of this element via LS-APGD-MS, and insights into metal speciation were discussed. Following implementation of this on-line separation strategy, the agreement between the techniques was acceptable for all analytes, and was excellent for Cu, Fe, and Zn.

Co-gereguleerde pilus van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel van Caco-2 en perifere bloedmononucleaire cellen (PBMC), wat leidt tot verhoogde kolonisatie

The aim of this study was to assess the modulatory effect of TcpA in the expression of CEACAM1 adhesin molecule and IL-1, IL-8 and TNFα pro-inflammatiory cytokines in the co-culture model of Caco-2/PBMC that can mimic the intestinal milieu. The tcpA gene from V.cholerae ATCC14035 was cloned in pET-28a and transformed into E.coli Bl-21. The recombinant TcpA-His6 protein was expressed and purified using the Ni-column chromatography.
The sequencing of transformed plasmid and Western blotting of purified protein confirmed the identity of rTcp. Cytotoxicity of different concentrations of recombinant protein for human colon carcinoma cell line (Caco-2 cell) was assessed by MTT assay and showed viability of 92%, 82% and 70 %, for10 µg/mL of TcpA after 24, 48 and 72 h, respectively. Co-cultures of Caco-2 and peripheral blood mononuclear cells (PBMCs) were used to mimic the intestinal milieu and treated with different concentrations of rTcpA (1, 5, 10 and 50 µg/mL).
Our data showed about 2.04, 3.37, 3.68 and 42.7-folds increase in CEACAM1 gene expression, respectively, compared with non-treated Caco-2/PBMC co-culture. Moreover, the expression of IL-1, IL-8 and TNFα genes was significantly increased up to 15.75, 7.04 and 80.95 folds, respectively. In conclusion, V.cholerae TcpA induces statistically significant dose-dependent stimulatory effect on TNFα, IL-1 and IL-8 pro-inflammatory cytokines expression. Among all, TNFα was much more affected which consequently, elevated CEACAM1 expression level in IECs. This suggests TcpA protein as critical effector as an inducer of increased adhesion potential of V. cholera as well as inflammatory responses of host intestinal tissue. This article is protected by copyright. All rights reserved.
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pAAV-RC2 Vector

MBS169538-5x001mg 5x0.01mg
EUR 2955

pAAV-RC2

PVT35966-1 2ug
EUR 280

pAAV-RC1 Vector

VPK-421 10 µg
EUR 530

pAAV-RC3 Vector

VPK-423 10 µg
EUR 530

pAAV-RC4 Vector

VPK-424 10 µg
EUR 530

pAAV-RC5 Vector

VPK-425 10 µg
EUR 530

pAAV-RC6 Vector

VPK-426 10 µg
EUR 530

pAAV-RC1 Vector

MBS169537-001mg 0.01mg
EUR 695

pAAV-RC1 Vector

MBS169537-5x001mg 5x0.01mg
EUR 2955

pAAV-RC3 Vector

MBS169539-001mg 0.01mg
EUR 695

pAAV-RC3 Vector

MBS169539-5x001mg 5x0.01mg
EUR 2955

pAAV-RC4 Vector

MBS169540-001mg 0.01mg
EUR 695

pAAV-RC4 Vector

MBS169540-5x001mg 5x0.01mg
EUR 2955

pAAV-RC5 Vector

MBS169541-001mg 0.01mg
EUR 695

pAAV-RC5 Vector

MBS169541-5x001mg 5x0.01mg
EUR 2955

pAAV-RC6 Vector

MBS169542-001mg 0.01mg
EUR 695

pAAV-RC6 Vector

MBS169542-5x001mg 5x0.01mg
EUR 2955

pAAV-RC9 vector

PVT12073 2ug
EUR 408

pAAV-DJ Vector

VPK-420-DJ 10 µg
EUR 530

pAAV-DJ Vector

MBS169535-001mg 0.01mg
EUR 695

pAAV-DJ Vector

MBS169535-5x001mg 5x0.01mg
EUR 2955

pAAV-DJ vector

PVT12151 2ug
EUR 325

pAAV-DJ/8 Vector

VPK-420-DJ-8 10 µg
EUR 530

pAAV-DJ/8 Vector

MBS169536-001mg 0.01mg
EUR 695

pAAV-DJ/8 Vector

MBS169536-5x001mg 5x0.01mg
EUR 2955

pAAV-GFP Control Vector

AAV-400 10 µg
EUR 455

pAAV-Cre Control Vector

AAV-401 10 µg
EUR 679.2
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-GFP Control Vector

MBS168881-001mg 0.01mg
EUR 610

pAAV-GFP Control Vector

MBS168881-5x001mg 5x0.01mg
EUR 2575

pAAV-LacZ Control Vector

AAV-402 10 µg
EUR 679.2
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 530

pAAV-MCS Expression Vector

MBS169528-001mg 0.01mg
EUR 695

pAAV-MCS Expression Vector

MBS169528-5x001mg 5x0.01mg
EUR 2955

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 530

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

MBS169533-001mg 0.01mg
EUR 695

pAAV-IRES-GFP Expression Vector

MBS169533-5x001mg 5x0.01mg
EUR 2955

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 530

pAAV-MCS Promoterless Expression Vector

MBS169529-001mg 0.01mg
EUR 695

pAAV-MCS Promoterless Expression Vector

MBS169529-5x001mg 5x0.01mg
EUR 2955

Green Kit. Baculovirus GFP vector.

K20 1 Kit
EUR 695
Description: Protein expression

ProGreen. Baculovirus GFP marker vector.

A1 25 ul
EUR 420
Description: Protein expression

pVL1393. General baculovirus plasmid vector.

B1 50 ul
EUR 340
Description: Protein expression

ProEasy. Vector for easy construction of recombinant baculoviruses.

A10S 25 ul
EUR 695
Description: Protein expression

pAcAB3. Baculovirus plasmid vector for expression of up to 3 proteins.

B2 50 ul
EUR 420
Description: Protein expression

pAB-bee. Baculovirus plasmid vector for secreted and transmembrane proteins.

B3 50 ul
EUR 495
Description: Protein expression

ProFold-PDI. Baculovirus chaperone vector for expression of cysteine-rich proteins.

A7 25 ul
EUR 830
Description: Protein expression

ProFold-C1. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins.

A2 25 ul
EUR 830
Description: Protein expression

ProFold-C2. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins.

A3 25 ul
EUR 830
Description: Protein expression

ProFold-ER1. Baculovirus chaperone vector for expression of secreted and membrane proteins.

A4 25 ul
EUR 830
Description: Protein expression

pAAV-RC6

PVT14647 2ug
EUR 580

C1 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins.

K21 1 Kit
EUR 995
Description: Protein expression

C2 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins.

K22 1 Kit
EUR 995
Description: Protein expression

ER1 Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins.

K23 1 Kit
EUR 995
Description: Protein expression

ER1-bee Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins.

K24 1 Kit
EUR 995
Description: Protein expression

pAAV-RC

PVTY00744 2ug
EUR 280

pAAV- RC

PVT2103 2ug
EUR 162

pAAV- MCS

PVT2102 2ug
EUR 162

pAAV-MCS

PVTY00902 2ug
EUR 280

pAAV-GFP

PVT23853 2ug
EUR 280

pAAV-LacZ

PVTY00602 2ug
EUR 280

pAAV-hrGFP

PVTY00600 2ug
EUR 280

pAAV-CLDN5

PVT28405 2ug
EUR 280

pAAV-DNMT3A

PVT28396 2ug
EUR 280

pAAV-Smc6-m

PVT20174 2ug
EUR 280

pAAV-CAG-RFP

PVTY00160 2ug
EUR 280

pAAV-CAG-GFP

PVT17666 2ug
EUR 232

pAAV-fNPY-GFP

PVT14636 2ug
EUR 480

pAAV-IRES-EGFP

PVTY00099 2ug
EUR 280

pAAV-tTS-shRNA

PVTY00265 2ug
EUR 280

pAAV- IRES- ZsGreen1

PVT11044 2ug
EUR 215

pAAV-hSyn-EGFP

PVT22104 2ug
EUR 280

pAAV-NMNAT1(-FLAG

PVT25866 2ug
EUR 280

pAAV-NMNAT3(-FLAG

PVT25867 2ug
EUR 280

pAAV-IRES-hrGFP

PVTY00601 2ug
EUR 280

pAAV- ZsGreen1- shRNA

PVT11045 2ug
EUR 260

pAAV-hSyn-PDE7B

PVT30915 2ug
EUR 280

pAAV-RSV-SpCas9

PVT17629 2ug
EUR 198

pAAV-TagRFP-P4M

PVT19979 2ug
EUR 280

pAAV-hSyn-PDE7B

PVT30451 2ug
EUR 280

pAAV-hSyn-PDE7A

PVT30596 2ug
EUR 280

pAAV-hSyn-PDE7A

PVT30619 2ug
EUR 280

pAAV-CMV-AK080112

PVT31829 2ug
EUR 280

pAAV-Abcb1a Plasmid

PVT44568 2ug
EUR 280

pAAV-TRE3-GCaMp7f

PT-6929 1 vial Ask for price
Description: Adeno-associated virus vector.

pAAV-IRES-tdTomato

PVTY00132 2ug
EUR 280

pAAV-IRES-ZsGreen1

PVTY00133 2ug
EUR 280

pAAV-ZsGreen-miRNA

PVTY00266 2ug
EUR 280

pAAV-ZsGreen1-shRNA

PVTY00157 2ug
EUR 280

pAAV-tdTomato-shRNA

PVTY00158 2ug
EUR 280

pAAV-ZsGreen1-shRNA

PVT26010 2ug
EUR 280

pAAV- IRES- hrGFP Plasmid

PVT2104 2ug
EUR 182

pAAV-CMV-Cas9C-VPR

PVT32936 2ug
EUR 280

pAAV-cTNT-Cre Plasmid

PVT50562 2ug
EUR 280

pAAV-SMC1A-FLAG Plasmid

PVT41602 2ug
EUR 280

pAAV-EF1a-DIO-mCherry

PVT17841 2ug
EUR 198

pAAV-NR4A2-IRES-hrGFP

PVT30138 2ug
EUR 280

pAAV-EGFP-Steap3 Plasmid

PVT49392 2ug
EUR 280

pAAV-CasRx-PregRNA-WPRE

PVT34684 2ug
EUR 280

pAAV- CMV- mCherry- U6- sgRNA

PVT11046 2ug
EUR 215

pAAV-hSyn-eNpHR 3.0-EYFP

PVT19063 2ug
EUR 162

pAAV-CMV-NGF-T2A-EGFP

PVT20159 2ug
EUR 280

pAAV-U6-sgRNA-CMV-GFP

PVT23304 2ug
EUR 280

pAAV-CMV-EGFP-MCS Plasmid

PVT50482 2ug
EUR 280

pAAV-EnCMV-MCS-U6-shRNA

PVT37376 2ug
EUR 280

pAAV-CMV-FLAG-MCS Plasmid

PVT47683 2ug
EUR 280

pAAV-CMV-mCherry-P2A-MCS

PVT36620 2ug
EUR 280

pAAV-CAG-FLEX-EGFP Plasmid

PVT46535 2ug
EUR 280

pAAV-CMV-EGFP-LC3B Plasmid

PVT47550 2ug
EUR 280

pAAV-MCS-mCherry-T2A-Luc

PVT24390 2ug
EUR 280

pAAV-pCD68-MCS-EGFP Plasmid

PVT49285 2ug
EUR 280

pAAV-CMV-EGFP-Cpxm2 Plasmid

PVT51039 2ug
EUR 280

pAAV-CAG-Aldh2-EGFP Plasmid

PVT51087 2ug
EUR 280

pAAV-CAG-Acss3-EGFP Plasmid

PVT51333 2ug
EUR 280

pAAV-hSyn-hChR2(H134R)-mCherry

PVT19026 2ug
EUR 162

pAAV-CMV-VEGFA-T2A-mKate2

PVT20158 2ug
EUR 280

pAAV-CMV-eNpHR-EYFP Plasmid

PVT46330 2ug
EUR 280

pAAV-CAG-EGFP-Syvn1 Plasmid

PVT48299 2ug
EUR 280

pAAV-pCD68-Olr1-EGFP Plasmid

PVT49273 2ug
EUR 280

pAAV-EF1a-AGER-DIO-mCherry

PVT22028 2ug
EUR 280

pAAV-hSyn-jRGECO1a-Xc-WPRE

PT-6283 1 vial Ask for price
Description: Adeno-associated virus vector.

pAAV-pCD68-Klf15-EGFP Plasmid

PVT49379 2ug
EUR 280

pAAV-CMV-Aldh2-ZsGreen1 Plasmid

PVT50269 2ug
EUR 280

pAAV-CMV-Acss3-ZsGreen1 Plasmid

PVT50337 2ug
EUR 280

pAAV-TagRFP-P4M-Pou4f3 promoter

PVT20081 2ug
EUR 280

pAAV-CMV-Lhcgr-ZsGreen1 Plasmid

PVT48346 2ug
EUR 280

pAAV-CMV-Fgf21-Zsgreen1 Plasmid

PVT48402 2ug
EUR 280

pAAV-CamKII-3×HA-Klc1 Plasmid

PVT48763 2ug
EUR 280

pAAV-EF1a-DIO-hM3D(Gq)-mCherry

PVT17847 2ug
EUR 198

pAAV-CamKII-Pdk2-2×HA Plasmid

PVT46920 2ug
EUR 280

pAAV-CamKII-Pdk1-2×HA Plasmid

PVT46927 2ug
EUR 280

pAAV-CamKII-Pdpr-2×HA Plasmid

PVT46942 2ug
EUR 280

pAAV-CamKII-Pdp2-2×HA Plasmid

PVT46945 2ug
EUR 280

pAAV-CamKII-Pdk3-2×HA Plasmid

PVT46956 2ug
EUR 280

pAAV-CamKII-Pdk4-2×HA Plasmid

PVT46960 2ug
EUR 280

pAAV-CamKII-Pdp1-2×HA Plasmid

PVT47084 2ug
EUR 280

pAAV-CamKII-Nme2-3×FLAG Plasmid

PVT48775 2ug
EUR 280

pAAV-CamKII-Nme1-3×FLAG Plasmid

PVT48956 2ug
EUR 280

pAAV-CamKII-Lhpp-3×FLAG Plasmid

PVT48961 2ug
EUR 280

pAAV-MCS-Ppargc1a-m-FLAG-HA

PVT18341 2ug
EUR 198

pAAV-CamKII-3×FLAG-Phpt1 Plasmid

PVT48951 2ug
EUR 280

pAAV-CamKII-Pgam5-3×FLAG Plasmid

PVT49667 2ug
EUR 280

pAAV-U6-sgRNA-EnCMV-Cre-WPRE

PVT34683 2ug
EUR 280

pAAV-EFs-CasRx-U6-gRNA1-gRNA2

PVT28511 2ug
EUR 280

pAAV-EnCMV-MCS-U6-MAPT-shRNA1

PVT37609 2ug
EUR 280

pAAV-cTNT-CasRx-U6-gRNA1-gRNA2

PVT33708 2ug
EUR 280

pAAV-CMV-hChR2(H134R)-mCherry Plasmid

PVT45728 2ug
EUR 280

pAAV-CAG-EGFP-CMV-mCherry Plasmid

PVT48627 2ug
EUR 280

pAAV-CMV-mCherry-P2A-PIAS1-FLAG

PVT36938 2ug
EUR 280

pAAV-U6-MCS-shRNA-ZsGreen1 Plasmid

PVT50749 2ug
EUR 280

pAAV-CMV-Runx1-T2A-ZsGreen1 Plasmid

PVT50702 2ug
EUR 280

pAAV-ZsGreen1-U6-Acss2-shRNA3 Plasmid

PVT49600 2ug
EUR 280

pAAV-U6-Acss3-shRNA2-ZsGreen1 Plasmid

PVT49730 2ug
EUR 280

pAAV-U6-Acss3-shRNA1-ZsGreen1 Plasmid

PVT49735 2ug
EUR 280

pAAV-ZsGreen1-U6-Acss2-shRNA2 Plasmid

PVT49810 2ug
EUR 280

pAAV-ZsGreen1-U6-Acss1-shRNA1 Plasmid

PVT49886 2ug
EUR 280

pAAV-ZsGreen1-U6-Acss1-shRNA2 Plasmid

PVT50084 2ug
EUR 280

pAAV-U6-Pparg-shRNA1-ZsGreen1 Plasmid

PVT50229 2ug
EUR 280

pAAV-U6-Pparg-shRNA2-ZsGreen1 Plasmid

PVT50231 2ug
EUR 280

pAAV-U6-Pparg-shRNA3-ZsGreen1 Plasmid

PVT50372 2ug
EUR 280

pAAV-U6-Slc29a1-shRNA1-ZsGreen1 Plasmid

PVT51375 2ug
EUR 280

pAAV-RORE-Promoter-Fluc-mCherry Plasmid

PVT47364 2ug
EUR 280

Een nieuwe isolatie- en kweekmethode voor granulosacellen

Granulosa cells are the cell population who have an increasing importance in the female genital system and reproduction. Thus, nowadays in vitro studies to address these cells are also gaining importance and attracts researcher’s attention. The aim of our study is to develop a more feasible, low-cost granulosa cell isolation and culture method compared to methods defined so far.
Granulosa cells were isolated from follicular fluids obtained from both healthy women donors (n = 19) and polycystic ovary syndrome (n = 15) applied to in vitro fertilization treatment process. Granulosa cells were isolated by using Lymphosep® separation fluid that was not used for this purpose before. The isolated cells were cultured in suitable culture dishes with a mixture of BIO-AMFTM-1 and DMEM/F12 in the first seeding and only complete DMEM/F12 in the following feeds.
  • The complete medium contains only 5% fetal calf serum, 4% L-glutamine and 1% penicillin-streptomycin-amphotericin. The new methods we have developed in granulosa cell isolation and in vitro culture have been successful.
  • Reduction in supplement types and amount; improved the proliferation rate of the granulosa cells in culture. Our new methods of isolation and cell culture for granulosa cells from healthy women, have been also successful in samples of polycystic ovarian patients.
  • With these developed methods, granulosa cells, which belong to humans and have an important role in the ovary, could be isolated and subsequently be maintained to reproduce (proliferate) more easily and cheaper.

Over de oorsprong van SARS-CoV-2: hebben celkweekexperimenten geleid tot verhoogde virulentie van het voorlopervirus voor mensen?

We are currently in a rapidly expanding pandemic of the SARS-CoV-2 virus, which originated in the city of Wuhan in central China. The disease COVID-19 is now spread worldwide and has tremendous socio-economic consequences. The origin of the virus can be reconstructed through epidemiological studies and, even more so, from genome comparisons. How the evolution of the virus and the transition to humans might have happened is the subject of much speculation.
It is considered certain that the virus is of animal origin and very likely passed from bats to humans in a zoonotic event. An intermediate host was postulated, but many SARS-like bat viruses have the ability to infect human cells directly, which has been shown experimentally by scientists in the Wuhan Institute of Virology using collected specimens containing virus material from horseshoe bats.
The propagation of SARS-like bat viruses in cell culture allowed experiments aimed at increasing the infectivity of the virus and adaptation to human cells. This article summarizes the unique properties of SARS-CoV-2 and focusses on a specific sequence encoding the spike protein.
Possible scenarios of virus evolution are discussed, with particular emphasis on the hypothesis that the virus could have emerged unintentionally through routine culture or gain-of-function experiments in a laboratory, where it was optimally adapted to human cells and caused cryptic infections among workers who finally spread the virus causing the pandemic.

 

HNE-BSA Control

MBS168152-5x01mg 5x0.1mg
EUR 1450

HNE (4-Hydroxy-2-nonenal), ELISA Control (BSA)

MBS656950-01mg 0.1mg
EUR 715

HNE (4-Hydroxy-2-nonenal), ELISA Control (BSA)

MBS656950-5x01mg 5x0.1mg
EUR 3075

HNE (4-Hydroxy-2-nonenal), Western Blot Control (BSA)

MBS656952-01mL 0.1mL
EUR 575

HNE (4-Hydroxy-2-nonenal), Western Blot Control (BSA)

MBS656952-5x01mL 5x0.1mL
EUR 2440

BSA Control for Age-BSA

2221-BSA each
EUR 210

BSA Control for AGE-BSA

35R-AA007 10 mg Ask for price
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

BSA Control for AGE protein (BSA modified)

MBS5310912-10mg 10mg
EUR 290

BSA Control for AGE protein (BSA modified)

MBS5310912-5x10mg 5x10mg
EUR 1205

MG-BSA Control

STA-306 100 µg
EUR 386.4
Description:
  • Methylglyoxal-BSA (MG-BSA) may be used as a positive control. 100 µg at 1 mg/mL in PBS.
  • Provided at 1 mg/mL

CEL-BSA Control

STA-302 100 µg
EUR 245

CML-BSA Control

STA-314 100 µg
EUR 386.4
Description:
  • CML-BSA may be used as a positive control. 100 µg.
  • Provided at 1 mg/mL in 1X PBS

MDA-BSA Control

STA-333 100 µg
EUR 245

CEL-BSA Control

MBS168667-01mg 0.1mg
EUR 360

CEL-BSA Control

MBS168667-5x01mg 5x0.1mg
EUR 1450

MDA-BSA Control

MBS168718-01mg 0.1mg
EUR 360

MDA-BSA Control

MBS168718-5x01mg 5x0.1mg
EUR 1450

Phosphoserine-BSA control protein

PSER15-N 100 ug
EUR 196.8

Malondialdehyde (MDA)-BSA Conjugate positive control for Western/ELISA

MDA35-N-100 100 ug
EUR 343.2

HNE (4-Hydroxy-2-nonenal) (BSA)

MBS656913-05mg 0.5mg
EUR 630

HNE (4-Hydroxy-2-nonenal) (BSA)

MBS656913-5x05mg 5x0.5mg
EUR 2680

Oxidized Protein Immunoblot Control (Carbonyl-BSA)

STA-309 10 ?g
EUR 170

Oxidized Protein Immunoblot Control (Carbonyl-BSA)

MBS168896-001mg 0.01mg
EUR 285

Oxidized Protein Immunoblot Control (Carbonyl-BSA)

MBS168896-5x001mg 5x0.01mg
EUR 1120

Protein Tyrosine Nitration Control (Nitrotyrosine-BSA)

STA-304 10 ?g
EUR 289.2
Description:
  • Protein Tyrosine Nitration Control (Nitrotyrosine-BSA) may be used as a positive control. 10 µg.
  • Provided at 0.1 mg/mL

Rabbit Anti-Bovine Serum Albumin (BSA) IgG positive control (50-75 U/ml, 1 ml)

710-113-PC 1
EUR 196.8

Isotype Control, Mouse IgG1 Kappa (IGG1/1331), Purified, with BSA, 0.2 mg/mL

BNUB1331-250 1uL
EUR 57
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Isotype Control, Mouse IgG2b Kappa (IgG2b/1333), Purified, with BSA, 0.2 mg/mL

BNUB1333-250 1uL
EUR 57
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Isotype Control, Mouse IgG1 Kappa (IGG1/1331), Purified, BSA-free, 1 mg/mL

BNUM1331-50 1uL
EUR 63
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

4-Hydroxynonenal (HNE)-BSA protein conjugate for ELISA (in PBS)

HNE15-R 100 ug
EUR 416.4

Isotype Control, Mouse IgG2a Kappa (MG2a-53), Purified, with BSA, 0.2 mg/mL

BNUB1331B-250 1uL
EUR 57
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Isotype Control, Mouse IgG2b Kappa (IgG2b/1333), Purified, BSA-free, 1 mg/mL

BNUM1333-50 1uL
EUR 63
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Isotype Control, Mouse IgG2a Kappa (MG2a-53), Purified, BSA-free, 1 mg/mL

BNUM1331B-50 1uL
EUR 63
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Rabbit Anti-Bovine Serum Albumin (BSA) IgG negative control (0 U/ml, 1 ml)

710-113-NC 1 ml
EUR 123.6

Hydroxynonenal modified Bovine Serum Albumin (HNE-BSA)

MBS390130-02mg 0.2mg
EUR 355

Hydroxynonenal modified Bovine Serum Albumin (HNE-BSA)

MBS390130-05mg 0.5mg
EUR 630

Hydroxynonenal modified Bovine Serum Albumin (HNE-BSA)

MBS390130-1mg 1mg
EUR 955

Hydroxynonenal modified Bovine Serum Albumin (HNE-BSA)

MBS390130-5x1mg 5x1mg
EUR 4220

4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)

HNE11-C 100 ul
EUR 343.2

4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)

HNE12-C 100 ul
EUR 343.2

Rat neurofascin (Nfasc)-control Control/blocking peptide

AB-23249-CP 100ug
EUR 196.8

AS Control

A26 -
EUR 330
Description: SW module for AS control

GC Control

A23 -
EUR 600
Description: SW module for GC control

LC Control

A24 -
EUR 600
Description: SW module for HPLC/CE control (pump, det, etc.)

Rat Control

DEV99RC 2 x 1 ml
EUR 60

MDP control

tlrl-mdpcl 5 mg
EUR 148.05

BMP4 Control

MBS542571-5Applications 5Applications
EUR 335

BMP4 Control

MBS542571-5x5Applications 5x5Applications
EUR 1350

Rat IgG-PE conjugate (isotype control) (Isotype control)

20005-PE 25 tests
EUR 242.4

Rat IgG-HRP conjugate (isotype control) (Isotype control)

20005-HP 100 ug
EUR 196.8

NPY1R Control

MBS543000-5Applications 5Applications
EUR 335

ADCY3 Control

MBS543023-5Applications 5Applications
EUR 335

ADCY3 Control

MBS543023-5x5Applications 5x5Applications
EUR 1350

NPY1R Control

MBS543000-5x5Applications 5x5Applications
EUR 1350

pGIPZ control

PVTY00348 2ug
EUR 280

pMD19- Control

PVT10564 2ug
EUR 182

Rat IgG-FITC conjugate (isotype control) (Isotype control)

20005-F 100 ug
EUR 196.8

Control Slides

24012-1 1box
EUR 78

Canine Control

DEV99CC 2 x 1 ml
EUR 60

Dengue Control

ant-543 100µg
EUR 165
Description: Mouse Anti-Dengue Control for Lateral Flow Test

Canine Control

MBS495203-1Set 1Set
EUR 305

Canine Control

MBS495203-5x1Set 5x1Set
EUR 1390

pTRIPZ control

PVTY00369 2ug
EUR 280

Rat IgG-Biotin conjugate (isotype control) (Isotype control)

20005-B 100 ug
EUR 196.8

Quality Control

abx098966-1vial 1 vial
EUR 360

Ascites, Control

MBS652640-10mL 10mL
EUR 415

Ascites, Control

MBS652640-1mL 1mL
EUR 190

Ascites, Control

MBS652640-5x10mL 5x10mL
EUR 1710

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 100 test
EUR 123.6

Control for Tyrosinase, 4 cases (1.5mm)HumanskinSkinIHC control

TYROSIN081 1.5mm
EUR 50

Negative Control

MBS2568128-1mL 1mL
EUR 140

Negative Control

MBS2568129-1mL 1mL
EUR 135

Positive Control

MBS2568130-1mL 1mL
EUR 150

Positive Control

MBS2568131-1mL 1mL
EUR 150

PIPET CONTROLLER CONTROL BOARD

357484 1/pk
EUR 104.4
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories

Control siRNA Vector (pGB-control)

9500C-20 each
EUR 405.6

OOSA10387-100T - IgM NEGATIVE CONTROL Negative/Isotype Control

OOSA10387-100T 100Tests
EUR 309

OOSA10342-100UG - IgG1 NEGATIVE CONTROL Negative/Isotype Control

OOSA10342-100UG 0.1mg
EUR 219

OOSA10349-100T - IgG2a NEGATIVE CONTROL Negative/Isotype Control

OOSA10349-100T 100Tests
EUR 299

OOSA10380-100T - IgG2b NEGATIVE CONTROL Negative/Isotype Control

OOSA10380-100T 100Tests
EUR 309

OOSA10389-100T - IgG1 NEGATIVE CONTROL Negative/Isotype Control

OOSA10389-100T 100Tests
EUR 309

OOSA10397-100T - IgG2a NEGATIVE CONTROL Negative/Isotype Control

OOSA10397-100T 100Tests
EUR 309

CypExpress Control

MBS480553-10g 10g
EUR 1905

CypExpress Control

MBS480553-1g 1g
EUR 320

CypExpress Control

MBS480553-5x10g 5x10g
EUR 8355

CypExpress Control

MBS480553-5x1g 5x1g
EUR 1220

ODN 2088 control

tlrl-2088c-1 1 mg
EUR 546

ODN 2395 control

tlrl-2395c-1 1 mg
EUR 546

Control for Mammaglobin, 4 cases (1.5mm)HumanBreastBreastIHC control

MAMA-G081 1.5mm
EUR 50

SERION ELISA Avidity control Rubella Virus Avidity Control IgG

MBS191655-INQUIRE INQUIRE Ask for price

Control FD BNP

K023M-6 6 x 1 mL
EUR 292
Description: Bi-Level

Control FD TDM

K068M-6 6 x 5 mL
EUR 205
Description: Tri-Level

Control for GCDFP-15, 4 cases (1.5mm)HumanBreastBreastIHC control

GCDFP081 1.5mm
EUR 50

EDG-1 Control

BC-009 100 uL
EUR 350

EDG-2 Control

BC-011 100 uL
EUR 350

EDG-4 Control

BC-012 100 uL
EUR 350

EDG-6 Control

BC-014 100 uL
EUR 350

EDG-7 Control

BC-016 100 uL
EUR 350

Rat Control IgG

AC034 100 ul
EUR 159.6

β-Gal Control

LV007 4 x 500 ul
EUR 195

NF-κB Control

T37559-10mg 10mg Ask for price
Description: NF-κB Control

NF-κB Control

T37559-1g 1g Ask for price
Description: NF-κB Control

NF-κB Control

T37559-1mg 1mg Ask for price
Description: NF-κB Control

NF-κB Control

T37559-50mg 50mg Ask for price
Description: NF-κB Control

NF-κB Control

T37559-5mg 5mg Ask for price
Description: NF-κB Control

Rat Control IgG

MBS9144358-01mL 0.1mL
EUR 140

Rat Control IgG

MBS9144358-5x01mL 5x0.1mL
EUR 465

OOSA10361-1ML - Rat IgG2a NEGATIVE CONTROL Negative/Isotype Control

OOSA10361-1ML 1ml
EUR 169

SERION ELISA Avidity control Toxoplasma gondii Avidity Control IgG

MBS191654-INQUIRE INQUIRE Ask for price

Control for MSH2, 4 cases (1.5mm)HumanDigestive systemColonIHC control

MSH2081 1.5mm
EUR 50

Control for Olig2, 4 cases (1.5mm)HumanNervous systemBrainIHC control

OLIG2081 1.5mm
EUR 50

Goat IgG control

31R-AG001 1 mg
EUR 104
Description: Goat Immunoglobulins Goat IgG control

2'3'-cGAMP Control

tlrl-nagpap 1 mg
EUR 235.2

G3-YSD Control

tlrl-ydnac 200 µg
EUR 148.05

Rat IgG1 Control

mabg1-ctlrt 100 µg Ask for price

Goat IgG Control

GT15900 1 mg
EUR 199.2

Goat IgG control

MBS536227-1mg 1mg
EUR 255

Goat IgG control

MBS536227-5x1mg 5x1mg
EUR 1000

OOSA10379-250UG - Rat IgG2c NEGATIVE CONTROL Negative/Isotype Control

OOSA10379-250UG 0.25mg
EUR 319

Mouse IgG Control

10R-I169A 250 ug
EUR 104
Description: Mouse Immunoglobulins Mouse IgG Control

human IgM control

E4A11C04 50ug
EUR 255
Description: Available in various conjugation types.

human IgE control

E4A11C05 50ug
EUR 255
Description: Available in various conjugation types.

Mouse Control IgG

AC011 100 ul
EUR 143
Description: None

ISD Control Naked

tlrl-isdcn 200 µg
EUR 148.05

Rat IgG2a Control

mabg2a-ctlrt 100 µg
EUR 101.85

Rat IgG2a Control

mabg2a-ctlrt-05 5 x 100 µg
EUR 163.8

Rat IgG2b Control

mabg2b-ctlrt 100 µg Ask for price

Mouse IgM Control

ICIGMF-100 100 µg
EUR 239.4

Mouse IgM Control

ICIGMPU-100 100 µg
EUR 239.4

human IgM control

E4A11D04 50ug
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human IgE control

E4A11D05 50ug
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Mouse IgG Control

MBS532386-1mg 1mg
EUR 255

Mouse IgG Control

MBS532386-5x1mg 5x1mg
EUR 1000

Mouse Control IgG

AC040 100μL
EUR 56.68

Rabbit IgG Control

31R-AR001 500 ug
EUR 94
Description: Purified Normal Rabbit IgG

human IgG1 control

E4A11C03 50ug
EUR 255
Description: Available in various conjugation types.

Rabbit Control IgG

AC005 100 ul
EUR 143
Description: A synthetic peptide corresponding to Actin (plant specific).

ODN TTAGGG control

tlrl-ttagc-1 1 mg
EUR 546

Human IgA2 Control

maba2-ctrl 100 µg
EUR 101.85

Human IgA2 Control

maba2-ctrl-05 5 x 100 µg
EUR 163.8

Mouse Control IgG1

mabg1-ctrlm 100 µg
EUR 99.75

Mouse Control IgG1

mabg1-ctrlm-05 5 x 100 µg
EUR 163.8

Human IgG1 Control

HY-P9900 5mg
EUR 776.4

Mouse IgG1 Control

ICIGG1AC750-50 50 µg
EUR 855.6

Mouse IgG1 Control

ICIGG1PU-100 100 µg
EUR 239.4

Mouse IgG3 Control

ICIGG3CFB-100 100 µg
EUR 465.6

CD3 Control Slides

ICS2005-25 25 Slides
EUR 137.81

CD5 Control Slides

ICS2006-25 25 Slides
EUR 137.81

CD8 Control Slides

ICS2007-25 25 Slides
EUR 137.81

CEA Control Slides

ICS2012-25 25 Slides
EUR 137.81

EMA Control Slides

ICS2018-25 25 Slides
EUR 137.81

LCA Control Slides

ICS2029-25 25 Slides
EUR 137.81

NSE Control Slides

ICS2031-25 25 Slides
EUR 137.81

PSA Control Slides

ICS2033-25 25 Slides
EUR 137.81

PAS Control Slides

HCS1016-25 25 Slides
EUR 137.81

Rabbit IgG Control

GWB-DLRABG 0.5 mg Ask for price

Human IgG1 Control

TP1357-10mg 10mg Ask for price
Description: Human IgG1 Control

Human IgG1 Control

TP1357-1g 1g Ask for price
Description: Human IgG1 Control

Human IgG1 Control

TP1357-1mg 1mg Ask for price
Description: Human IgG1 Control

Human IgG1 Control

TP1357-50mg 50mg Ask for price
Description: Human IgG1 Control

Human IgG1 Control

TP1357-5mg 5mg Ask for price
Description: Human IgG1 Control

human IgG1 control

E4A11D03 50ug
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

IgM, Monkey, Control

MBS654675-005mg 0.05mg
EUR 575

IgM, Monkey, Control

MBS654675-5x005mg 5x0.05mg
EUR 2440

Human IgG1 Control

MBS5763617-1mg 1mg
EUR 210

Human IgG1 Control

MBS5763617-5mg 5mg
EUR 445

Human IgG1 Control

MBS5763617-5x5mg 5x5mg
EUR 1850

Rabbit IgG Control

MBS539655-05mg 0.5mg
EUR 425

Rabbit IgG Control

MBS539655-5x05mg 5x0.5mg
EUR 1770

pBIND-Id Control

PVTY00606 2ug
EUR 280

ISD Control (sodium)

HY-160226 Get quote Ask for price
Description: ISD (interferon stimulatory DNA) Control sodium is a non-immunostimulatory single-stranded oligonucleotide with the same sequence as ISD, its double-stranded counterpart[1].

EUROSTAR 200 control Overhead Stirrer with wireless control unit - EACH

STI2876 EACH
EUR 6112.8

EUROSTAR 100 control Overhead Stirrer with wireless control unit - EACH

STI2878 EACH
EUR 4104

RNA Extraction Control 670

BIO-38040 100 Reactions Ask for price

RNA Extraction Control 670

BIO-38041 500 Reactions Ask for price

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