fondationvocation
Algemeen / Anders / Assay / Isotype

van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel

- June 11, 2021April 30, 2021 - annelies

Kwantitatieve bepaling van sporenmetaal in celkweekmedia met behulp van LS-APGD-MS en ICP-OES met differentiatie van vrije / gebonden soorten na scheidingen van polymeervezels

Liquid sampling-atmospheric pressure glow discharge-mass spectrometry (LS-APGD-MS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES) were employed for the quantification of trace metals in cell culture media and their capabilities compared. The LS-APGD is interfaced here to a compact mass spectrometer (Advion CMS) towards the development of an at-bioreactor process monitoring strategy. Both techniques have been previously employed for the quantification of trace metals in samples of various complexities, making them a natural choice for this application.

They have also demonstrated comparable analytical figures of merit including limits of detection (LOD), matrix tolerance, etc. While cell culture media is a complex sample, the ICP-OES technique was unaffected by the matrix. However, the LS-APGD-MS suffered from increases in spectral background. Despite this, both techniques achieved appropriate LODs for all metals analyzed in this work (Cu, Fe, Zn, Co, Mn, Ni; LOD < 100 ng mL-1), except for Mn and Ni via LS-APGD-MS. To overcome the increased background seen on the LS-APGD-MS, a capillary channeled polymer (C-CP) polypropylene (PPY) fiber stationary phase was employed as an on-line separation for the removal of organic components prior to sample introduction into the plasma.
It was further determined that Ni was retained on the column, preventing the detection of this element via LS-APGD-MS, and insights into metal speciation were discussed. Following implementation of this on-line separation strategy, the agreement between the techniques was acceptable for all analytes, and was excellent for Cu, Fe, and Zn.

Co-gereguleerde pilus van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel van Caco-2 en perifere bloedmononucleaire cellen (PBMC), wat leidt tot verhoogde kolonisatie

The aim of this study was to assess the modulatory effect of TcpA in the expression of CEACAM1 adhesin molecule and IL-1, IL-8 and TNFα pro-inflammatiory cytokines in the co-culture model of Caco-2/PBMC that can mimic the intestinal milieu. The tcpA gene from V.cholerae ATCC14035 was cloned in pET-28a and transformed into E.coli Bl-21. The recombinant TcpA-His6 protein was expressed and purified using the Ni-column chromatography.
The sequencing of transformed plasmid and Western blotting of purified protein confirmed the identity of rTcp. Cytotoxicity of different concentrations of recombinant protein for human colon carcinoma cell line (Caco-2 cell) was assessed by MTT assay and showed viability of 92%, 82% and 70 %, for10 µg/mL of TcpA after 24, 48 and 72 h, respectively. Co-cultures of Caco-2 and peripheral blood mononuclear cells (PBMCs) were used to mimic the intestinal milieu and treated with different concentrations of rTcpA (1, 5, 10 and 50 µg/mL).
Our data showed about 2.04, 3.37, 3.68 and 42.7-folds increase in CEACAM1 gene expression, respectively, compared with non-treated Caco-2/PBMC co-culture. Moreover, the expression of IL-1, IL-8 and TNFα genes was significantly increased up to 15.75, 7.04 and 80.95 folds, respectively. In conclusion, V.cholerae TcpA induces statistically significant dose-dependent stimulatory effect on TNFα, IL-1 and IL-8 pro-inflammatory cytokines expression. Among all, TNFα was much more affected which consequently, elevated CEACAM1 expression level in IECs. This suggests TcpA protein as critical effector as an inducer of increased adhesion potential of V. cholera as well as inflammatory responses of host intestinal tissue. This article is protected by copyright. All rights reserved.
fondationvocation
fondationvocation

pAAV-RC9 vector
PVT12073 2 ug
EUR 524

pAAV-DJ vector
PVT12151 2 ug
EUR 438

pAAV-DJ Vector
VPK-420-DJ 10 µg
EUR 647
Description: The pAAV-DJ vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ packaging.

pAAV-RC1 Vector
VPK-421 10 µg
EUR 647
Description: The pAAV-RC1 vector contains the rep and cap genes required to generated recombinant AAV of serotype 1. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-1 packaging.

pAAV-RC3 Vector
VPK-423 10 µg
EUR 647
Description: The pAAV-RC3 vector contains the rep and cap genes required to generated recombinant AAV of serotype 3. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-3 packaging.

pAAV-RC4 Vector
VPK-424 10 µg
EUR 647
Description: The pAAV-RC4 vector contains the rep and cap genes required to generated recombinant AAV of serotype 4. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-4 packaging.

pAAV-RC5 Vector
VPK-425 10 µg
EUR 647
Description: The pAAV-RC5 vector contains the rep and cap genes required to generated recombinant AAV of serotype 5. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-5 packaging.

pAAV-RC6 Vector
VPK-426 10 µg
EUR 647
Description: The pAAV-RC6 vector contains the rep and cap genes required to generated recombinant AAV of serotype 6. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-6 packaging.

pAAV-RC6
PVT14647 2 ug
EUR 703

pAAV- RC
PVT2103 2 ug
EUR 241

pAAV-GFP Control Vector
AAV-400 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-Cre Control Vector
AAV-401 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-LacZ Control Vector
AAV-402 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-MCS Expression Vector
VPK-410 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-DJ/8 Vector
VPK-420-DJ-8 10 µg
EUR 647
Description: The pAAV-DJ/8 vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ/8. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ/8 packaging.

pAAV- IRES- ZsGreen1
PVT11044 2 ug
EUR 301

pAAV- ZsGreen1- shRNA
PVT11045 2 ug
EUR 370

pAAV-fNPY-GFP
PVT14636 2 ug
EUR 599

pAAV-RSV-SpCas9
PVT17629 2 ug
EUR 300

pAAV-CAG-GFP
PVT17666 2 ug
EUR 341

pAAV- MCS Plasmid
PVT2102 2 ug
EUR 241

pAAV-MCS Promoterless Expression Vector
VPK-411 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Puro Expression Vector
VPK-415 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Neo Expression Vector
VPK-416 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector
VPK-417 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector
VPK-418 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Bsd Expression Vector
VPK-419 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-EF1a-DIO-mCherry
PVT17841 2 ug
EUR 300

pAAV- IRES- hrGFP Plasmid
PVT2104 2 ug
EUR 266

Human Topoisomerase I
TG2005H-RC2 500 units
EUR 448

pAAV- CMV- mCherry- U6- sgRNA
PVT11046 2 ug
EUR 301

pAAV-hSyn-hChR2(H134R)-mCherry
PVT19026 2 ug
EUR 258

pAAV-hSyn-eNpHR 3.0-EYFP
PVT19063 2 ug
EUR 258

pAAV-EF1a-DIO-hM3D(Gq)-mCherry
PVT17847 2 ug
EUR 300

pAAV-MCS-Ppargc1a-m-FLAG-HA
PVT18341 2 ug
EUR 300

pVL1392 Vector
ABP-BVP-10001 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids

pVL1393 Vector
ABP-BVP-10002 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids

pORB Vector
ABP-BVP-10003 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids

pAcSec1 Vector
ABP-BVP-10004 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids

pAcIRES Vector
ABP-BVP-10005 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids

pEE14.4 vector
PVT11901 2 ug
EUR 1036

pENTR223.1 vector
PVT11990 2 ug
EUR 352

pUB_smFLAG_KDM5B_MS2 vector
PVT11991 2 ug
EUR 352

ER2738 vector
PVT11993 2 ug
EUR 352

pFLPo vector
PVT12063 2 ug
EUR 352

pREDKI vector
PVT12064 2 ug
EUR 352

pREDCas9 vector
PVT12065 2 ug
EUR 352

PWUR790 vector
PVT12066 2 ug
EUR 352

xCas9 vector
PVT12068 2 ug
EUR 352

sgRNA vector
PVT12071 2 ug
EUR 352

PY094 vector
PVT12150 2 ug
EUR 352

PY094 vector
PVT12150-1 2 ug
EUR 352

pRGEB31 vector
PVT12152 2 ug
EUR 352

pSET152 vector
PVT3395 2 ug
EUR 376

pYLEX1 - Expression Vector
FYY203-5MG 5mg Ask for price

pYLSC1- Secretion Vector
FYY204-5MG 5mg Ask for price

pMRNAxp mRNAExpress Vector
MR000PA-1 10 ug
EUR 900
  • Category: Stem Cell Products

pMXs Retroviral Vector
RTV-010 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMYs Retroviral Vector
RTV-020 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMZs Retroviral Vector
RTV-030 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pENTR223-ATP5PO vector
PVT11685 2 ug
EUR 304

pENTR223- LC25A10 vector
PVT11686 2 ug
EUR 304

pENTR223-RSU1 vector
PVT11687 2 ug
EUR 304

pENTR223-CCDC24 vector
PVT11688 2 ug
EUR 304

pENTR223-BAT2L vector
PVT11689 2 ug
EUR 304

pENTR223-RSPH14 vector
PVT11690 2 ug
EUR 304

pENTR223-ABHD5 vector
PVT11691 2 ug
EUR 304

pENTR223-NONO vector
PVT11692 2 ug
EUR 304

pENTR223-MYOZ1 vector
PVT11693 2 ug
EUR 304

pENTR223-STX5 vector
PVT11694 2 ug
EUR 304

pENTR223-GK5 vector
PVT11695 2 ug
EUR 304

pENTR223-PIGC vector
PVT11696 2 ug
EUR 304

pENTR223-FCGR3A vector
PVT11697 2 ug
EUR 304

pENTR223-ANXA8L1 vector
PVT11698 2 ug
EUR 304

pENTR223-CDK5 vector
PVT11699 2 ug
EUR 304

pENTR223-ERP27 vector
PVT11700 2 ug
EUR 304

pENTR223-ZDHHC16 vector
PVT11701 2 ug
EUR 304

pENTR223-DFNA5 vector
PVT11702 2 ug
EUR 304

pENTR223-MPPED2 vector
PVT11703 2 ug
EUR 304

pENTR223-NIFK vector
PVT11704 2 ug
EUR 304

pENTR223-KCTD17 vector
PVT11705 2 ug
EUR 304

pENTR223-CEP170P1 vector
PVT11706 2 ug
EUR 304

pENTR223-SCML4 vector
PVT11707 2 ug
EUR 304

pENTR223-CLEC18C vector
PVT11708 2 ug
EUR 304

pENTR223-HSD17B11 vector
PVT11709 2 ug
EUR 304

pENTR223-UBXN1 vector
PVT11710 2 ug
EUR 304

pENTR223-MRPL4 vector
PVT11714 2 ug
EUR 304

pENTR223-DECR2 vector
PVT11715 2 ug
EUR 304

pENTR223-GNB3 vector
PVT11716 2 ug
EUR 304

pENTR223-FAM118B vector
PVT11717 2 ug
EUR 304

pENTR223-EIF2S2 vector
PVT11718 2 ug
EUR 304

pENTR223-HDAC11 vector
PVT11720 2 ug
EUR 304

pENTR223-RDH11 vector
PVT11721 2 ug
EUR 304

pENTR223-GNB1L vector
PVT11722 2 ug
EUR 304

pENTR223-BCL2L14 vector
PVT11723 2 ug
EUR 304

pENTR223-RPLP0 vector
PVT11724 2 ug
EUR 304

pENTR223-RALYL vector
PVT11725 2 ug
EUR 304

pENTR223-GPM6A vector
PVT11726 2 ug
EUR 304

pENTR223-BLVRA vector
PVT11727 2 ug
EUR 304

Een nieuwe isolatie- en kweekmethode voor granulosacellen

Granulosa cells are the cell population who have an increasing importance in the female genital system and reproduction. Thus, nowadays in vitro studies to address these cells are also gaining importance and attracts researcher’s attention. The aim of our study is to develop a more feasible, low-cost granulosa cell isolation and culture method compared to methods defined so far.
Granulosa cells were isolated from follicular fluids obtained from both healthy women donors (n = 19) and polycystic ovary syndrome (n = 15) applied to in vitro fertilization treatment process. Granulosa cells were isolated by using Lymphosep® separation fluid that was not used for this purpose before. The isolated cells were cultured in suitable culture dishes with a mixture of BIO-AMFTM-1 and DMEM/F12 in the first seeding and only complete DMEM/F12 in the following feeds.
  • The complete medium contains only 5% fetal calf serum, 4% L-glutamine and 1% penicillin-streptomycin-amphotericin. The new methods we have developed in granulosa cell isolation and in vitro culture have been successful.
  • Reduction in supplement types and amount; improved the proliferation rate of the granulosa cells in culture. Our new methods of isolation and cell culture for granulosa cells from healthy women, have been also successful in samples of polycystic ovarian patients.
  • With these developed methods, granulosa cells, which belong to humans and have an important role in the ovary, could be isolated and subsequently be maintained to reproduce (proliferate) more easily and cheaper.

Over de oorsprong van SARS-CoV-2: hebben celkweekexperimenten geleid tot verhoogde virulentie van het voorlopervirus voor mensen?

We are currently in a rapidly expanding pandemic of the SARS-CoV-2 virus, which originated in the city of Wuhan in central China. The disease COVID-19 is now spread worldwide and has tremendous socio-economic consequences. The origin of the virus can be reconstructed through epidemiological studies and, even more so, from genome comparisons. How the evolution of the virus and the transition to humans might have happened is the subject of much speculation.
It is considered certain that the virus is of animal origin and very likely passed from bats to humans in a zoonotic event. An intermediate host was postulated, but many SARS-like bat viruses have the ability to infect human cells directly, which has been shown experimentally by scientists in the Wuhan Institute of Virology using collected specimens containing virus material from horseshoe bats.
The propagation of SARS-like bat viruses in cell culture allowed experiments aimed at increasing the infectivity of the virus and adaptation to human cells. This article summarizes the unique properties of SARS-CoV-2 and focusses on a specific sequence encoding the spike protein.
Possible scenarios of virus evolution are discussed, with particular emphasis on the hypothesis that the virus could have emerged unintentionally through routine culture or gain-of-function experiments in a laboratory, where it was optimally adapted to human cells and caused cryptic infections among workers who finally spread the virus causing the pandemic.

 

BSA Control for Age-BSA
2221-BSA
EUR 175

Cotinine-BSA Conjugate
COTN15-BSA 0.5 mg
EUR 286

Positive control tissue section for each antibody; Based on availability INQUIRE
Control-Slides Set of 5
EUR 176

BSA Control for AGE-BSA
35R-AA007 10 mg
EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

CEL-BSA Control
STA-302 100 µg
EUR 322
Description:
  • N-epsilon-(carboxyethyl) lysine (CEL) modified BSA may be used as a positive control. 100 µg at 1 mg/mL in PBS.
  • Provided at 1 mg/mL in 1X PBS

MG-BSA Control
STA-306 100 µg
EUR 322
Description:
  • Methylglyoxal-BSA (MG-BSA) may be used as a positive control. 100 µg at 1 mg/mL in PBS.
  • Provided at 1 mg/mL

CML-BSA Control
STA-314 100 µg
EUR 322
Description:
  • CML-BSA may be used as a positive control. 100 µg.
  • Provided at 1 mg/mL in 1X PBS

MDA-BSA Control
STA-333 100 µg
EUR 322
Description:
  • MDA-BSA (Malondialdehyde-BSA) may be used as a positive control. 100 µg.
  • Provided at 1 mg/mL in 1X PBS

Bovine Serum Albumin Biotinylated (Biotin-BSA)
BTN-BSA 5 mg
EUR 286

Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate
IBT15-BSA 100 ug
EUR 529

Phosphoserine-BSA control protein
PSER15-N 100 ug
EUR 164

Dinitrophenyl (DNP)-BSA protein Conjugate
AV-9330-BSA 10 mg
EUR 286

mPEG-BSA (Molecular Weight: 5,000-linear)
PEG-BSA-05K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 10,000-linear)
PEG-BSA-10K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 20,000-linear)
PEG-BSA-20K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 40,000-linear)
PEG-BSA-40K 100 ug
EUR 286

HNE antibody
20R-HG007 100 ug
EUR 300
Description: Goat polyclonal HNE antibody

4-Hydroxynonenal (HNE)-BSA protein conjugate for ELISA (in PBS)
HNE15-R 100 ug
EUR 347

(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA
DRAA31-BSA 0.5 mg
EUR 529

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA
PPPP321-BSA 0.5 mg
EUR 529

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA
NVDP41-BSA 0.5 mg
EUR 529

(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA
NANP51-BSA 0.5 mg
EUR 529

Protein Tyrosine Nitration Control (Nitrotyrosine-BSA)
STA-304 10 ?g
EUR 241
Description:
  • Protein Tyrosine Nitration Control (Nitrotyrosine-BSA) may be used as a positive control. 10 µg.
  • Provided at 0.1 mg/mL

Oxidized Protein Immunoblot Control (Carbonyl-BSA)
STA-309 10 ?g
EUR 241
Description:
  • Oxidized Protein Immunoblot Control (Carbonyl-BSA) may be used as a positive control. 10 µg.
  • Provided at 50 µg/mL

Bovine Serum Albumin (BSA) ELISA Kit
DLR-BSA-Ge-48T 48T
EUR 387
  • Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) ELISA Kit
DLR-BSA-Ge-96T 96T
EUR 494
  • Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)
SPR-211A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies 4-Hydroxynonenal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)
SPR-211B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies 4-Hydroxynonenal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)
SPR-211C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies 4-Hydroxynonenal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column
800-302-BSA 1 Kit
EUR 347

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column
800-310-BSA 1 Kit
EUR 895

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column
800-325-BSA 1 Kit
EUR 1450

General Bovine Serum Albumin (BSA) ELISA Kit
RDR-BSA-Ge-48Tests 48 Tests
EUR 551

General Bovine Serum Albumin (BSA) ELISA Kit
RDR-BSA-Ge-96Tests 96 Tests
EUR 766

General Bovine Serum Albumin (BSA) ELISA Kit
RD-BSA-Ge-48Tests 48 Tests
EUR 527

General Bovine Serum Albumin (BSA) ELISA Kit
RD-BSA-Ge-96Tests 96 Tests
EUR 732

Monoclonal Anti-4 HNE (4-HNE; 4-hydroxynonenal) IgG
HNE13-M 100 ul
EUR 482

4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)
HNE11-C 100 ul
EUR 286

4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)
HNE12-C 100 ul
EUR 286

Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant
AV-1010-BSA 1 ml
EUR 286

Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit
BCA-BSA-1 1 pk
EUR 164

Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit
BCA-BSA-750 1 ml
EUR 225

BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit
BSA-BGG-1 1 pk
EUR 286

Polyclonal HNE / Neutrophil Elastase Antibody
APS00008G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Sheep that recognizes and binds to Human HNE / Neutrophil Elastase . This antibody is tested and proven to work in the following applications:

Human 4-HNE ELISA Kit
EHH0210 96Tests
EUR 521

Goat 4-HNE ELISA Kit
EGTH0210 96Tests
EUR 521

Bovine 4-HNE ELISA Kit
EBH0210 96Tests
EUR 521

Canine 4-HNE ELISA Kit
ECH0210 96Tests
EUR 521

Chicken 4-HNE ELISA Kit
ECKH0210 96Tests
EUR 521

4-HNE ELISA KIT|Human
EF006163 96 Tests
EUR 689

4-Hydroxynonenal (4-HNE) Antibody
abx411903-01ml 0.1 ml
EUR 509
  • Shipped within 1 week.

4 hydroxynonenal (HNE), >98% pure
HNE51-1 1 mg
EUR 202

4 hydroxynonenal (HNE), >98% pure
HNE51-5 5 mg
EUR 651

Rat 4-HNE ELISA Kit
ERH0210 96Tests
EUR 521

Sheep 4-HNE ELISA Kit
ESH0210 96Tests
EUR 521

Rabbit 4-HNE ELISA Kit
ERTH0210 96Tests
EUR 521

Mouse 4-HNE ELISA Kit
EMH0210 96Tests
EUR 521

Monkey 4-HNE ELISA Kit
EMKH0210 96Tests
EUR 521

Porcine 4-HNE ELISA Kit
EPH0210 96Tests
EUR 521

anti-4-HNE (HNEJ-2)
LF-MA90010 20 ug
EUR 360
Description: Mouse monoclonal to 4-HNE

anti-4-HNE (HNEJ-2)
LF-MA90011 100 ug
EUR 891
Description: Mouse monoclonal to 4-HNE

Malondialdehyde (MDA)-BSA Conjugate positive control for Western/ELISA
MDA35-N-100 100 ug
EUR 286

Pre-diluted BSA (bovine serum albumin) standards (250, 125, 62.5, 31.25, and 0.0 ug/ml) for BCA Optima Protein Assay kit
BCA-BSA-2 1 pk
EUR 164

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column
800-200-BSA 1 kit
EUR 286

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column
800-205-BSA 1 kit
EUR 773

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column
800-225-BSA 1 kit
EUR 2425

Rat 4-Hydroxynonenal(HNE)ELISA Kit
CSB-EQ027232RA-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Rat 4-Hydroxynonenal (HNE) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat 4-Hydroxynonenal(HNE)ELISA Kit
1-CSB-EQ027232RA
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Rat 4-Hydroxynonenal(HNE) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

4-Hydroxynonenal (4-HNE) ELISA Kit
abx257639-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.

Guinea Pig 4-HNE ELISA Kit
EGH0210 96Tests
EUR 521

4-Hydroxynonenal (4-HNE) ELISA Kit
E4645-100
EUR 865

Mouse 4-Hydroxynonenal (HNE)ELISA Kit
CSB-E13412m-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse 4-Hydroxynonenal (HNE) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse 4-Hydroxynonenal (HNE)ELISA Kit
1-CSB-E13412m
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse 4-Hydroxynonenal (HNE) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human 4-Hydroxynonenal (HNE)ELISA Kit
CSB-E16214h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Human 4-Hydroxynonenal (HNE) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human 4-Hydroxynonenal (HNE)ELISA Kit
1-CSB-E16214h
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Human 4-Hydroxynonenal (HNE) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit Anti-4 hydroxynonenal (HNE) antiserum
HNE11-S 100 ul
EUR 457

OxiSelect HNE Adduct Competitive ELISA Kit
STA-838 96 assays
EUR 798
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

OxiSelect HNE Adduct Competitive ELISA Kit
STA-838-5 5 x 96 assays
EUR 3240
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

ELISA kit for 4-HNE (4-Hydroxynonenal)
E-EL-0128 1 plate of 96 wells
EUR 377
  • Gentaur's 4-HNE ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid pha
  • Show more
Description: A competitive ELISA kit for quantitative measurement of 4-HNE (4-Hydroxynonenal) in samples from Serum, Plasma, Cell supernatant

Mouse 4-Hydroxynonenal (4-HNE) ELISA Kit
abx257660-96tests 96 tests
EUR 754
  • Shipped within 5-12 working days.

Human 4-Hydroxynonenal (4-HNE) ELISA Kit
abx251411-96tests 96 tests
EUR 754
  • Shipped within 5-12 working days.

Human 4-HNE(4-Hydroxynonenal) ELISA Kit
EH2080 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
  • Alias: 4-HNE(4-Hydroxynonenal)/HNE/4 HNE
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 pg/ml

Goat Anti-4 hydroxynonenal (HNE) antiserum #2
HNE12-S 100 ul
EUR 457

Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit
EM1583 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Rat 4-HNE(4-Hydroxynonenal) ELISA Kit
ER1587 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 18.75 pg/ml

Goat Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody
STA-034 100 µg
EUR 664
Description: Goat Anti-4-hydroxynonenal Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

Rabbit Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody
STA-035 100 µg
EUR 664
Description: Rabbit Anti-4-hydroxynonenal Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

BSA
31C-CH0114 50 mg
EUR 133
Description: Bovine Serum Albumin for use in the research laboratory

Testosterone 3 BSA Protein (BSA)
abx060856-1mg 1 mg
EUR 578
  • Shipped within 5-10 working days.

Ibrutinib drug-Human Serum Albumin Conjugate
IBT16-BSA 100 ug
EUR 529

Mouse (mrl/lpr) serum containing autoantibodies to HNE
HNE31-M 100 ul
EUR 482

OxiSelect HNE Adduct Competitive ELISA Kit, Trial Size
STA-838-T 32 assays
EUR 421
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

Control siRNA Vector (pGB-control)
9500C-20
EUR 338

Histamine [G-BSA] (G-G-BSA)
DAG3327 1mg
EUR 1234

Cyclic citrullinated peptide-BSA（CCP-BSA）
E64I00802 100ug
EUR 343

AGE-BSA
2221-10
EUR 370

AGE-BSA
2221-50 Ask for price

BSA antibody
20R-1871 100 ug
EUR 673
Description: Rabbit polyclonal BSA antibody

BSA antibody
20R-AR042 20 mg
EUR 327
Description: Rabbit polyclonal BSA antibody

Leave a Reply

Your email address will not be published. Required fields are marked *