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Algemeen / Anders / Assay / Isotype

van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel

- juni 11, 2021april 30, 2021 - annelies

Kwantitatieve bepaling van sporenmetaal in celkweekmedia met behulp van LS-APGD-MS en ICP-OES met differentiatie van vrije / gebonden soorten na scheidingen van polymeervezels

Liquid sampling-atmospheric pressure glow discharge-mass spectrometry (LS-APGD-MS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES) were employed for the quantification of trace metals in cell culture media and their capabilities compared. The LS-APGD is interfaced here to a compact mass spectrometer (Advion CMS) towards the development of an at-bioreactor process monitoring strategy. Both techniques have been previously employed for the quantification of trace metals in samples of various complexities, making them a natural choice for this application.

They have also demonstrated comparable analytical figures of merit including limits of detection (LOD), matrix tolerance, etc. While cell culture media is a complex sample, the ICP-OES technique was unaffected by the matrix. However, the LS-APGD-MS suffered from increases in spectral background. Despite this, both techniques achieved appropriate LODs for all metals analyzed in this work (Cu, Fe, Zn, Co, Mn, Ni; LOD < 100 ng mL-1), except for Mn and Ni via LS-APGD-MS. To overcome the increased background seen on the LS-APGD-MS, a capillary channeled polymer (C-CP) polypropylene (PPY) fiber stationary phase was employed as an on-line separation for the removal of organic components prior to sample introduction into the plasma.
It was further determined that Ni was retained on the column, preventing the detection of this element via LS-APGD-MS, and insights into metal speciation were discussed. Following implementation of this on-line separation strategy, the agreement between the techniques was acceptable for all analytes, and was excellent for Cu, Fe, and Zn.

Co-gereguleerde pilus van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel van Caco-2 en perifere bloedmononucleaire cellen (PBMC), wat leidt tot verhoogde kolonisatie

The aim of this study was to assess the modulatory effect of TcpA in the expression of CEACAM1 adhesin molecule and IL-1, IL-8 and TNFα pro-inflammatiory cytokines in the co-culture model of Caco-2/PBMC that can mimic the intestinal milieu. The tcpA gene from V.cholerae ATCC14035 was cloned in pET-28a and transformed into E.coli Bl-21. The recombinant TcpA-His6 protein was expressed and purified using the Ni-column chromatography.
The sequencing of transformed plasmid and Western blotting of purified protein confirmed the identity of rTcp. Cytotoxicity of different concentrations of recombinant protein for human colon carcinoma cell line (Caco-2 cell) was assessed by MTT assay and showed viability of 92%, 82% and 70 %, for10 µg/mL of TcpA after 24, 48 and 72 h, respectively. Co-cultures of Caco-2 and peripheral blood mononuclear cells (PBMCs) were used to mimic the intestinal milieu and treated with different concentrations of rTcpA (1, 5, 10 and 50 µg/mL).
Our data showed about 2.04, 3.37, 3.68 and 42.7-folds increase in CEACAM1 gene expression, respectively, compared with non-treated Caco-2/PBMC co-culture. Moreover, the expression of IL-1, IL-8 and TNFα genes was significantly increased up to 15.75, 7.04 and 80.95 folds, respectively. In conclusion, V.cholerae TcpA induces statistically significant dose-dependent stimulatory effect on TNFα, IL-1 and IL-8 pro-inflammatory cytokines expression. Among all, TNFα was much more affected which consequently, elevated CEACAM1 expression level in IECs. This suggests TcpA protein as critical effector as an inducer of increased adhesion potential of V. cholera as well as inflammatory responses of host intestinal tissue. This article is protected by copyright. All rights reserved.
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Een nieuwe isolatie- en kweekmethode voor granulosacellen

Granulosa cells are the cell population who have an increasing importance in the female genital system and reproduction. Thus, nowadays in vitro studies to address these cells are also gaining importance and attracts researcher’s attention. The aim of our study is to develop a more feasible, low-cost granulosa cell isolation and culture method compared to methods defined so far.
Granulosa cells were isolated from follicular fluids obtained from both healthy women donors (n = 19) and polycystic ovary syndrome (n = 15) applied to in vitro fertilization treatment process. Granulosa cells were isolated by using Lymphosep® separation fluid that was not used for this purpose before. The isolated cells were cultured in suitable culture dishes with a mixture of BIO-AMFTM-1 and DMEM/F12 in the first seeding and only complete DMEM/F12 in the following feeds.
  • The complete medium contains only 5% fetal calf serum, 4% L-glutamine and 1% penicillin-streptomycin-amphotericin. The new methods we have developed in granulosa cell isolation and in vitro culture have been successful.
  • Reduction in supplement types and amount; improved the proliferation rate of the granulosa cells in culture. Our new methods of isolation and cell culture for granulosa cells from healthy women, have been also successful in samples of polycystic ovarian patients.
  • With these developed methods, granulosa cells, which belong to humans and have an important role in the ovary, could be isolated and subsequently be maintained to reproduce (proliferate) more easily and cheaper.

Over de oorsprong van SARS-CoV-2: hebben celkweekexperimenten geleid tot verhoogde virulentie van het voorlopervirus voor mensen?

We are currently in a rapidly expanding pandemic of the SARS-CoV-2 virus, which originated in the city of Wuhan in central China. The disease COVID-19 is now spread worldwide and has tremendous socio-economic consequences. The origin of the virus can be reconstructed through epidemiological studies and, even more so, from genome comparisons. How the evolution of the virus and the transition to humans might have happened is the subject of much speculation.
It is considered certain that the virus is of animal origin and very likely passed from bats to humans in a zoonotic event. An intermediate host was postulated, but many SARS-like bat viruses have the ability to infect human cells directly, which has been shown experimentally by scientists in the Wuhan Institute of Virology using collected specimens containing virus material from horseshoe bats.
The propagation of SARS-like bat viruses in cell culture allowed experiments aimed at increasing the infectivity of the virus and adaptation to human cells. This article summarizes the unique properties of SARS-CoV-2 and focusses on a specific sequence encoding the spike protein.
Possible scenarios of virus evolution are discussed, with particular emphasis on the hypothesis that the virus could have emerged unintentionally through routine culture or gain-of-function experiments in a laboratory, where it was optimally adapted to human cells and caused cryptic infections among workers who finally spread the virus causing the pandemic.

 

BSA Control for AGE-BSA
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710-113-PC 1
EUR 196.8

Rabbit Anti-Bovine Serum Albumin (BSA) IgG negative control (0 U/ml, 1 ml)
710-113-NC 1 ml
EUR 123.6

pMD18- Control
PVT10563 2 ug
EUR 319.2

pMD19- Control
PVT10564 2 ug
EUR 319.2

Rat neurofascin (Nfasc)-control Control/blocking peptide
AB-23249-CP 100ug
EUR 196.8

Quality Control
abx098966-1vial 1 vial
EUR 360

Rat IgG-PE conjugate (isotype control) (Isotype control)
20005-PE 25 tests
EUR 242.4

Rat IgG-HRP conjugate (isotype control) (Isotype control)
20005-HP 100 ug
EUR 196.8

Rat IgG-FITC conjugate (isotype control) (Isotype control)
20005-F 100 ug
EUR 196.8

PIPET CONTROLLER CONTROL BOARD
357484 1/pk
EUR 104.4
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories

Rat IgG-Biotin conjugate (isotype control) (Isotype control)
20005-B 100 ug
EUR 196.8

Goat IgG (-ve control for flow cytometry) (isotype control)
20011-100 100 test
EUR 123.6

Control siRNA Vector (pGB-control)
9500C-20 each
EUR 405.6

Rat Control IgG
AC034 100 ul
EUR 159.6

Goat IgG control
31R-AG001 1 mg
EUR 152.4
Description: Goat Immunoglobulins Goat IgG control

Goat IgG Control
GT15900 1 mg
EUR 199.2

Mouse IgG Control
10R-I169A 250 ug
EUR 152.4
Description: Mouse Immunoglobulins Mouse IgG Control

Mouse Control IgG
AC011 100 ul
EUR 159.6

Mouse IgM Control
ICIGMF-100 100 µg
EUR 239.4

Mouse IgM Control
ICIGMPU-100 100 µg
EUR 239.4

EUROSTAR 200 control Overhead Stirrer with wireless control unit
STI2876 EACH
EUR 5433.6

EUROSTAR 100 control Overhead Stirrer with wireless control unit
STI2878 EACH
EUR 3648

Rabbit Control IgG
AC005 100 ul
EUR 159.6

Rabbit IgG Control
31R-AR001 500 ug
EUR 159.6
Description: Purified Normal Rabbit IgG

Human IgG1 Control
HY-P9900 5mg
EUR 776.4

Mouse IgG1 Control
ICIGG1A-50 50 µg
EUR 387.6

Mouse IgG1 Control
ICIGG1AC750-50 50 µg
EUR 855.6

Mouse IgG1 Control
ICIGG1CFB-100 100 µg
EUR 387.6

Mouse IgG1 Control
ICIGG1F-100 100 µg
EUR 239.4

Mouse IgG1 Control
ICIGG1PE-50 50 µg
EUR 387.6

Mouse IgG1 Control
ICIGG1PP-100 100 µg
EUR 387.6

Mouse IgG1 Control
ICIGG1PP5.5-100 100 µg
EUR 387.6

Mouse IgG1 Control
ICIGG1PU-100 100 µg
EUR 239.4

Mouse IgG3 Control
ICIGG3A-50 100 µg
EUR 777.6

Mouse IgG3 Control
ICIGG3CFB-100 100 µg
EUR 465.6

Mouse IgG3 Control
ICIGG3F-100 100 µg
EUR 387.6

Mouse IgG3 Control
ICIGG3PE-50 50 µg
EUR 621.6

Mouse IgG3 Control
ICIGG3PU-100 100 µg
EUR 387.6

VACUUM CONTROL KIT
ZAFSVCKIT EACH
EUR 381.24

EUROSTAR 60 control Overhead Stirrer with wireless control unit
STI2886 EACH
EUR 2894.4

Vacuum Controller w Control Unit
PUM3016 EACH
EUR 2559.6

RNA Extraction Control 670
BIO-38040 100 Reactions Ask for price

RNA Extraction Control 670
BIO-38041 500 Reactions Ask for price

RNA Extraction Control 560
BIO-38044 100 Reactions Ask for price

RNA Extraction Control 560
BIO-38045 500 Reactions Ask for price

RNA Extraction Control 610
BIO-38048 100 Reactions Ask for price

RNA Extraction Control 610
BIO-38049 500 Reactions Ask for price

Mouse IgG2a Control
ICIGG2AA-50 50 µg
EUR 387.6

Mouse IgG2a Control
ICIGG2AB-100 100 µg
EUR 239.4

Mouse IgG2a Control
ICIGG2ACFB-100 100 µg
EUR 387.6

Mouse IgG2a Control
ICIGG2AF-100 100 µg
EUR 239.4

Mouse IgG2a Control
ICIGG2APE-50 50 µg
EUR 387.6

Mouse IgG2a Control
ICIGG2APP-100 100 µg
EUR 387.6

Mouse IgG2a Control
ICIGG2APP5.5-100 100 µg
EUR 387.6

Mouse IgG2a Control
ICIGG2APU-100 100 µg
EUR 239.4

Mouse IgG2b Control
ICIGG2BA-50 50 µg
EUR 387.6

Mouse IgG2b Control
ICIGG2BCFB-100 100 µg
EUR 387.6

Mouse IgG2b Control
ICIGG2BF-100 100 µg
EUR 239.4

Mouse IgG2b Control
ICIGG2BPE-50 50 µg
EUR 387.6

Mouse IgG2b Control
ICIGG2BPP-100 100 µg
EUR 387.6

Mouse IgG2b Control
ICIGG2BPP5.5-100 100 µg
EUR 387.6

Mouse IgG2b Control
ICIGG2BPU-100 100 µg
EUR 239.4

CHO Control Antigen
F063 50 ul
EUR 262.8
Description: CHO Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

SF9 Control Antigen
F127 50 ul
EUR 288
Description: SF9 Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

PER.C6 Control Antigen
F536 70 ul
EUR 400.8
Description: PER.C6 Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

FMK Negative Control
1122-100 each
EUR 210

FMK Negative Control
1122-20C each
EUR 210

IgG control Antibody
abx234182-100ug 100 ug
EUR 577.2

IgG control antibody
PAab09882 100 ug
EUR 463.2

pGL3- Control Plasmid
PVT1306 2 ug
EUR 319.2

Mucin (Control Slides)
TCS0016-100 100 Slides
EUR 847.2

Mucin (Control Slides)
TCS0016-25 25 Slides
EUR 278.4

Mucin (Control Slides)
TCS0016-5 5 Slides
EUR 117.6

EUROSTAR 200 control P4 Overhead Stirrer with wireless control unit
STI2882 EACH
EUR 7819.2

TRAF6 Control Peptide
H-7606.0001 1.0mg
EUR 607.2
Description: Sum Formula: C139H232N34O42; CAS# [852690-80-3] net

Fungus (Control Slides)
TCS0005-100 100 Slides
EUR 847.2

Fungus (Control Slides)
TCS0005-25 25 Slides
EUR 278.4

Fungus (Control Slides)
TCS0005-5 5 Slides
EUR 117.6

Hemoglobin A1c control
35R-AH001 1 ml
EUR 307.2
Description: Hemoglobin A1c control

siRNA Negative Control
20-abx941273
  • EUR 260.40
  • EUR 309.60
  • 2.5 nmol
  • 5 nmol

Rabbit Isotype Control
abx200646-100ug 100 ug
EUR 393.6

GFP Lentivirus Control
LTV-300 1 vial
EUR 679.2
Description: HIV-1 Lentivirus

RFP Lentivirus Control
LTV-301 1 vial
EUR 679.2
Description: HIV-1 Lentivirus

Control Magnetic Beads
M1302-1 each Ask for price

Rabbit Isotype Control
IMN-001 100 µg
EUR 239.4

L.lactis Control Antigen
F497 50 ul
EUR 308.4
Description: L.lactis Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

Amyloid (Control Slides)
TCS0003-100 100 Slides
EUR 847.2

Amyloid (Control Slides)
TCS0003-25 25 Slides
EUR 278.4

Amyloid (Control Slides)
TCS0003-5 5 Slides
EUR 117.6

Calcium (Control Slides)
TCS0007-100 100 Slides
EUR 847.2

Calcium (Control Slides)
TCS0007-25 25 Slides
EUR 278.4

Calcium (Control Slides)
TCS0007-5 5 Slides
EUR 117.6

Elastic (Control Slides)
TCS0008-100 100 Slides
EUR 847.2

Elastic (Control Slides)
TCS0008-25 25 Slides
EUR 278.4

Elastic (Control Slides)
TCS0008-5 5 Slides
EUR 117.6

Melanin (Control Slides)
TCS0015-100 100 Slides
EUR 847.2

Melanin (Control Slides)
TCS0015-25 25 Slides
EUR 278.4

Melanin (Control Slides)
TCS0015-5 5 Slides
EUR 117.6

ViaCheck Viability Control 100%
VC50B-20 20 ml
EUR 581.5
Description: ViaCheck Viability Control 100% mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers 

Mouse Lipin-1 Control/blocking peptide control/blocking peptide #1
LPN11-P 100 ug
EUR 196.8

Mouse Lipin-2 Control/blocking peptide control/blocking peptide #1
LPN21-P 100 ug
EUR 196.8

Mouse Lipin-3 Control/blocking peptide control/blocking peptide #1
LPN31-P 100 ug
EUR 196.8

HBC10 Control waterbath
BAT7536 EACH
EUR 5536.8

Recombinant purified HA-tag -Protein (~9 kda) control WB +ve control
HA11-C 100 ul
EUR 343.2

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-2 100 ug
EUR 270

Recombinant purified Myc-tag -Protein (~9 kda) control WB +ve control
MYC11-C 100 ul
EUR 343.2

Reticulum (Control Slides)
TCS0019-100 100 Slides
EUR 847.2

Reticulum (Control Slides)
TCS0019-25 25 Slides
EUR 278.4

Reticulum (Control Slides)
TCS0019-5 5 Slides
EUR 117.6

RESERVOIR CONTROL MODULE
ZFRES00CM EACH
EUR 1266.43

Recombinant purified FLAG tag-Protein (~9 kda) control WB +ve control
FLAG12-C 100 ul
EUR 343.2

Recombinant purified FLAG tag-Protein (~50 kda) control WB +ve control
FLAG13-C 100 ul
EUR 343.2

IKA EUROSTAR 100 control P4 Overhead Stirrer with wireless control unit
STI2890 EACH
EUR 6274.8

Kemptide Negative Control
5-01422 4 x 5mg Ask for price

Recombinant purified his-tag-Protein (~9 kda) control WB +ve control
HISP11-C 100 ul
EUR 343.2

Goat IgG Control Sera
20011-250 0.5 ml
EUR 123.6

NS/0 Control Antigen
F227 50 ul
EUR 308.4
Description: NS/0 Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

Oxidized Glutathione Control, 350UL
C020-350UL 350UL
EUR 85

Compstatin control peptide
B5478-1 1 mg
EUR 447.6

S.cerevisiae Control Antigen
F132 50 ul
EUR 262.8
Description: S.cerevisiae Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

Argentaffin (Control Slides)
TCS0004-100 100 Slides
EUR 847.2

Argentaffin (Control Slides)
TCS0004-25 25 Slides
EUR 278.4

Argentaffin (Control Slides)
TCS0004-5 5 Slides
EUR 117.6

4-Hydroxynonenal (HNE)-BSA protein conjugate for ELISA (in PBS)
HNE15-R 100 ug
EUR 416.4

Mouse IgG Control Sera
20008-250 0.5 ml
EUR 123.6

SP2/0 Control Antigen
F186 50 ul
EUR 308.4
Description: SP2/0 Control Antigen by Cygnus Technologies is available in Europe via Gentaur.

Bielschowsky's (Control Slides)
TCS0006-100 100 Slides
EUR 847.2

Bielschowsky's (Control Slides)
TCS0006-25 25 Slides
EUR 278.4

Bielschowsky's (Control Slides)
TCS0006-5 5 Slides
EUR 117.6

nAb Agarose Control 0.5ml
ABP-NAB-ACNTRL5 500 µl Ask for price

AAV1 Null Control Virus
AAV-351 50 ?L
EUR 1221.6
Description: Null (empty) control virus of AAV serotype 1.

AAV2 Null Control Virus
AAV-352 50 ?L
EUR 1221.6
Description: Null (empty) control virus of AAV serotype 2.

AAV3 Null Control Virus
AAV-353 50 ?L
EUR 1221.6
Description: Null (empty) control virus of AAV serotype 3.

AAV4 Null Control Virus
AAV-354 50 ?L
EUR 1221.6
Description: Null (empty) control virus of AAV serotype 4.

AAV5 Null Control Virus
AAV-355 50 ?L
EUR 1221.6
Description: Null (empty) control virus of AAV serotype 5.

AAV6 Null Control Virus
AAV-356 50 ?L
EUR 1221.6
Description: Null (empty) control virus of AAV serotype 6.

Rabbit IgG Control Sera
20009-1-200 0.5 ml
EUR 123.6

Donkey IgG Control Sera
20015-1-200 0.5 ml
EUR 123.6

Rat IgG Isotype Control
31-AR15 10 mg
EUR 264
Description: Purified Rat IgG Isotype Control

Low control Human Serum
35-S4321H000-L4 10 ml
EUR 139.2
Description: Low control human Serum

Rat IgM Isotype Control
RIGMA-100 100 µg
EUR 846.24

Rat IgM Isotype Control
RIGMB-200 200 µg
EUR 933.6

Rat IgM Isotype Control
RIGMB-50 50 µg
EUR 304.61

Rat IgM Isotype Control
RIGMF-200 200 µg
EUR 933.6

Rat IgM Isotype Control
RIGMF-50 50 µg
EUR 304.61

Rat IgM Isotype Control
RIGMPE-100 100 µg
EUR 549.22

Rat IgM Isotype Control
RIGMPE-25 25 µg
EUR 287.14

Rat IgM Isotype Control
RIGMPP5.5-25 25 µg
EUR 426.91

Rat IgM Isotype Control
RIGMPU-50 50 µg
EUR 217.25

Rat IgM Isotype Control
RIGMPU-500 500 µg
EUR 479.33

Cytotoxicity Control (saponin)
Z5030008 50 mg
EUR 213

nAb Magnetic Control 0.5ml
ABP-NAB-MCNTRL5 500 µl Ask for price

AAV1-GFP Control Virus
AAV-301 50 ?L
EUR 1221.6
Description: GFP control virus of AAV serotype 1.

AAV2-GFP Control Virus
AAV-302 50 ?L
EUR 1221.6
Description: GFP control virus of AAV serotype 2.

AAV3-GFP Control Virus
AAV-303 50 ?L
EUR 1221.6
Description: GFP control virus of AAV serotype 3.

AAV4-GFP Control Virus
AAV-304 50 ?L
EUR 1221.6
Description: GFP control virus of AAV serotype 4.

AAV5-GFP Control Virus
AAV-305 50 ?L
EUR 1221.6
Description: GFP control virus of AAV serotype 5.

AAV6-GFP Control Virus
AAV-306 50 ?L
EUR 1221.6
Description: GFP control virus of AAV serotype 6.

AAV2-Cre Control Virus
AAV-310 50 ?L
EUR 1221.6
Description: Cre control virus of AAV serotype 2.

AAV1-Cre Control Virus
AAV-311 50 ?L
EUR 1221.6
Description: Cre control virus of AAV serotype 1.

AAV3-Cre Control Virus
AAV-313 50 ?L
EUR 1221.6
Description: Cre control virus of AAV serotype 3.

AAV4-Cre Control Virus
AAV-314 50 ?L
EUR 1221.6
Description: Cre control virus of AAV serotype 4.

AAV5-Cre Control Virus
AAV-315 50 ?L
EUR 1221.6
Description: Cre control virus of AAV serotype 5.

AAV6-Cre Control Virus
AAV-316 50 ?L
EUR 1221.6
Description: Cre control virus of AAV serotype 6.

AAV2-Luc Control Virus
AAV-320 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 2.

AAV1-Luc Control Virus
AAV-321 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 1.

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