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Algemeen / Anders / Assay / Isotype

van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel

- June 11, 2021April 30, 2021 - annelies

Kwantitatieve bepaling van sporenmetaal in celkweekmedia met behulp van LS-APGD-MS en ICP-OES met differentiatie van vrije / gebonden soorten na scheidingen van polymeervezels

Liquid sampling-atmospheric pressure glow discharge-mass spectrometry (LS-APGD-MS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES) were employed for the quantification of trace metals in cell culture media and their capabilities compared. The LS-APGD is interfaced here to a compact mass spectrometer (Advion CMS) towards the development of an at-bioreactor process monitoring strategy. Both techniques have been previously employed for the quantification of trace metals in samples of various complexities, making them a natural choice for this application.

They have also demonstrated comparable analytical figures of merit including limits of detection (LOD), matrix tolerance, etc. While cell culture media is a complex sample, the ICP-OES technique was unaffected by the matrix. However, the LS-APGD-MS suffered from increases in spectral background. Despite this, both techniques achieved appropriate LODs for all metals analyzed in this work (Cu, Fe, Zn, Co, Mn, Ni; LOD < 100 ng mL-1), except for Mn and Ni via LS-APGD-MS. To overcome the increased background seen on the LS-APGD-MS, a capillary channeled polymer (C-CP) polypropylene (PPY) fiber stationary phase was employed as an on-line separation for the removal of organic components prior to sample introduction into the plasma.
It was further determined that Ni was retained on the column, preventing the detection of this element via LS-APGD-MS, and insights into metal speciation were discussed. Following implementation of this on-line separation strategy, the agreement between the techniques was acceptable for all analytes, and was excellent for Cu, Fe, and Zn.

Co-gereguleerde pilus van vibrio cholerae-toxine veroorzaakt ontstekingsreacties in een co-cultuurmodel van Caco-2 en perifere bloedmononucleaire cellen (PBMC), wat leidt tot verhoogde kolonisatie

The aim of this study was to assess the modulatory effect of TcpA in the expression of CEACAM1 adhesin molecule and IL-1, IL-8 and TNFα pro-inflammatiory cytokines in the co-culture model of Caco-2/PBMC that can mimic the intestinal milieu. The tcpA gene from V.cholerae ATCC14035 was cloned in pET-28a and transformed into E.coli Bl-21. The recombinant TcpA-His6 protein was expressed and purified using the Ni-column chromatography.
The sequencing of transformed plasmid and Western blotting of purified protein confirmed the identity of rTcp. Cytotoxicity of different concentrations of recombinant protein for human colon carcinoma cell line (Caco-2 cell) was assessed by MTT assay and showed viability of 92%, 82% and 70 %, for10 µg/mL of TcpA after 24, 48 and 72 h, respectively. Co-cultures of Caco-2 and peripheral blood mononuclear cells (PBMCs) were used to mimic the intestinal milieu and treated with different concentrations of rTcpA (1, 5, 10 and 50 µg/mL).
Our data showed about 2.04, 3.37, 3.68 and 42.7-folds increase in CEACAM1 gene expression, respectively, compared with non-treated Caco-2/PBMC co-culture. Moreover, the expression of IL-1, IL-8 and TNFα genes was significantly increased up to 15.75, 7.04 and 80.95 folds, respectively. In conclusion, V.cholerae TcpA induces statistically significant dose-dependent stimulatory effect on TNFα, IL-1 and IL-8 pro-inflammatory cytokines expression. Among all, TNFα was much more affected which consequently, elevated CEACAM1 expression level in IECs. This suggests TcpA protein as critical effector as an inducer of increased adhesion potential of V. cholera as well as inflammatory responses of host intestinal tissue. This article is protected by copyright. All rights reserved.
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Een nieuwe isolatie- en kweekmethode voor granulosacellen

Granulosa cells are the cell population who have an increasing importance in the female genital system and reproduction. Thus, nowadays in vitro studies to address these cells are also gaining importance and attracts researcher’s attention. The aim of our study is to develop a more feasible, low-cost granulosa cell isolation and culture method compared to methods defined so far.
Granulosa cells were isolated from follicular fluids obtained from both healthy women donors (n = 19) and polycystic ovary syndrome (n = 15) applied to in vitro fertilization treatment process. Granulosa cells were isolated by using Lymphosep® separation fluid that was not used for this purpose before. The isolated cells were cultured in suitable culture dishes with a mixture of BIO-AMFTM-1 and DMEM/F12 in the first seeding and only complete DMEM/F12 in the following feeds.
  • The complete medium contains only 5% fetal calf serum, 4% L-glutamine and 1% penicillin-streptomycin-amphotericin. The new methods we have developed in granulosa cell isolation and in vitro culture have been successful.
  • Reduction in supplement types and amount; improved the proliferation rate of the granulosa cells in culture. Our new methods of isolation and cell culture for granulosa cells from healthy women, have been also successful in samples of polycystic ovarian patients.
  • With these developed methods, granulosa cells, which belong to humans and have an important role in the ovary, could be isolated and subsequently be maintained to reproduce (proliferate) more easily and cheaper.

Over de oorsprong van SARS-CoV-2: hebben celkweekexperimenten geleid tot verhoogde virulentie van het voorlopervirus voor mensen?

We are currently in a rapidly expanding pandemic of the SARS-CoV-2 virus, which originated in the city of Wuhan in central China. The disease COVID-19 is now spread worldwide and has tremendous socio-economic consequences. The origin of the virus can be reconstructed through epidemiological studies and, even more so, from genome comparisons. How the evolution of the virus and the transition to humans might have happened is the subject of much speculation.
It is considered certain that the virus is of animal origin and very likely passed from bats to humans in a zoonotic event. An intermediate host was postulated, but many SARS-like bat viruses have the ability to infect human cells directly, which has been shown experimentally by scientists in the Wuhan Institute of Virology using collected specimens containing virus material from horseshoe bats.
The propagation of SARS-like bat viruses in cell culture allowed experiments aimed at increasing the infectivity of the virus and adaptation to human cells. This article summarizes the unique properties of SARS-CoV-2 and focusses on a specific sequence encoding the spike protein.
Possible scenarios of virus evolution are discussed, with particular emphasis on the hypothesis that the virus could have emerged unintentionally through routine culture or gain-of-function experiments in a laboratory, where it was optimally adapted to human cells and caused cryptic infections among workers who finally spread the virus causing the pandemic.

 

BSA Control for Age-BSA
2221-BSA
EUR 175

Cotinine-BSA Conjugate
COTN15-BSA 0.5 mg
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Positive control tissue section for each antibody; Based on availability INQUIRE
Control-Slides Set of 5
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BSA Control for AGE-BSA
35R-AA007 10 mg
EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

CEL-BSA Control
STA-302 100 µg
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Description:
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STA-306 100 µg
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Description:
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STA-314 100 µg
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Bovine Serum Albumin Biotinylated (Biotin-BSA)
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Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate
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HNE antibody
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EUR 300
Description: Goat polyclonal HNE antibody

4-Hydroxynonenal (HNE)-BSA protein conjugate for ELISA (in PBS)
HNE15-R 100 ug
EUR 347

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Bovine Serum Albumin (BSA) ELISA Kit
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Bovine Serum Albumin (BSA) ELISA Kit
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Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)
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Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)
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Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE)
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General Bovine Serum Albumin (BSA) ELISA Kit
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HNE13-M 100 ul
EUR 482

4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)
HNE11-C 100 ul
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4-Hydroxynonenal (HNE)-BSA protein conjugate for Western blots (in sample buffer)
HNE12-C 100 ul
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Polyclonal HNE / Neutrophil Elastase Antibody
APS00008G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Sheep that recognizes and binds to Human HNE / Neutrophil Elastase . This antibody is tested and proven to work in the following applications:

Human 4-HNE ELISA Kit
EHH0210 96Tests
EUR 521

Goat 4-HNE ELISA Kit
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Bovine 4-HNE ELISA Kit
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4-Hydroxynonenal (4-HNE) Antibody
abx411903-01ml 0.1 ml
EUR 509
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4 hydroxynonenal (HNE), >98% pure
HNE51-1 1 mg
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4 hydroxynonenal (HNE), >98% pure
HNE51-5 5 mg
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Rat 4-HNE ELISA Kit
ERH0210 96Tests
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Sheep 4-HNE ELISA Kit
ESH0210 96Tests
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ERTH0210 96Tests
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EMH0210 96Tests
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EPH0210 96Tests
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anti-4-HNE (HNEJ-2)
LF-MA90010 20 ug
EUR 360
Description: Mouse monoclonal to 4-HNE

anti-4-HNE (HNEJ-2)
LF-MA90011 100 ug
EUR 891
Description: Mouse monoclonal to 4-HNE

Malondialdehyde (MDA)-BSA Conjugate positive control for Western/ELISA
MDA35-N-100 100 ug
EUR 286

Pre-diluted BSA (bovine serum albumin) standards (250, 125, 62.5, 31.25, and 0.0 ug/ml) for BCA Optima Protein Assay kit
BCA-BSA-2 1 pk
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Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column
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800-225-BSA 1 kit
EUR 2425

Rat 4-Hydroxynonenal(HNE)ELISA Kit
CSB-EQ027232RA-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Rat 4-Hydroxynonenal (HNE) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat 4-Hydroxynonenal(HNE)ELISA Kit
1-CSB-EQ027232RA
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Rat 4-Hydroxynonenal(HNE) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

4-Hydroxynonenal (4-HNE) ELISA Kit
abx257639-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.

Guinea Pig 4-HNE ELISA Kit
EGH0210 96Tests
EUR 521

4-Hydroxynonenal (4-HNE) ELISA Kit
E4645-100
EUR 865

Mouse 4-Hydroxynonenal (HNE)ELISA Kit
CSB-E13412m-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse 4-Hydroxynonenal (HNE) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse 4-Hydroxynonenal (HNE)ELISA Kit
1-CSB-E13412m
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse 4-Hydroxynonenal (HNE) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human 4-Hydroxynonenal (HNE)ELISA Kit
CSB-E16214h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Human 4-Hydroxynonenal (HNE) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human 4-Hydroxynonenal (HNE)ELISA Kit
1-CSB-E16214h
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Human 4-Hydroxynonenal (HNE) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit Anti-4 hydroxynonenal (HNE) antiserum
HNE11-S 100 ul
EUR 457

OxiSelect HNE Adduct Competitive ELISA Kit
STA-838 96 assays
EUR 798
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

OxiSelect HNE Adduct Competitive ELISA Kit
STA-838-5 5 x 96 assays
EUR 3240
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

ELISA kit for 4-HNE (4-Hydroxynonenal)
E-EL-0128 1 plate of 96 wells
EUR 377
  • Gentaur's 4-HNE ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid pha
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Description: A competitive ELISA kit for quantitative measurement of 4-HNE (4-Hydroxynonenal) in samples from Serum, Plasma, Cell supernatant

Mouse 4-Hydroxynonenal (4-HNE) ELISA Kit
abx257660-96tests 96 tests
EUR 754
  • Shipped within 5-12 working days.

Human 4-Hydroxynonenal (4-HNE) ELISA Kit
abx251411-96tests 96 tests
EUR 754
  • Shipped within 5-12 working days.

Human 4-HNE(4-Hydroxynonenal) ELISA Kit
EH2080 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
  • Alias: 4-HNE(4-Hydroxynonenal)/HNE/4 HNE
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 pg/ml

Goat Anti-4 hydroxynonenal (HNE) antiserum #2
HNE12-S 100 ul
EUR 457

Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit
EM1583 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Rat 4-HNE(4-Hydroxynonenal) ELISA Kit
ER1587 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 18.75 pg/ml

Goat Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody
STA-034 100 µg
EUR 664
Description: Goat Anti-4-hydroxynonenal Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

Rabbit Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody
STA-035 100 µg
EUR 664
Description: Rabbit Anti-4-hydroxynonenal Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

BSA
31C-CH0114 50 mg
EUR 133
Description: Bovine Serum Albumin for use in the research laboratory

Testosterone 3 BSA Protein (BSA)
abx060856-1mg 1 mg
EUR 578
  • Shipped within 5-10 working days.

Ibrutinib drug-Human Serum Albumin Conjugate
IBT16-BSA 100 ug
EUR 529

Mouse (mrl/lpr) serum containing autoantibodies to HNE
HNE31-M 100 ul
EUR 482

OxiSelect HNE Adduct Competitive ELISA Kit, Trial Size
STA-838-T 32 assays
EUR 421
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

Control siRNA Vector (pGB-control)
9500C-20
EUR 338

Histamine [G-BSA] (G-G-BSA)
DAG3327 1mg
EUR 1234

Cyclic citrullinated peptide-BSA（CCP-BSA）
E64I00802 100ug
EUR 343

AGE-BSA
2221-10
EUR 370

AGE-BSA
2221-50 Ask for price

BSA antibody
20R-1871 100 ug
EUR 673
Description: Rabbit polyclonal BSA antibody

BSA antibody
20R-AR042 20 mg
EUR 327
Description: Rabbit polyclonal BSA antibody

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