fondationvocation

Differentiatie van door de mens geïnduceerde pluripotente stamcellen

3D-cultuurmodellen als drugtestplatforms bij hondenlymfoom en hun overspraak met lymfeklier-afgeleide stromacellen

Background: Malignant lymphoma is the most common hematopoietic malignancy in dogs, and relapse is frequently seen despite aggressive initial treatment. In order for the treatment of these recurrent lymphomas in dogs to be effective, it is important to choose a personalized and sensitive anticancer agent. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key characteristics of the original tumor.
Objectives: In this study, we established a model of hybrid tumor/stromal spheroids and investigated the association between canine lymphoma cell line (GL-1) and canine lymph node (LN)-derived stromal cells (SCs).
Methods: A hybrid spheroid model consisting of GL-1 cells and LN-derived SC was created using ultra low attachment plate. The relationship between SCs and tumor cells (TCs) was investigated using a coculture system.
Results: TCs cocultured with SCs were found to have significantly upregulated multidrug resistance genes, such as P-qpMRP1, and BCRP, compared with TC monocultures. Additionally, it was revealed that coculture with SCs reduced doxorubicin-induced apoptosis and G2/M cell cycle arrest of GL-1 cells.
Conclusions: SCs upregulated multidrug resistance genes in TCs and influenced apoptosis and the cell cycle of TCs in the presence of anticancer drugs. This study revealed that understanding the interaction between the tumor microenvironment and TCs is essential in designing experimental approaches to personalized medicine and to predict the effect of drugs.
Keywords: Canine lymphoma; drug resistance; lymph node-derived stromal cell; personalized cancer therapy; tumor microenvironment.

Het overwinnen van kweekbeperking voor SARS-CoV-2 in menselijke cellen vergemakkelijkt het screenen van verbindingen die virale replicatie remmen

Efforts to mitigate the COVID-19 pandemic include screening of existing antiviral molecules that could be re-purposed to treat SARS-CoV-2 infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nucs) often show decreased activity in these cells due to inefficient metabolization. SARS-CoV-2 exhibits low viability in human cells in culture.
Here, serial passages of a SARS-CoV-2 isolate (original-SARS2) in the human hepatoma cell clone Huh7.5 led to the selection of a variant (adapted-SARS2) with significantly improved infectivity in human liver (Huh7 and Huh7.5) and lung cancer cells (unmodified Calu-1 and A549). The adapted virus exhibited mutations in the spike protein, including a 9 amino acid deletion and 3 amino acid changes (E484D, P812R, and Q954H). E484D also emerged in Vero E6 cultured viruses that became viable in A549 cells.
Original and adapted viruses were susceptible to SR-B1 receptor blocking and adapted-SARS2 exhibited significantly less dependency of ACE2. Both variants were similarly neutralized by COVID-19 convalescent plasma but adapted-SARS2 exhibited increased susceptibility to exogenous type I interferon. Remdesivir inhibited original- and adapted-SARS2 similarly, demonstrating the utility of the system for the screening of nucs.
Among the tested nucs, only remdesivir, molnupiravir and to a limited extent galidesivir, showed antiviral effect across human cell lines, whereas sofosbuvir, ribavirin, and favipiravir had no apparent activity. Analogously to the emergence of spike mutations in vivo, the spike protein is under intense adaptive selection pressure in cell culture. Our results indicate that the emergence of spike mutations will most likely not affect the activity of remdesivir.
fondationvocation
fondationvocation

H-Ras Activation Assay Kit, Trial Size

STA-400-H-T 5 assays
EUR 403
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

K-Ras Activation Assay Kit, Trial Size

STA-400-K-T 5 assays
EUR 403
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

N-Ras Activation Assay Kit, Trial Size

STA-400-N-T 5 assays
EUR 403
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

Rac1 Activation Assay

STA-401-1 20 assays
EUR 757
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rac2 Activation Assay

STA-401-2 20 assays
EUR 757
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Cdc42 Activation Assay

STA-402 20 assays
EUR 757
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

RhoA Activation Assay

STA-403-A 20 assays
EUR 757
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoB Activation Assay

STA-403-B 20 assays
EUR 757
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay

STA-403-C 20 assays
EUR 757
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Arf1 Activation Assay

STA-407-1 20 assays
EUR 757
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Arf6 Activation Assay

STA-407-6 20 assays
EUR 757
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Ral Activation Assay

STA-408 20 assays
EUR 757
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay

STA-409 20 assays
EUR 757
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

Pan-RAS-IN-1

HY-101295 25mg
EUR 1703

Rap1 Activation Assay Kit

STA-406-1 20 assays
EUR 757
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rap2 Activation Assay Kit

STA-406-2 20 assays
EUR 757
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rac1 Activation Assay Kit, Trial Size

STA-401-1-T 5 assays
EUR 403
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rac2 Activation Assay Kit, Trial Size

STA-401-2-T 5 assays
EUR 403
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Cdc42 Activation Assay Kit, Trial Size

STA-402-T 5 assays
EUR 403
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

RhoA Activation Assay Kit, Trial Size

STA-403-A-T 5 assays
EUR 403
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoB Activation Assay Kit, Trial Size

STA-403-B-T 5 assays
EUR 403
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay Kit, Trial Size

STA-403-C-T 5 assays
EUR 403
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Rac1/Cdc42 Activation Assay Combo Kit

STA-404 2 x 20 assays
EUR 1088
Description: Our Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit.

Rap1 Activation Assay Kit, Trial Size

STA-406-1-T 5 assays
EUR 403
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rap2 Activation Assay Kit, Trial Size

STA-406-2-T 5 assays
EUR 403
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Arf1 Activation Assay Kit, Trial Size

STA-407-1-T 5 assays
EUR 403
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Arf6 Activation Assay Kit, Trial Size

STA-407-6-T 5 assays
EUR 403
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Ral Activation Assay Kit, Trial Size

STA-408-T 5 assays
EUR 403
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay Kit, Trial Size

STA-409-T 5 assays
EUR 403
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

RhoA/Rac1/CDC42 Activation Assay Combo Kit

STA-405 3 x 10 assays
EUR 1088
Description: Our RhoA/Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit.

96-Well Ras Activation ELISA Kit (Colorimetric)

STA-440 96 assays
EUR 902
Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.

96-Well Ras Activation ELISA Kit (Chemiluminescent)

STA-441 96 assays
EUR 902
Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.

Pan-PAX (Pan-PAX) Antibody

abx236136-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Cytokeratin, pan (Epithelial Marker); Clone PAN-CK (Concentrate)

RA0430-C.5 0.5 ml
EUR 300

pan-Cytokeratin antibody

23032-100ul 100ul
EUR 390

Keratin pan antibody

20R-2530 250 uL
EUR 510
Description: Rabbit polyclonal Keratin pan antibody

Synuclein pan antibody

70R-30766 100 ug
EUR 327
Description: Rabbit polyclonal Synuclein pan antibody

Actin pan antibody

70R-31095 100 ug
EUR 327
Description: Rabbit polyclonal Actin pan antibody

H3 Pan Antibody

6806-50
EUR 316

Synuclein-pan Antibody

33495-100ul 100ul
EUR 252

Synuclein-pan Antibody

33495-50ul 50ul
EUR 187

pan-AKT Antibody

36716-100ul 100ul
EUR 252

pan CDH Antibody

37301-100ul 100ul
EUR 252

pan-AKT antibody

38602-100ul 100ul
EUR 252

Actin-pan Antibody

33321-100ul 100ul
EUR 252

Actin-pan Antibody

33321-50ul 50ul
EUR 187

pan Cytokeratin antibody

10R-2060 100 ul
EUR 403
Description: Mouse monoclonal pan Cytokeratin antibody

Cytokeratin Pan antibody

10R-2096 100 ul
EUR 403
Description: Mouse monoclonal Cytokeratin Pan antibody

pan Keratin antibody

10R-2442 50 ug
EUR 405
Description: Mouse monoclonal pan Keratin antibody

LDH Pan antibody

10-1335 1 mg
EUR 259
Description: Mouse monoclonal LDH Pan antibody

LDH Pan antibody

10-1336 1 mg
EUR 259
Description: Mouse monoclonal LDH Pan antibody

pan Endothelium antibody

10R-6386 100 ug
EUR 284
Description: Mouse monoclonal pan Endothelium antibody

pan Cadherin antibody

10R-7890 100 ug
EUR 322
Description: Mouse monoclonal pan Cadherin antibody

Actin-pan antibody

10R-A106a 100 ug
EUR 553
Description: Mouse monoclonal Actin-pan antibody

Pan-Cadherin Antibody

48907-100ul 100ul
EUR 333

Pan-Cadherin Antibody

48907-50ul 50ul
EUR 239

pan Cadherin Antibody

45029-100ul 100ul
EUR 252

pan Cadherin Antibody

45029-50ul 50ul
EUR 187

pan Cadherin Antibody

45052-100ul 100ul
EUR 252

pan Cadherin Antibody

45052-50ul 50ul
EUR 187

Pan myristoylation Antibody

1-CSB-PA885152
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Description: A polyclonal antibody against Pan myristoylation. Recognizes Pan myristoylation from ALL. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-10000, ELISA:1:10000

pan Cadherin Antibody

DF2942 200ul
EUR 304
Description: pan Cadherin Antibody detects endogenous levels of total pan Cadherin.

pan-AKT Antibody

DF7208 200ul
EUR 304
Description: pan-AKT Antibody detects endogenous levels of total pan-AKT.

pan Cadherin Antibody

DF7514 200ul
EUR 304
Description: pan Cadherin Antibody detects endogenous levels of total pan Cadherin.

Pan Methylated Lysine

DF7728 200ul
EUR 304
Description: Pan Methylated Lysine Rabbit pAbdetects endogenous levels of total Pan Methylated Lysine Rabbit pAb.

Pan myristoylation Antibody

1-CSB-PA465729
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Description: A polyclonal antibody against Pan myristoylation. Recognizes Pan myristoylation from ALL. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-10000, ELISA:1:10000

FOXO1 pan antibody

70R-37057 100 ug
EUR 327
Description: Rabbit Polyclonal FOXO1 pan antibody

SSX pan antibody

70R-50426 100 ul
EUR 244
Description: Purified Polyclonal SSX pan antibody

NBPF pan antibody

70R-51031 100 ul
EUR 244
Description: Purified Polyclonal NBPF pan antibody

Melanoma; Pan (Concentrate)

A00134-C 1 ml
EUR 676

Melanoma; Pan (Concentrate)

A00134-C.1 0.1 ml
EUR 138

PKC pan antibody

70R-33408 100 ug
EUR 327
Description: Rabbit polyclonal PKC pan antibody

NBPF-pan Antibody

20-abx008107
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

SSX-pan Antibody

20-abx008681
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Pan pLDH Antibody

abx023860-1mg 1 mg
EUR 342
  • Shipped within 5-10 working days.

Pan pLDH Antibody

abx023861-1mg 1 mg
EUR 342
  • Shipped within 5-10 working days.

Pan SUMO Antibody

abx027085-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Pan SUMO Antibody

abx027085-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Pan SUMO Antibody

abx027140-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Pan SUMO Antibody

abx027140-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

pan Cadherin Antibody

abx217630-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

Pan-AKT Antibody

20-abx125375
  • EUR 592.00
  • EUR 857.00
  • EUR 411.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

pan Cadherin Antibody

20-abx149140
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Actin-pan Antibody

20-abx013019
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

Synuclein-pan Antibody

20-abx013202
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

Actin-pan Antibody

AF0115 200ul
EUR 304
Description: Actin-pan antibody detects endogenous levels of total Actin-pan.

PKC-pan Antibody

AF6197 200ul
EUR 304
Description: PKC-pan Antibody detects endogenous levels of total PKC-pan.

Pan myristoylation Antibody

20-abx322788
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Pan myristoylation Antibody

20-abx322789
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Pan Ubiquitin Antibody

abx415347-50ug 50 ug
EUR 565
  • Shipped within 1 week.

pan ADH Antibody

abx433090-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

SHANK (pan) Antibody

abx440129-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx440140-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx440246-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx440410-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx440421-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx440527-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx440691-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx440702-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx440808-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx440972-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx440983-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx441089-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx441253-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx441264-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx441370-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx441534-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx441545-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx441651-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx441815-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx441826-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx441932-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx442096-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx442107-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx442213-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx442377-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx442388-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx442494-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx442658-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx442669-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx442775-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx442938-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx442949-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx443055-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx443218-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx443229-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx443335-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx443499-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx443510-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx443616-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx443780-100ug 100 ug
EUR 592
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx443791-100ug 100 ug
EUR 592
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx443897-100ug 100 ug
EUR 592
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx444061-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx444072-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx444178-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx444342-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx444353-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx444459-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx444623-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx444634-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx444740-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

SHANK (pan) Antibody

abx444980-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

SAPAP (pan) Antibody

abx444991-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

GRK (pan) Antibody

abx445060-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

QKI (pan) Antibody

abx445076-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

Ankyrin (pan) Antibody

abx445097-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

PKC- pan Antibody

ABF6197 100 ug
EUR 438

pan Cadherin Antibody

ABD2942 100 ug
EUR 438

pan- AKT Antibody

ABD7208 100 ug
EUR 438

pan Cadherin Antibody

ABD7514 100 ug
EUR 438

Actin- pan Antibody

ABF0115 100 ug
EUR 438

Actin- pan Antibody

ABF5288 100 ug
EUR 438

pan-AKT Antibody

20-abx002260
  • EUR 411.00
  • EUR 592.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

anti-pan-Synuclein

LF-PA0194 100 ul
EUR 334
Description: Rabbit polyclonal to pan-Synuclein

Pan Keratin antibody

PAab09935 100 ug
EUR 386

pan Akt/KinaseB

MO15107 100 ug
EUR 435

NMDA NR1 Pan

MO25041 15 ug
EUR 592

RAS Antibody

31153-100ul 100ul
EUR 252

RAS Antibody

31153-50ul 50ul
EUR 187

RAS antibody

10R-1779 100 ul
EUR 349
Description: Mouse monoclonal RAS antibody

Ras Antibody

48578-100ul 100ul
EUR 333

Ras Antibody

48578-50ul 50ul
EUR 239

Ras Antibody

48602-100ul 100ul
EUR 333

Ras Antibody

48602-50ul 50ul
EUR 239

Ras Antibody

49408-100ul 100ul
EUR 333

Ras Antibody

49408-50ul 50ul
EUR 239

Rabbit Anti-Llama IgG (H+L chain), Antisera (Bulk)

30818-RAS-BLK 1-10L, bulk Ask for price

Rabbit Anti-Human IgG, Antisera (Bulk)

10119-RAS-BLK 1-10L, bulk Ask for price

Rabbit Anti-Goat IgG, Antisera (Bulk)

60319-RAS-BLK 1-10L, bulk Ask for price

Rabbit Anti-Mouse IgG, Antisera (Bulk)

44319-RAS-BLK 1-10L, bulk Ask for price

XTT CELL VIABILITY ASSAY KIT (1000 ASSAY)

30007 1KIT
EUR 236
Description: Minimum order quantity: 1 unit of 1KIT

Ras-Related Protein R-Ras (RRAS) Antibody

abx026316-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Ras-Related Protein R-Ras (RRAS) Antibody

abx026316-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Ras-related protein R-Ras (RRAS) Antibody

20-abx214133
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Ras-Related Protein M-Ras (MRAS) Antibody

20-abx113895
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Ras-Related Protein R-Ras (RRAS) Antibody

20-abx123552
  • EUR 495.00
  • EUR 356.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Ras-related protein R-Ras (RRAS) Antibody

20-abx241995
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Ras-Related Protein R-Ras (RRAS) Antibody

abx237489-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Ras-Related Protein R-Ras (RRAS) Protein

20-abx262883
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)

CBA-200 50 assays
EUR 560
Description: For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor cells. These cells proliferate to form new blood vessels. Our Endothelial Tube Formation Assay (In Vitro Angiogenesis) provides an easy, robust system to assess angiogenesis in vitro. The ECM gel matrix very closely resembles an in vivo environment.

Monoclonal Cytokeratin, pan (Epithelial Marker) Antibody, Clone: PAN-CK (Cocktail)

APR14636G 7 ml
EUR 484
Description: A Monoclonal antibody against Human Cytokeratin, pan (Epithelial Marker). The antibodies are raised in Mouse and are from clone PAN-CK (Cocktail). This antibody is applicable in WB, IHC and IF, FC

Pan Acetyl-Lysine pAb

30416-100ul 100ul
EUR 252

Pan Acetyl-Lysine pAb

30416-50ul 50ul
EUR 187

Pan DiMethyl-lysine pAb

30669-100ul 100ul
EUR 252

Pan DiMethyl-lysine pAb

30669-50ul 50ul
EUR 187

Pan hair keratins antibody

20R-2654 100 uL
EUR 571
Description: Guinea Pig polyclonal pan hair keratins antibody

E cadherin pan antibody

70R-31124 100 ug
EUR 327
Description: Rabbit polyclonal E cadherin pan antibody

Anti- Myosin-pan Antibody

A1213-100
EUR 403

Anti-Keratin-pan Antibody

A05667 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Keratin-pan Antibody (KRT2) detection.tested for IHC, WB in Human, Mouse, Rat.

Sodium Channel-pan Antibody

33488-100ul 100ul
EUR 252

Sodium Channel-pan Antibody

33488-50ul 50ul
EUR 187

Rat Pan Granulocytes antibody

10R-G123a 75 ug
EUR 637
Description: Mouse monoclonal Rat Pan Granulocytes antibody

Mouse Pan Macrophages antibody

10R-M156a 200 ug
EUR 975
Description: Rat monoclonal Mouse Pan Macrophages antibody

Mouse Pan Macrophages antibody

10R-M156b 100 ug
EUR 705
Description: Rat monoclonal Mouse Pan Macrophages antibody

In vitro differentiatie van menselijke pluripotente stamcellen in de B-lijn met behulp van OP9-MS5 co-cultuur

 

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia.
Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).

Differentiatie van door de mens geïnduceerde pluripotente stamcellen tot definitief endoderm met behulp van een eenvoudig dialysecultuurapparaat

Therapeutic use of differentiated organ cells from human induced pluripotent stem cells (hiPSCs) is one of the promising strategies for regenerative medicine. Differentiation into definitive endoderm is an essential process in the preparation of metabolic organs. However, the manufacturability of differentiation is limited due to the high-cost cytokines required for the differentiation of endodermal lineage.
Furthermore, the cytokines remaining in the used culture medium and possible endogenous factors are removed along with toxic metabolites by the medium replacement. To address these problems, the application of dialysis culture can retain and fully utilize their accumulation to create a better culture environment that contributes to differentiation cost reduction.
The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0. Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic. We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.
In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and disialylated structures (SI:0.09) of which 75% of sialic acid was Neu5Gc. The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%). Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).
However, the effects on sialylation differed between the two Mabs. We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.
On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc. The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.
However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements. Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells. This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.

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