fondationvocation

Differentiatie van door de mens geïnduceerde pluripotente stamcellen

3D-cultuurmodellen als drugtestplatforms bij hondenlymfoom en hun overspraak met lymfeklier-afgeleide stromacellen

Background: Malignant lymphoma is the most common hematopoietic malignancy in dogs, and relapse is frequently seen despite aggressive initial treatment. In order for the treatment of these recurrent lymphomas in dogs to be effective, it is important to choose a personalized and sensitive anticancer agent. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key characteristics of the original tumor.
Objectives: In this study, we established a model of hybrid tumor/stromal spheroids and investigated the association between canine lymphoma cell line (GL-1) and canine lymph node (LN)-derived stromal cells (SCs).
Methods: A hybrid spheroid model consisting of GL-1 cells and LN-derived SC was created using ultra low attachment plate. The relationship between SCs and tumor cells (TCs) was investigated using a coculture system.
Results: TCs cocultured with SCs were found to have significantly upregulated multidrug resistance genes, such as P-qpMRP1, and BCRP, compared with TC monocultures. Additionally, it was revealed that coculture with SCs reduced doxorubicin-induced apoptosis and G2/M cell cycle arrest of GL-1 cells.
Conclusions: SCs upregulated multidrug resistance genes in TCs and influenced apoptosis and the cell cycle of TCs in the presence of anticancer drugs. This study revealed that understanding the interaction between the tumor microenvironment and TCs is essential in designing experimental approaches to personalized medicine and to predict the effect of drugs.
Keywords: Canine lymphoma; drug resistance; lymph node-derived stromal cell; personalized cancer therapy; tumor microenvironment.

Het overwinnen van kweekbeperking voor SARS-CoV-2 in menselijke cellen vergemakkelijkt het screenen van verbindingen die virale replicatie remmen

Efforts to mitigate the COVID-19 pandemic include screening of existing antiviral molecules that could be re-purposed to treat SARS-CoV-2 infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nucs) often show decreased activity in these cells due to inefficient metabolization. SARS-CoV-2 exhibits low viability in human cells in culture.
Here, serial passages of a SARS-CoV-2 isolate (original-SARS2) in the human hepatoma cell clone Huh7.5 led to the selection of a variant (adapted-SARS2) with significantly improved infectivity in human liver (Huh7 and Huh7.5) and lung cancer cells (unmodified Calu-1 and A549). The adapted virus exhibited mutations in the spike protein, including a 9 amino acid deletion and 3 amino acid changes (E484D, P812R, and Q954H). E484D also emerged in Vero E6 cultured viruses that became viable in A549 cells.
Original and adapted viruses were susceptible to SR-B1 receptor blocking and adapted-SARS2 exhibited significantly less dependency of ACE2. Both variants were similarly neutralized by COVID-19 convalescent plasma but adapted-SARS2 exhibited increased susceptibility to exogenous type I interferon. Remdesivir inhibited original- and adapted-SARS2 similarly, demonstrating the utility of the system for the screening of nucs.
Among the tested nucs, only remdesivir, molnupiravir and to a limited extent galidesivir, showed antiviral effect across human cell lines, whereas sofosbuvir, ribavirin, and favipiravir had no apparent activity. Analogously to the emergence of spike mutations in vivo, the spike protein is under intense adaptive selection pressure in cell culture. Our results indicate that the emergence of spike mutations will most likely not affect the activity of remdesivir.
fondationvocation
fondationvocation

Rac1 Activation Assay

STA-401-1 20 assays
EUR 908.4
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rac2 Activation Assay

STA-401-2 20 assays
EUR 908.4
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

RhoA Activation Assay

STA-403-A 20 assays
EUR 908.4
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoB Activation Assay

STA-403-B 20 assays
EUR 908.4
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay

STA-403-C 20 assays
EUR 908.4
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Arf1 Activation Assay

STA-407-1 20 assays
EUR 908.4
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Arf6 Activation Assay

STA-407-6 20 assays
EUR 908.4
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Cdc42 Activation Assay

STA-402 20 assays
EUR 908.4
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

Rap1 Activation Assay Kit

STA-406-1 20 assays
EUR 908.4
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rap2 Activation Assay Kit

STA-406-2 20 assays
EUR 908.4
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Ral Activation Assay Kit, Trial Size

STA-408-T 5 assays
EUR 483.6
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay Kit, Trial Size

STA-409-T 5 assays
EUR 483.6
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

Rac1 Activation Assay Kit, Trial Size

STA-401-1-T 5 assays
EUR 483.6
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rac2 Activation Assay Kit, Trial Size

STA-401-2-T 5 assays
EUR 483.6
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

RhoA Activation Assay Kit, Trial Size

STA-403-A-T 5 assays
EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoB Activation Assay Kit, Trial Size

STA-403-B-T 5 assays
EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay Kit, Trial Size

STA-403-C-T 5 assays
EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Rap1 Activation Assay Kit, Trial Size

STA-406-1-T 5 assays
EUR 483.6
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rap2 Activation Assay Kit, Trial Size

STA-406-2-T 5 assays
EUR 483.6
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Arf1 Activation Assay Kit, Trial Size

STA-407-1-T 5 assays
EUR 483.6
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Arf6 Activation Assay Kit, Trial Size

STA-407-6-T 5 assays
EUR 483.6
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Cdc42 Activation Assay Kit, Trial Size

STA-402-T 5 assays
EUR 483.6
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

Rac1/Cdc42 Activation Assay Combo Kit

STA-404 2 x 20 assays
EUR 1305.6
Description: Our Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit.

RhoA/Rac1/CDC42 Activation Assay Combo Kit

STA-405 3 x 10 assays
EUR 1305.6
Description: Our RhoA/Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit.

96-Well Ras Activation ELISA Kit (Colorimetric)

STA-440 96 assays
EUR 1082.4
Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.

96-Well Ras Activation ELISA Kit (Chemiluminescent)

STA-441 96 assays
EUR 1082.4
Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.

CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE

354730 5/pk
EUR 102
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-MOUSE CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE

354720 5/pk
EUR 576
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-HUMAN CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE

354725 5/pk
EUR 568.8
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

Stat3 Activation Inhibitor, SPI

1813-2 each
EUR 274.8

CD3 (activation epitope) Antibody

abx139107-01mg 0.1 mg
EUR 460.8

GAL4 Activation Domain Antibody

20-abx330273
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

NF-?B Activation Inhibitor III

C3559-25 25 mg
EUR 548.4
Description: NF-?B Activation Inhibitor III is a NF-?B inhibitor.NF-?B (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex controling transcription of DNA, cytokine production as well as cell survival.

NF-?B Activation Inhibitor III

C3559-5 5 mg
EUR 174
Description: NF-?B Activation Inhibitor III is a NF-?B inhibitor.NF-?B (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex controling transcription of DNA, cytokine production as well as cell survival.

CD11b activation epitope Antibody

abx140366-01mg 0.1 mg
EUR 427.2

CD11b activation epitope Antibody

abx140396-01mg 0.1 mg
EUR 427.2

CD3 (activation epitope) Antibody (PE)

abx139119-01mg 0.1 mg
EUR 577.2

EZAct? Procathepsin Activation Buffer

1030-1 each
EUR 183.6

CD3 activation epitope Antibody (APC)

abx140478-01mg 0.1 mg
EUR 577.2

CD11b Activation Epitope Antibody (PE)

abx140359-100tests 100 tests
EUR 577.2

CD3 activation epitope Antibody (FITC)

abx140488-01mg 0.1 mg
EUR 510

anti-Lymphocyte Activation Gene 3

YF-PA12911 100 ug
EUR 483.6
Description: Rabbit polyclonal to Lymphocyte Activation Gene 3

Lymphocyte Activation Gene 3 Antibody

DF13960 100ul
EUR 420

Lymphocyte Activation Gene 3 Antibody

DF14459 100ul
EUR 420

Anti-GAL4 Activation Domain antibody

STJ97427 200 µl
EUR 236.4
Description: Rabbit polyclonal to GAL4 Activation Domain.

Anti-Hu CD11b (activation epitope) PE

1P-172-T025 25 tests
EUR 168

Anti-Hu CD11b (activation epitope) PE

1P-172-T100 100 tests
EUR 288

Rat Calpain Activation-1 (CAPNA1) Elisa Kit

YLA1388RA-48T 48T
EUR 465

Rat Calpain Activation-1 (CAPNA1) Elisa Kit

YLA1388RA-96T 96T
EUR 600

Rat B Cell Activation Factor ELISA kit

E02B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Activation Factor ELISA kit

E02B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Activation Factor ELISA kit

E02B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Activation Factor ELISA kit

E07B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Activation Factor ELISA kit

E07B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Activation Factor ELISA kit

E07B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Activation Factor ELISA kit

E08B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Activation Factor ELISA kit

E08B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Activation Factor ELISA kit

E08B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-CD3 epsilon (activation epitope) PE

1P-599-C025 0.025 mg
EUR 168

Anti-CD3 epsilon (activation epitope) PE

1P-599-C100 0.1 mg
EUR 288

Non-T-cell Activation Linker Protein

20-abx260597
  • EUR 3433.20
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

20-abx173814
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

20-abx128336
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

GAL4 Activation Domain Polyclonal Antibody

ABP57231-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

GAL4 Activation Domain Polyclonal Antibody

ABP57231-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

GAL4 Activation Domain Polyclonal Antibody

ABP57231-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

GAL4 Activation Domain Polyclonal Antibody

EA201-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain. This antibody is tested and validated for WB, ELISA

GAL4 Activation Domain Polyclonal Antibody

EA201-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain. This antibody is tested and validated for WB, ELISA

GAL4 Activation Domain Polyclonal Antibody

ES8230-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain from . This antibody is tested and validated for WB, ELISA

GAL4 Activation Domain Polyclonal Antibody

ES8230-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain from . This antibody is tested and validated for WB, ELISA

FAP Antibody / Fibroblast activation protein

RQ4022 100 ug
EUR 356.15
Description: Fibroblast activation protein alpha is a homodimeric 170 kDa melanoma membrane-bound gelatinase, protein that in humans is encoded by the FAP gene. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

anti-Fibroblast activation protein, alpha

YF-PA11714 50 ug
EUR 435.6
Description: Mouse polyclonal to Fibroblast activation protein, alpha

anti-Fibroblast activation protein, alpha

YF-PA11715 100 ug
EUR 483.6
Description: Rabbit polyclonal to Fibroblast activation protein, alpha

Goat B Cell Activation Factor ELISA kit

E06B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Activation Factor ELISA kit

E06B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Activation Factor ELISA kit

E06B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-CD3 epsilon (activation epitope) APC

1A-599-C100 0.1 mg
EUR 288

B-Cell Activation Protein (CD83) Antibody

20-abx312632
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

B-Cell Activation Protein (CD83) Antibody

20-abx001657
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

B-Cell Activation Protein (CD83) Antibody

abx414545-01mg 0.1 mg
EUR 526.8

B-Cell Activation Protein (CD83) Antibody

20-abx134019
  • EUR 427.20
  • EUR 678.00
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

B-Cell Activation Protein (CD83) Antibody

abx117184-100ug 100 ug
EUR 560.4

B-Cell Activation Protein (CD83) Antibody

abx146654-100ug 100 ug
EUR 526.8

B-Cell Activation Protein (CD83) Antibody

abx146735-100ug 100 ug
EUR 526.8

B-Cell Activation Protein (CD83) Antibody

abx432482-200ul 200 ul
EUR 460.8

Trypsinogen Activation Peptide (TAP) Antibody

20-abx103074
  • EUR 477.60
  • EUR 159.60
  • EUR 1328.40
  • EUR 644.40
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Trypsinogen Activation Peptide (TAP) Antibody

20-abx174951
  • EUR 944.40
  • EUR 493.20
  • 1 mg
  • 200 ug

Human B Cell Activation Factor ELISA kit

E01B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Activation Factor ELISA kit

E01B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Activation Factor ELISA kit

E01B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Activation Factor ELISA kit

E03B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Activation Factor ELISA kit

E03B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Activation Factor ELISA kit

E03B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-CD3 epsilon (activation epitope) FITC

1F-599-C100 0.1 mg
EUR 244.8

Recombinant Neutral Sphingomyelinase Activation Associated Factor (NSMAF)

4-RPE042Hu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Neutral Sphingomyelinase Activation Associated Factor expressed in: E.coli

T and B cell activation antigen antibody

10R-6574 100 ug
EUR 230.4
Description: Rat monoclonal T and B cell activation antigen antibody

Rabbit B Cell Activation Factor ELISA kit

E04B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Activation Factor ELISA kit

E04B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Activation Factor ELISA kit

E04B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Activation Factor ELISA kit

E09B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Activation Factor ELISA kit

E09B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Activation Factor ELISA kit

E09B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-Hu CD11b (activation epitope) Purified

11-172-C025 0.025 mg
EUR 118.8

Anti-Hu CD11b (activation epitope) Purified

11-172-C100 0.1 mg
EUR 189.6

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx211362
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx109319
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx113548
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx323649
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx007220
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx102894
  • EUR 493.20
  • EUR 159.60
  • EUR 1378.80
  • EUR 678.00
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx102895
  • EUR 360.00
  • EUR 159.60
  • EUR 961.20
  • EUR 510.00
  • EUR 309.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx173393
  • EUR 393.60
  • EUR 978.00
  • EUR 510.00
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lymphocyte Activation Gene 3 (LAG3) Antibody

20-abx130069
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Lymphocyte Activation Gene 3 (LAG3) Antibody

abx033099-400ul 400 ul
EUR 627.6

Lymphocyte Activation Gene 3 (LAG3) Antibody

abx033099-80l 80 µl
EUR 343.2

Anti-Fibroblast Activation Protein Antibody

A00422 100uL
EUR 531.6
Description: Rabbit Polyclonal Fibroblast Activation Protein Antibody. Validated in WB and tested in Mouse.

Pig Trypsinogen activation peptide ELISA kit

E07T0500-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Trypsinogen activation peptide ELISA kit

E07T0500-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Trypsinogen activation peptide ELISA kit

E07T0500-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Trypsinogen activation peptide ELISA kit

E02T0500-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Trypsinogen activation peptide ELISA kit

E02T0500-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Trypsinogen activation peptide ELISA kit

E02T0500-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Trypsinogen activation peptide ELISA kit

E08T0500-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Trypsinogen activation peptide ELISA kit

E08T0500-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Trypsinogen activation peptide ELISA kit

E08T0500-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat trypsinogen activation peptide ELISA Kit

ELA-E0634r 96 Tests
EUR 1063.2

B-Cell Activation Protein (CD83) Antibody (PE)

abx134018-100Assays 100 Assays
EUR 594

B-Cell Activation Protein (CD83) Antibody (PE)

abx134018-50Assays 50 Assays
EUR 477.6

Goat Trypsinogen activation peptide ELISA kit

E06T0500-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Trypsinogen activation peptide ELISA kit

E06T0500-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Trypsinogen activation peptide ELISA kit

E06T0500-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Fibroblast Activation Protein alpha (Recombinant)

20-abx074010
  • EUR 994.80
  • EUR 493.20
  • EUR 644.40
  • 10 ug
  • 2 µg
  • 5 ug

Human Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Protein

20-abx166616
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

B-Cell Activation Protein (CD83) Antibody (HRP)

20-abx312633
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

B-Cell Activation Protein (CD83) Antibody (RPE)

abx414546-100tests 100 tests
EUR 710.4

T-cell Activation Antigen CD27 (CD27) Protein

20-abx260944
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Anti-CD3 epsilon (activation epitope) Purified

11-599-C025 0.025 mg
EUR 129.6

Anti-CD3 epsilon (activation epitope) Purified

11-599-C100 0.1 mg
EUR 212.4

TNF-Related Activation Protein (TRAP) Antibody

abx032862-400ul 400 ul
EUR 627.6

TNF-Related Activation Protein (TRAP) Antibody

abx032862-80l 80 µl
EUR 343.2

TNF-Related Activation Protein (TRAP) Antibody

abx032863-400ul 400 ul
EUR 627.6

TNF-Related Activation Protein (TRAP) Antibody

abx032863-80l 80 µl
EUR 343.2

Human Trypsinogen activation peptide ELISA kit

E01T0500-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trypsinogen activation peptide ELISA kit

E01T0500-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Trypsinogen activation peptide ELISA kit

E01T0500-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Trypsinogen activation peptide ELISA kit

E03T0500-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Trypsinogen activation peptide ELISA kit

E03T0500-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Trypsinogen activation peptide ELISA kit

E03T0500-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human trypsinogen activation peptide ELISA Kit

ELA-E0634h 96 Tests
EUR 988.8

Recombinant Lymphocyte Activation Gene 3 (LAG3)

4-RPB663Hu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Lymphocyte Activation Gene 3 expressed in: E.coli

Recombinant Lymphocyte Activation Gene 3 (LAG3)

4-RPB663Mu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Lymphocyte Activation Gene 3 expressed in: E.coli

Recombinant Lymphocyte Activation Gene 3 (LAG3)

4-RPB663Ra01
  • EUR 612.44
  • EUR 286.80
  • EUR 1966.66
  • EUR 735.55
  • EUR 1351.10
  • EUR 484.80
  • EUR 4736.64
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Lymphocyte Activation Gene 3 expressed in: E.coli

Anti-Fibroblast activation protein, alpha (1E5)

YF-MA10315 100 ug
EUR 435.6
Description: Mouse monoclonal to Fibroblast activation protein, alpha

Anti-Fibroblast activation protein, alpha (2F2)

YF-MA12956 100 ug
EUR 435.6
Description: Mouse monoclonal to Fibroblast activation protein, alpha

Anti-Fibroblast activation protein, alpha (3C7)

YF-MA12957 100 ug
EUR 435.6
Description: Mouse monoclonal to Fibroblast activation protein, alpha

Dog Alternative macrophage activation associated CC chemokine 1 ELISA kit

E08A0790-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Alternative macrophage activation associated CC chemokine 1 ELISA kit

E08A0790-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Alternative macrophage activation associated CC chemokine 1 ELISA kit

E08A0790-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Alternative macrophage activation associated CC chemokine 1 ELISA kit

E07A0790-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Alternative macrophage activation associated CC chemokine 1 ELISA kit

E07A0790-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Alternative macrophage activation associated CC chemokine 1 ELISA kit

E07A0790-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Alternative macrophage activation associated CC chemokine 1 ELISA kit

E02A0790-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Alternative macrophage activation associated CC chemokine 1 ELISA kit

E02A0790-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Alternative macrophage activation associated CC chemokine 1 ELISA kit

E02A0790-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Linker For Activation Of T-Cell (LAT) Protein

20-abx262920
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

T Cell Activation RhoGTPase Activating Protein (TAGAP) Antibody

20-abx323101
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

T Cell Activation RhoGTPase Activating Protein (TAGAP) Antibody

20-abx301495
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

T-cell Activation Antigen CD27 (CD27) Antibody

20-abx211217
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

T-cell Activation Antigen CD27 (CD27) Antibody

20-abx225088
  • EUR 444.00
  • EUR 727.20
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 50 ul

T-cell Activation Antigen CD27 (CD27) Antibody

abx231453-100ug 100 ug
EUR 577.2

B-Cell Activation Protein (CD83) Antibody (FITC)

20-abx312634
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

T-cell Activation Antigen CD27 (CD27) Antibody

20-abx001588
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

B-Cell Activation Protein (CD83) Antibody (FITC)

abx414544-01mg 0.1 mg
EUR 610.8

T-cell Activation Antigen CD27 (CD27) Antibody

abx414870-025mg 0.25 mg
EUR 678

T-cell Activation Antigen CD27 (CD27) Antibody

abx415298-01mg 0.1 mg
EUR 543.6

T-cell Activation Antigen CD27 (CD27) Antibody

abx415489-01mg 0.1 mg
EUR 526.8

T-cell Activation Antigen CD27 (CD27) Antibody

abx415492-1mg 1 mg
EUR 994.8

T-cell Activation Antigen CD27 (CD27) Antibody

20-abx111471
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Rat Lymphocyte Activation Gene 3 (LAG3) Protein

20-abx167681
  • EUR 861.60
  • EUR 343.20
  • EUR 2648.40
  • EUR 1011.60
  • EUR 610.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

T-cell Activation Antigen CD27 (CD27) Antibody

20-abx211067
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

T-cell Activation Antigen CD27 (CD27) Antibody

abx139209-01mg 0.1 mg
EUR 427.2

Non-T-Cell Activation Linker (NTAL) Antibody

abx139940-01mg 0.1 mg
EUR 510

Non-T-Cell Activation Linker (NTAL) Antibody

abx139941-01mg 0.1 mg
EUR 510

Non-T-Cell Activation Linker (NTAL) Antibody

abx140049-01mg 0.1 mg
EUR 510

Non-T-Cell Activation Linker (NTAL) Antibody

abx140050-01mg 0.1 mg
EUR 510

Non-T-Cell Activation Linker (NTAL) Antibody

abx140051-01mg 0.1 mg
EUR 510

B-Cell Activation Protein (CD83) Antibody (FITC)

abx134017-100Assays 100 Assays
EUR 526.8

B-Cell Activation Protein (CD83) Antibody (FITC)

abx134017-50Assays 50 Assays
EUR 410.4

T-cell Activation Antigen CD27 (CD27) Antibody

abx146709-100ug 100 ug
EUR 526.8

T-cell Activation Antigen CD27 (CD27) Antibody

abx147085-100ug 100 ug
EUR 526.8

In vitro differentiatie van menselijke pluripotente stamcellen in de B-lijn met behulp van OP9-MS5 co-cultuur

 

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia.
Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).

Differentiatie van door de mens geïnduceerde pluripotente stamcellen tot definitief endoderm met behulp van een eenvoudig dialysecultuurapparaat

Therapeutic use of differentiated organ cells from human induced pluripotent stem cells (hiPSCs) is one of the promising strategies for regenerative medicine. Differentiation into definitive endoderm is an essential process in the preparation of metabolic organs. However, the manufacturability of differentiation is limited due to the high-cost cytokines required for the differentiation of endodermal lineage.
Furthermore, the cytokines remaining in the used culture medium and possible endogenous factors are removed along with toxic metabolites by the medium replacement. To address these problems, the application of dialysis culture can retain and fully utilize their accumulation to create a better culture environment that contributes to differentiation cost reduction.
The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0. Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic. We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.
In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and disialylated structures (SI:0.09) of which 75% of sialic acid was Neu5Gc. The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%). Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).
However, the effects on sialylation differed between the two Mabs. We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.
On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc. The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.
However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements. Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells. This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.

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