3D-cultuurmodellen als drugtestplatforms bij hondenlymfoom en hun overspraak met lymfeklier-afgeleide stromacellen
Background: Malignant lymphoma is the most common hematopoietic malignancy in dogs, and relapse is frequently seen despite aggressive initial treatment. In order for the treatment of these recurrent lymphomas in dogs to be effective, it is important to choose a personalized and sensitive anticancer agent. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key characteristics of the original tumor.
Objectives: In this study, we established a model of hybrid tumor/stromal spheroids and investigated the association between canine lymphoma cell line (GL-1) and canine lymph node (LN)-derived stromal cells (SCs).
Methods: A hybrid spheroid model consisting of GL-1 cells and LN-derived SC was created using ultra low attachment plate. The relationship between SCs and tumor cells (TCs) was investigated using a coculture system.
Results: TCs cocultured with SCs were found to have significantly upregulated multidrug resistance genes, such as P-qp, MRP1, and BCRP, compared with TC monocultures. Additionally, it was revealed that coculture with SCs reduced doxorubicin-induced apoptosis and G2/M cell cycle arrest of GL-1 cells.
Conclusions: SCs upregulated multidrug resistance genes in TCs and influenced apoptosis and the cell cycle of TCs in the presence of anticancer drugs. This study revealed that understanding the interaction between the tumor microenvironment and TCs is essential in designing experimental approaches to personalized medicine and to predict the effect of drugs.
Keywords: Canine lymphoma; drug resistance; lymph node-derived stromal cell; personalized cancer therapy; tumor microenvironment.
Het overwinnen van kweekbeperking voor SARS-CoV-2 in menselijke cellen vergemakkelijkt het screenen van verbindingen die virale replicatie remmen
Efforts to mitigate the COVID-19 pandemic include screening of existing antiviral molecules that could be re-purposed to treat SARS-CoV-2 infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nucs) often show decreased activity in these cells due to inefficient metabolization. SARS-CoV-2 exhibits low viability in human cells in culture.
Here, serial passages of a SARS-CoV-2 isolate (original-SARS2) in the human hepatoma cell clone Huh7.5 led to the selection of a variant (adapted-SARS2) with significantly improved infectivity in human liver (Huh7 and Huh7.5) and lung cancer cells (unmodified Calu-1 and A549). The adapted virus exhibited mutations in the spike protein, including a 9 amino acid deletion and 3 amino acid changes (E484D, P812R, and Q954H). E484D also emerged in Vero E6 cultured viruses that became viable in A549 cells.
Original and adapted viruses were susceptible to SR-B1 receptor blocking and adapted-SARS2 exhibited significantly less dependency of ACE2. Both variants were similarly neutralized by COVID-19 convalescent plasma but adapted-SARS2 exhibited increased susceptibility to exogenous type I interferon. Remdesivir inhibited original- and adapted-SARS2 similarly, demonstrating the utility of the system for the screening of nucs.
Among the tested nucs, only remdesivir, molnupiravir and to a limited extent galidesivir, showed antiviral effect across human cell lines, whereas sofosbuvir, ribavirin, and favipiravir had no apparent activity. Analogously to the emergence of spike mutations in vivo, the spike protein is under intense adaptive selection pressure in cell culture. Our results indicate that the emergence of spike mutations will most likely not affect the activity of remdesivir.
Rac1 Activation Assay |
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| STA-401-1 | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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Rac2 Activation Assay |
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| STA-401-2 | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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RhoA Activation Assay |
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| STA-403-A | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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RhoB Activation Assay |
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| STA-403-B | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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RhoC Activation Assay |
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| STA-403-C | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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Arf1 Activation Assay |
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| STA-407-1 | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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Arf6 Activation Assay |
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| STA-407-6 | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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Cdc42 Activation Assay |
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| STA-402 | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit. |
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Rap1 Activation Assay Kit |
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| STA-406-1 | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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Rap2 Activation Assay Kit |
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| STA-406-2 | Cell Biolabs | 20 assays | EUR 908.4 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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Ral Activation Assay Kit, Trial Size |
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| STA-408-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit. |
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Ran Activation Assay Kit, Trial Size |
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| STA-409-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit. |
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Rac1 Activation Assay Kit, Trial Size |
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| STA-401-1-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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Rac2 Activation Assay Kit, Trial Size |
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| STA-401-2-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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RhoA Activation Assay Kit, Trial Size |
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| STA-403-A-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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RhoB Activation Assay Kit, Trial Size |
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| STA-403-B-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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RhoC Activation Assay Kit, Trial Size |
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| STA-403-C-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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Rap1 Activation Assay Kit, Trial Size |
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| STA-406-1-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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Rap2 Activation Assay Kit, Trial Size |
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| STA-406-2-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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Arf1 Activation Assay Kit, Trial Size |
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| STA-407-1-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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Arf6 Activation Assay Kit, Trial Size |
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| STA-407-6-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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Cdc42 Activation Assay Kit, Trial Size |
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| STA-402-T | Cell Biolabs | 5 assays | EUR 483.6 |
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Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit. |
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Rac1/Cdc42 Activation Assay Combo Kit |
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| STA-404 | Cell Biolabs | 2 x 20 assays | EUR 1305.6 |
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Description: Our Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit. |
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RhoA/Rac1/CDC42 Activation Assay Combo Kit |
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| STA-405 | Cell Biolabs | 3 x 10 assays | EUR 1305.6 |
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Description: Our RhoA/Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit. |
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96-Well Ras Activation ELISA Kit (Colorimetric) |
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| STA-440 | Cell Biolabs | 96 assays | EUR 1082.4 |
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Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody. |
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96-Well Ras Activation ELISA Kit (Chemiluminescent) |
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| STA-441 | Cell Biolabs | 96 assays | EUR 1082.4 |
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Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody. |
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CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
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| 354730 | CORNING | 5/pk | EUR 102 |
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Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
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CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-MOUSE CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
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| 354720 | CORNING | 5/pk | EUR 576 |
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Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
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CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-HUMAN CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
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| 354725 | CORNING | 5/pk | EUR 568.8 |
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Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
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Stat3 Activation Inhibitor, SPI |
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| 1813-2 | Biovision | each | EUR 274.8 |
CD3 (activation epitope) Antibody |
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| abx139107-01mg | Abbexa | 0.1 mg | EUR 460.8 |
GAL4 Activation Domain Antibody |
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| 20-abx330273 | Abbexa |
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NF-?B Activation Inhibitor III |
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| C3559-25 | ApexBio | 25 mg | EUR 548.4 |
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Description: NF-?B Activation Inhibitor III is a NF-?B inhibitor.NF-?B (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex controling transcription of DNA, cytokine production as well as cell survival. |
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NF-?B Activation Inhibitor III |
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| C3559-5 | ApexBio | 5 mg | EUR 174 |
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Description: NF-?B Activation Inhibitor III is a NF-?B inhibitor.NF-?B (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex controling transcription of DNA, cytokine production as well as cell survival. |
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CD11b activation epitope Antibody |
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| abx140366-01mg | Abbexa | 0.1 mg | EUR 427.2 |
CD11b activation epitope Antibody |
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| abx140396-01mg | Abbexa | 0.1 mg | EUR 427.2 |
CD3 (activation epitope) Antibody (PE) |
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| abx139119-01mg | Abbexa | 0.1 mg | EUR 577.2 |
EZAct? Procathepsin Activation Buffer |
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| 1030-1 | Biovision | each | EUR 183.6 |
CD3 activation epitope Antibody (APC) |
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| abx140478-01mg | Abbexa | 0.1 mg | EUR 577.2 |
CD11b Activation Epitope Antibody (PE) |
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| abx140359-100tests | Abbexa | 100 tests | EUR 577.2 |
CD3 activation epitope Antibody (FITC) |
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| abx140488-01mg | Abbexa | 0.1 mg | EUR 510 |
anti-Lymphocyte Activation Gene 3 |
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| YF-PA12911 | Abfrontier | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal to Lymphocyte Activation Gene 3 |
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Lymphocyte Activation Gene 3 Antibody |
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| DF13960 | Affbiotech | 100ul | EUR 420 |
Lymphocyte Activation Gene 3 Antibody |
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| DF14459 | Affbiotech | 100ul | EUR 420 |
Anti-GAL4 Activation Domain antibody |
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| STJ97427 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to GAL4 Activation Domain. |
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Anti-Hu CD11b (activation epitope) PE |
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| 1P-172-T025 | ExBio | 25 tests | EUR 168 |
Anti-Hu CD11b (activation epitope) PE |
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| 1P-172-T100 | ExBio | 100 tests | EUR 288 |
Rat Calpain Activation-1 (CAPNA1) Elisa Kit |
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| YLA1388RA-48T | Shanghai YL Biotech | 48T | EUR 465 |
Rat Calpain Activation-1 (CAPNA1) Elisa Kit |
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| YLA1388RA-96T | Shanghai YL Biotech | 96T | EUR 600 |
Rat B Cell Activation Factor ELISA kit |
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| E02B0309-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat B Cell Activation Factor ELISA kit |
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| E02B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat B Cell Activation Factor ELISA kit |
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| E02B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig B Cell Activation Factor ELISA kit |
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| E07B0309-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig B Cell Activation Factor ELISA kit |
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| E07B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig B Cell Activation Factor ELISA kit |
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| E07B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog B Cell Activation Factor ELISA kit |
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| E08B0309-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog B Cell Activation Factor ELISA kit |
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| E08B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog B Cell Activation Factor ELISA kit |
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| E08B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Anti-CD3 epsilon (activation epitope) PE |
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| 1P-599-C025 | ExBio | 0.025 mg | EUR 168 |
Anti-CD3 epsilon (activation epitope) PE |
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| 1P-599-C100 | ExBio | 0.1 mg | EUR 288 |
Non-T-cell Activation Linker Protein |
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| 20-abx260597 | Abbexa |
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Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody |
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| 20-abx173814 | Abbexa |
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Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody |
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| 20-abx128336 | Abbexa |
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GAL4 Activation Domain Polyclonal Antibody |
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| ABP57231-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein |
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GAL4 Activation Domain Polyclonal Antibody |
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| ABP57231-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein |
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GAL4 Activation Domain Polyclonal Antibody |
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| ABP57231-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein |
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GAL4 Activation Domain Polyclonal Antibody |
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| EA201-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain. This antibody is tested and validated for WB, ELISA |
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GAL4 Activation Domain Polyclonal Antibody |
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| EA201-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain. This antibody is tested and validated for WB, ELISA |
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GAL4 Activation Domain Polyclonal Antibody |
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| ES8230-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain from . This antibody is tested and validated for WB, ELISA |
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GAL4 Activation Domain Polyclonal Antibody |
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| ES8230-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against GAL4 Activation Domain from . This antibody is tested and validated for WB, ELISA |
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FAP Antibody / Fibroblast activation protein |
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| RQ4022 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: Fibroblast activation protein alpha is a homodimeric 170 kDa melanoma membrane-bound gelatinase, protein that in humans is encoded by the FAP gene. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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anti-Fibroblast activation protein, alpha |
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| YF-PA11714 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to Fibroblast activation protein, alpha |
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anti-Fibroblast activation protein, alpha |
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| YF-PA11715 | Abfrontier | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal to Fibroblast activation protein, alpha |
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Goat B Cell Activation Factor ELISA kit |
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| E06B0309-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Goat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat B Cell Activation Factor ELISA kit |
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| E06B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Goat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat B Cell Activation Factor ELISA kit |
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| E06B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Goat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Anti-CD3 epsilon (activation epitope) APC |
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| 1A-599-C100 | ExBio | 0.1 mg | EUR 288 |
B-Cell Activation Protein (CD83) Antibody |
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| 20-abx312632 | Abbexa |
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B-Cell Activation Protein (CD83) Antibody |
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| 20-abx001657 | Abbexa |
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B-Cell Activation Protein (CD83) Antibody |
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| abx414545-01mg | Abbexa | 0.1 mg | EUR 526.8 |
B-Cell Activation Protein (CD83) Antibody |
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| 20-abx134019 | Abbexa |
|
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B-Cell Activation Protein (CD83) Antibody |
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| abx117184-100ug | Abbexa | 100 ug | EUR 560.4 |
B-Cell Activation Protein (CD83) Antibody |
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| abx146654-100ug | Abbexa | 100 ug | EUR 526.8 |
B-Cell Activation Protein (CD83) Antibody |
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| abx146735-100ug | Abbexa | 100 ug | EUR 526.8 |
B-Cell Activation Protein (CD83) Antibody |
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| abx432482-200ul | Abbexa | 200 ul | EUR 460.8 |
Trypsinogen Activation Peptide (TAP) Antibody |
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| 20-abx103074 | Abbexa |
|
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Trypsinogen Activation Peptide (TAP) Antibody |
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| 20-abx174951 | Abbexa |
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Human B Cell Activation Factor ELISA kit |
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| E01B0309-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Human B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human B Cell Activation Factor ELISA kit |
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| E01B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Human B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human B Cell Activation Factor ELISA kit |
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| E01B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Human B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse B Cell Activation Factor ELISA kit |
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| E03B0309-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse B Cell Activation Factor ELISA kit |
|||
| E03B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse B Cell Activation Factor ELISA kit |
|||
| E03B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Anti-CD3 epsilon (activation epitope) FITC |
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| 1F-599-C100 | ExBio | 0.1 mg | EUR 244.8 |
Recombinant Neutral Sphingomyelinase Activation Associated Factor (NSMAF) |
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| 4-RPE042Hu01 | Cloud-Clone |
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|
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Description: Recombinant Human Neutral Sphingomyelinase Activation Associated Factor expressed in: E.coli |
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T and B cell activation antigen antibody |
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| 10R-6574 | Fitzgerald | 100 ug | EUR 230.4 |
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Description: Rat monoclonal T and B cell activation antigen antibody |
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Rabbit B Cell Activation Factor ELISA kit |
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| E04B0309-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit B Cell Activation Factor ELISA kit |
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| E04B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit B Cell Activation Factor ELISA kit |
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| E04B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey B Cell Activation Factor ELISA kit |
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| E09B0309-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Monkey B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey B Cell Activation Factor ELISA kit |
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| E09B0309-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Monkey B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey B Cell Activation Factor ELISA kit |
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| E09B0309-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Monkey B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Anti-Hu CD11b (activation epitope) Purified |
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| 11-172-C025 | ExBio | 0.025 mg | EUR 118.8 |
Anti-Hu CD11b (activation epitope) Purified |
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| 11-172-C100 | ExBio | 0.1 mg | EUR 189.6 |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx211362 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx109319 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx113548 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx323649 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx007220 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx102894 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx102895 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx173393 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx130069 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx033099-400ul | Abbexa | 400 ul | EUR 627.6 |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx033099-80l | Abbexa | 80 µl | EUR 343.2 |
Anti-Fibroblast Activation Protein Antibody |
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| A00422 | BosterBio | 100uL | EUR 531.6 |
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Description: Rabbit Polyclonal Fibroblast Activation Protein Antibody. Validated in WB and tested in Mouse. |
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Pig Trypsinogen activation peptide ELISA kit |
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| E07T0500-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Porcine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Trypsinogen activation peptide ELISA kit |
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| E07T0500-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Porcine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Trypsinogen activation peptide ELISA kit |
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| E07T0500-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Porcine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Trypsinogen activation peptide ELISA kit |
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| E02T0500-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Rat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Trypsinogen activation peptide ELISA kit |
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| E02T0500-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Trypsinogen activation peptide ELISA kit |
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| E02T0500-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Trypsinogen activation peptide ELISA kit |
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| E08T0500-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Canine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Trypsinogen activation peptide ELISA kit |
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| E08T0500-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Canine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Trypsinogen activation peptide ELISA kit |
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| E08T0500-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat trypsinogen activation peptide ELISA Kit |
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| ELA-E0634r | Lifescience Market | 96 Tests | EUR 1063.2 |
B-Cell Activation Protein (CD83) Antibody (PE) |
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| abx134018-100Assays | Abbexa | 100 Assays | EUR 594 |
B-Cell Activation Protein (CD83) Antibody (PE) |
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| abx134018-50Assays | Abbexa | 50 Assays | EUR 477.6 |
Goat Trypsinogen activation peptide ELISA kit |
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| E06T0500-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Goat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Trypsinogen activation peptide ELISA kit |
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| E06T0500-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Goat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Trypsinogen activation peptide ELISA kit |
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| E06T0500-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Goat Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Fibroblast Activation Protein alpha (Recombinant) |
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| 20-abx074010 | Abbexa |
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Human Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Protein |
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| 20-abx166616 | Abbexa |
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B-Cell Activation Protein (CD83) Antibody (HRP) |
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| 20-abx312633 | Abbexa |
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B-Cell Activation Protein (CD83) Antibody (RPE) |
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| abx414546-100tests | Abbexa | 100 tests | EUR 710.4 |
T-cell Activation Antigen CD27 (CD27) Protein |
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| 20-abx260944 | Abbexa |
|
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Anti-CD3 epsilon (activation epitope) Purified |
|||
| 11-599-C025 | ExBio | 0.025 mg | EUR 129.6 |
Anti-CD3 epsilon (activation epitope) Purified |
|||
| 11-599-C100 | ExBio | 0.1 mg | EUR 212.4 |
TNF-Related Activation Protein (TRAP) Antibody |
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| abx032862-400ul | Abbexa | 400 ul | EUR 627.6 |
TNF-Related Activation Protein (TRAP) Antibody |
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| abx032862-80l | Abbexa | 80 µl | EUR 343.2 |
TNF-Related Activation Protein (TRAP) Antibody |
|||
| abx032863-400ul | Abbexa | 400 ul | EUR 627.6 |
TNF-Related Activation Protein (TRAP) Antibody |
|||
| abx032863-80l | Abbexa | 80 µl | EUR 343.2 |
Human Trypsinogen activation peptide ELISA kit |
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| E01T0500-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Human Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Trypsinogen activation peptide ELISA kit |
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| E01T0500-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Human Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Trypsinogen activation peptide ELISA kit |
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| E01T0500-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Human Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Trypsinogen activation peptide ELISA kit |
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| E03T0500-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Trypsinogen activation peptide ELISA kit |
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| E03T0500-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Mouse Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Trypsinogen activation peptide ELISA kit |
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| E03T0500-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Mouse Trypsinogen activation peptide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human trypsinogen activation peptide ELISA Kit |
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| ELA-E0634h | Lifescience Market | 96 Tests | EUR 988.8 |
Recombinant Lymphocyte Activation Gene 3 (LAG3) |
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| 4-RPB663Hu01 | Cloud-Clone |
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|
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Description: Recombinant Human Lymphocyte Activation Gene 3 expressed in: E.coli |
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Recombinant Lymphocyte Activation Gene 3 (LAG3) |
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| 4-RPB663Mu01 | Cloud-Clone |
|
|
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Description: Recombinant Mouse Lymphocyte Activation Gene 3 expressed in: E.coli |
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Recombinant Lymphocyte Activation Gene 3 (LAG3) |
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| 4-RPB663Ra01 | Cloud-Clone |
|
|
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Description: Recombinant Rat Lymphocyte Activation Gene 3 expressed in: E.coli |
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Anti-Fibroblast activation protein, alpha (1E5) |
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| YF-MA10315 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to Fibroblast activation protein, alpha |
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Anti-Fibroblast activation protein, alpha (2F2) |
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| YF-MA12956 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to Fibroblast activation protein, alpha |
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Anti-Fibroblast activation protein, alpha (3C7) |
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| YF-MA12957 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to Fibroblast activation protein, alpha |
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Dog Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E08A0790-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Canine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E08A0790-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Canine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E08A0790-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E07A0790-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Porcine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E07A0790-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Porcine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E07A0790-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Porcine Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E02A0790-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E02A0790-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Alternative macrophage activation associated CC chemokine 1 ELISA kit |
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| E02A0790-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Alternative macrophage activation associated CC chemokine 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Linker For Activation Of T-Cell (LAT) Protein |
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| 20-abx262920 | Abbexa |
|
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T Cell Activation RhoGTPase Activating Protein (TAGAP) Antibody |
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| 20-abx323101 | Abbexa |
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T Cell Activation RhoGTPase Activating Protein (TAGAP) Antibody |
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| 20-abx301495 | Abbexa |
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T-cell Activation Antigen CD27 (CD27) Antibody |
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| 20-abx211217 | Abbexa |
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T-cell Activation Antigen CD27 (CD27) Antibody |
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| 20-abx225088 | Abbexa |
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T-cell Activation Antigen CD27 (CD27) Antibody |
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| abx231453-100ug | Abbexa | 100 ug | EUR 577.2 |
B-Cell Activation Protein (CD83) Antibody (FITC) |
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| 20-abx312634 | Abbexa |
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T-cell Activation Antigen CD27 (CD27) Antibody |
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| 20-abx001588 | Abbexa |
|
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B-Cell Activation Protein (CD83) Antibody (FITC) |
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| abx414544-01mg | Abbexa | 0.1 mg | EUR 610.8 |
T-cell Activation Antigen CD27 (CD27) Antibody |
|||
| abx414870-025mg | Abbexa | 0.25 mg | EUR 678 |
T-cell Activation Antigen CD27 (CD27) Antibody |
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| abx415298-01mg | Abbexa | 0.1 mg | EUR 543.6 |
T-cell Activation Antigen CD27 (CD27) Antibody |
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| abx415489-01mg | Abbexa | 0.1 mg | EUR 526.8 |
T-cell Activation Antigen CD27 (CD27) Antibody |
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| abx415492-1mg | Abbexa | 1 mg | EUR 994.8 |
T-cell Activation Antigen CD27 (CD27) Antibody |
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| 20-abx111471 | Abbexa |
|
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Rat Lymphocyte Activation Gene 3 (LAG3) Protein |
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| 20-abx167681 | Abbexa |
|
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T-cell Activation Antigen CD27 (CD27) Antibody |
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| 20-abx211067 | Abbexa |
|
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T-cell Activation Antigen CD27 (CD27) Antibody |
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| abx139209-01mg | Abbexa | 0.1 mg | EUR 427.2 |
Non-T-Cell Activation Linker (NTAL) Antibody |
|||
| abx139940-01mg | Abbexa | 0.1 mg | EUR 510 |
Non-T-Cell Activation Linker (NTAL) Antibody |
|||
| abx139941-01mg | Abbexa | 0.1 mg | EUR 510 |
Non-T-Cell Activation Linker (NTAL) Antibody |
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| abx140049-01mg | Abbexa | 0.1 mg | EUR 510 |
Non-T-Cell Activation Linker (NTAL) Antibody |
|||
| abx140050-01mg | Abbexa | 0.1 mg | EUR 510 |
Non-T-Cell Activation Linker (NTAL) Antibody |
|||
| abx140051-01mg | Abbexa | 0.1 mg | EUR 510 |
B-Cell Activation Protein (CD83) Antibody (FITC) |
|||
| abx134017-100Assays | Abbexa | 100 Assays | EUR 526.8 |
B-Cell Activation Protein (CD83) Antibody (FITC) |
|||
| abx134017-50Assays | Abbexa | 50 Assays | EUR 410.4 |
T-cell Activation Antigen CD27 (CD27) Antibody |
|||
| abx146709-100ug | Abbexa | 100 ug | EUR 526.8 |
T-cell Activation Antigen CD27 (CD27) Antibody |
|||
| abx147085-100ug | Abbexa | 100 ug | EUR 526.8 |
In vitro differentiatie van menselijke pluripotente stamcellen in de B-lijn met behulp van OP9-MS5 co-cultuur
In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia.
Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).
Differentiatie van door de mens geïnduceerde pluripotente stamcellen tot definitief endoderm met behulp van een eenvoudig dialysecultuurapparaat
Therapeutic use of differentiated organ cells from human induced pluripotent stem cells (hiPSCs) is one of the promising strategies for regenerative medicine. Differentiation into definitive endoderm is an essential process in the preparation of metabolic organs. However, the manufacturability of differentiation is limited due to the high-cost cytokines required for the differentiation of endodermal lineage.
Furthermore, the cytokines remaining in the used culture medium and possible endogenous factors are removed along with toxic metabolites by the medium replacement. To address these problems, the application of dialysis culture can retain and fully utilize their accumulation to create a better culture environment that contributes to differentiation cost reduction.
The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0. Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic. We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.
In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and disialylated structures (SI:0.09) of which 75% of sialic acid was Neu5Gc. The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%). Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).
However, the effects on sialylation differed between the two Mabs. We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.
On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc. The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.
However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements. Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells. This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.