fondationvocation

Differentiatie van door de mens geïnduceerde pluripotente stamcellen

3D-cultuurmodellen als drugtestplatforms bij hondenlymfoom en hun overspraak met lymfeklier-afgeleide stromacellen

Background: Malignant lymphoma is the most common hematopoietic malignancy in dogs, and relapse is frequently seen despite aggressive initial treatment. In order for the treatment of these recurrent lymphomas in dogs to be effective, it is important to choose a personalized and sensitive anticancer agent. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key characteristics of the original tumor.
Objectives: In this study, we established a model of hybrid tumor/stromal spheroids and investigated the association between canine lymphoma cell line (GL-1) and canine lymph node (LN)-derived stromal cells (SCs).
Methods: A hybrid spheroid model consisting of GL-1 cells and LN-derived SC was created using ultra low attachment plate. The relationship between SCs and tumor cells (TCs) was investigated using a coculture system.
Results: TCs cocultured with SCs were found to have significantly upregulated multidrug resistance genes, such as P-qpMRP1, and BCRP, compared with TC monocultures. Additionally, it was revealed that coculture with SCs reduced doxorubicin-induced apoptosis and G2/M cell cycle arrest of GL-1 cells.
Conclusions: SCs upregulated multidrug resistance genes in TCs and influenced apoptosis and the cell cycle of TCs in the presence of anticancer drugs. This study revealed that understanding the interaction between the tumor microenvironment and TCs is essential in designing experimental approaches to personalized medicine and to predict the effect of drugs.
Keywords: Canine lymphoma; drug resistance; lymph node-derived stromal cell; personalized cancer therapy; tumor microenvironment.

Het overwinnen van kweekbeperking voor SARS-CoV-2 in menselijke cellen vergemakkelijkt het screenen van verbindingen die virale replicatie remmen

Efforts to mitigate the COVID-19 pandemic include screening of existing antiviral molecules that could be re-purposed to treat SARS-CoV-2 infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nucs) often show decreased activity in these cells due to inefficient metabolization. SARS-CoV-2 exhibits low viability in human cells in culture.
Here, serial passages of a SARS-CoV-2 isolate (original-SARS2) in the human hepatoma cell clone Huh7.5 led to the selection of a variant (adapted-SARS2) with significantly improved infectivity in human liver (Huh7 and Huh7.5) and lung cancer cells (unmodified Calu-1 and A549). The adapted virus exhibited mutations in the spike protein, including a 9 amino acid deletion and 3 amino acid changes (E484D, P812R, and Q954H). E484D also emerged in Vero E6 cultured viruses that became viable in A549 cells.
Original and adapted viruses were susceptible to SR-B1 receptor blocking and adapted-SARS2 exhibited significantly less dependency of ACE2. Both variants were similarly neutralized by COVID-19 convalescent plasma but adapted-SARS2 exhibited increased susceptibility to exogenous type I interferon. Remdesivir inhibited original- and adapted-SARS2 similarly, demonstrating the utility of the system for the screening of nucs.
Among the tested nucs, only remdesivir, molnupiravir and to a limited extent galidesivir, showed antiviral effect across human cell lines, whereas sofosbuvir, ribavirin, and favipiravir had no apparent activity. Analogously to the emergence of spike mutations in vivo, the spike protein is under intense adaptive selection pressure in cell culture. Our results indicate that the emergence of spike mutations will most likely not affect the activity of remdesivir.
fondationvocation
fondationvocation

K-Ras Activation Assay Kit, Trial Size

STA-400-K-T 5 assays
EUR 483.6
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

N-Ras Activation Assay Kit, Trial Size

STA-400-N-T 5 assays
EUR 483.6
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

Ral Activation Assay

STA-408 20 assays
EUR 908.4
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay

STA-409 20 assays
EUR 908.4
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

Rac1 Activation Assay

STA-401-1 20 assays
EUR 665

Rac2 Activation Assay

STA-401-2 20 assays
EUR 665

RhoA Activation Assay

STA-403-A 20 assays
EUR 665

RhoB Activation Assay

STA-403-B 20 assays
EUR 908.4
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay

STA-403-C 20 assays
EUR 908.4
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Arf1 Activation Assay

STA-407-1 20 assays
EUR 665

Arf6 Activation Assay

STA-407-6 20 assays
EUR 908.4
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Cdc42 Activation Assay

STA-402 20 assays
EUR 665

Rap1 Activation Assay Kit

STA-406-1 20 assays
EUR 665

Rap2 Activation Assay Kit

STA-406-2 20 assays
EUR 908.4
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

RalA Activation Assay Biochem Kit

BK040 KIT 50 assays Ask for price

Arf1 Pulldown Activation Assay Kit

BK032-S KIT 20 assays
EUR 540.8

Arf6 Pulldown Activation Assay Kit

BK033-S KIT 20 assays
EUR 540.8

Rac1 Pulldown Activation Assay Kit

BK035 KIT 50 assays
EUR 890.24

Rac1 Pulldown Activation Assay Kit

BK035-S KIT 20 assays
EUR 409.76

RhoA Pulldown Activation Assay Kit

BK036 KIT 80 assays
EUR 890.24

RhoA Pulldown Activation Assay Kit

BK036-S KIT 20 assays
EUR 409.76

Cdc42 Pulldown Activation Assay Kit

BK034 KIT 50 assays
EUR 890.24

Cdc42 Pulldown Activation Assay Kit

BK034-S KIT 20 assays
EUR 409.76

Rac1 G-LISA Activation Assay Kit

BK126 KIT 96 assays
EUR 1036.88

Ral Activation Assay Kit, Trial Size

STA-408-T 5 assays
EUR 483.6
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay Kit, Trial Size

STA-409-T 5 assays
EUR 483.6
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

Rac1 Activation Assay Kit, Trial Size

STA-401-1-T 5 assays
EUR 483.6
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rac2 Activation Assay Kit, Trial Size

STA-401-2-T 5 assays
EUR 483.6
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

RhoA Activation Assay Kit, Trial Size

STA-403-A-T 5 assays
EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoB Activation Assay Kit, Trial Size

STA-403-B-T 5 assays
EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay Kit, Trial Size

STA-403-C-T 5 assays
EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Rap1 Activation Assay Kit, Trial Size

STA-406-1-T 5 assays
EUR 483.6
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rap2 Activation Assay Kit, Trial Size

STA-406-2-T 5 assays
EUR 483.6
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Arf1 Activation Assay Kit, Trial Size

STA-407-1-T 5 assays
EUR 483.6
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Arf6 Activation Assay Kit, Trial Size

STA-407-6-T 5 assays
EUR 483.6
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Cdc42 Activation Assay Kit, Trial Size

STA-402-T 5 assays
EUR 483.6
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

Rac1/Cdc42 Activation Assay Combo Kit

STA-404 2 x 20 assays
EUR 975

RhoA G-LISA Activation Assay (luminescence)

BK121 KIT 96 assays
EUR 1036.88

RhoA G-LISA Activation Assay (colorimetric)

BK124 KIT 96 assays
EUR 1036.88

RhoA G-LISA Activation Assay (colorimetric)

BK124-S KIT 24 assays
EUR 626.08

RhoA G-LISA Activation Assay (colorimetric)

BK124-XL Large Quantities Ask for price

Combo RhoA/Rac1/Cdc42 Activation Assay Kit

BK030 KIT 3 x 10 assays
EUR 1098.24

RhoA/Rac1/CDC42 Activation Assay Combo Kit

STA-405 3 x 10 assays
EUR 975

Rac1,2,3 G-LISA Activation Assay Kit (colorimetric)

BK125 KIT 96 assays
EUR 1036.88

Rac1 G-LISA Activation Assay Kit (colorimetric)

BK128 KIT 96 assays
EUR 1036.88

Rac1 G-LISA Activation Assay Kit (colorimetric)

BK128-S KIT 24 assays
EUR 626.08

RalA G-LISA Activation Assay Kit (colorimetric)

BK129 KIT 96 assays
EUR 1036.88

Cdc42 G-LISA Activation Assay Kit (colorimetric)

BK127 KIT 96 assays
EUR 1036.88

Cdc42 G-LISA Activation Assay Kit (colorimetric)

BK127-S KIT 24 assays
EUR 626.08

Trypsinogen Activation Peptide (TAP) Magnetic Luminex Assay Kit

LKU603513-96T 96T
EUR 944.2

Trypsinogen Activation Peptide (TAP) Magnetic Luminex Assay Kit

LKU603521-96T 96T
EUR 894.7

Trypsinogen Activation Peptide (TAP) Magnetic Luminex Assay Kit

LKU603531-96T 96T
EUR 869.4

Lymphocyte Activation Gene 3 (LAG3) Magnetic Luminex Assay Kit

LKU607300-96T 96T
EUR 917.7

Lymphocyte Activation Gene 3 (LAG3) Magnetic Luminex Assay Kit

LKU607304-96T 96T
EUR 944.2

Fibroblast Activation Protein Alpha (FAPa) Magnetic Luminex Assay Kit

LKU601220-96T 96T
EUR 993.6

Fibroblast Activation Protein Alpha (FAPa) Magnetic Luminex Assay Kit

LKU601221-96T 96T
EUR 966

Fibroblast Activation Protein Alpha (FAPa) Magnetic Luminex Assay Kit

LKU601222-96T 96T
EUR 1048.8

Linker For Activation Of T-Cell (LAT) Magnetic Luminex Assay Kit

LKU601732-96T 96T
EUR 869.4

Thymus Activation Regulated Chemokine (TARC) Magnetic Luminex Assay Kit

LKU603970-96T 96T
EUR 995.9

Thymus Activation Regulated Chemokine (TARC) Magnetic Luminex Assay Kit

LKU603977-96T 96T
EUR 944.2

Thymus Activation Regulated Chemokine (TARC) Magnetic Luminex Assay Kit

LKU603996-96T 96T
EUR 869.4

B-Cell Activation Factor Receptor (BAFFR) Magnetic Luminex Assay Kit

LKU603426-96T 96T
EUR 869.4

Activation Induced Cytidine Deaminase (AICDA) Magnetic Luminex Assay Kit

LKU606582-96T 96T
EUR 966

Pulmonary Activation Regulated Chemokine (PARC) Magnetic Luminex Assay Kit

LKU605817-96T 96T
EUR 770.5

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Magnetic Luminex Assay Kit

LKU603761-96T 96T
EUR 966

96-Well Ras Activation ELISA Kit (Colorimetric)

STA-440 96 assays
EUR 810

B-Lymphocyte Activation Antigen B7-1 (LAB7-1) Magnetic Luminex Assay Kit

LKU603336-96T 96T
EUR 917.7

96-Well Ras Activation ELISA Kit (Chemiluminescent)

STA-441 96 assays
EUR 810

T-Cell Activation Antigen, Increased Late Expression (TACTILE) Magnetic Luminex Assay Kit

LKU604679-96T 96T
EUR 966

Signaling Lymphocytic Activation Molecule Family, Member 2 (SLAMF2) Magnetic Luminex Assay Kit

LKU603532-96T 96T
EUR 966

Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) Magnetic Luminex Assay Kit

LKU603538-96T 96T
EUR 966

V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) Magnetic Luminex Assay Kit

LKU603615-96T 96T
EUR 966

Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Theta (YWHAq) Magnetic Luminex Assay Kit

LKU607555-96T 96T
EUR 993.6

Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) Magnetic Luminex Assay Kit

LKU607558-96T 96T
EUR 993.6

Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) Magnetic Luminex Assay Kit

LKU607552-96T 96T
EUR 966

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Magnetic Luminex Assay Kit

LKU600254-96T 96T
EUR 993.6

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Magnetic Luminex Assay Kit

LKU607693-96T 96T
EUR 770.5

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Magnetic Luminex Assay Kit

LKU607695-96T 96T
EUR 770.5

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Magnetic Luminex Assay Kit

LKU607696-96T 96T
EUR 770.5

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Magnetic Luminex Assay Kit

LKU607697-96T 96T
EUR 993.6

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Magnetic Luminex Assay Kit

LKU607698-96T 96T
EUR 1043.1

CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE

354730 5/pk
EUR 102
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-MOUSE CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE

354720 5/pk
EUR 576
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-HUMAN CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE

354725 5/pk
EUR 568.8
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

NFAT activation molecule 1

AP85978 1mg
EUR 2640

NFAT activation molecule 1

AP86130 1mg
EUR 2640

Early activation antigen CD69

AP81561 1mg
EUR 2640

Early activation antigen CD69

AP81836 1mg
EUR 2640

Stat3 Activation Inhibitor, SPI

1813-2 each
EUR 274.8

CD3 (activation epitope) Antibody

abx139107-01mg 0.1 mg
EUR 460.8

GAL4 Activation Domain Antibody

20-abx330273
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

CD3 (activation epitope) Antibody

abx139107-100l 100 µl
EUR 300

CD3 (activation epitope) Antibody

abx139107-500l 500 µl Ask for price

CD18 (Activation Epitope) Antibody

GWB-9C757A 0.02 mg Ask for price

CD18 (Activation Epitope) Antibody

GWB-778F3E 0.2 mg Ask for price

CD11b activation epitope Antibody

abx140366-01mg 0.1 mg
EUR 427.2

CD11b activation epitope Antibody

abx140396-01mg 0.1 mg
EUR 427.2

CD11b activation epitope Antibody

abx140366-100g 100 µg
EUR 275

CD11b activation epitope Antibody

abx140396-100g 100 µg
EUR 275

Trypsinogen Activation Peptide,TAP

AP79714 1mg
EUR 2640

Trypsinogen Activation Peptide,TAP

AP79935 1mg
EUR 2640

NF-κB Activation Inhibitor III

C3559-25 25 mg
EUR 325
Description: NF-κB inhibitor

NF-κB Activation Inhibitor III

C3559-5 5 mg
EUR 77
Description: NF-κB inhibitor

GAL4 Activation Domain Rabbit pAb

E2350167 100ul
EUR 225
Description: Available in various conjugation types.

EZAct? Procathepsin Activation Buffer

1030-1 each
EUR 183.6

CD3 (activation epitope) Antibody (PE)

abx139119-01mg 0.1 mg
EUR 577.2

CD3 (activation epitope) Antibody (PE)

abx139119-100l 100 µl
EUR 400

CD3 (activation epitope) Antibody (PE)

abx139119-500l 500 µl Ask for price

CD3 activation epitope Antibody (APC)

abx140478-01mg 0.1 mg
EUR 577.2

CD3 activation epitope Antibody (APC)

abx140478-100g 100 µg
EUR 400

CD11b Activation Epitope Antibody (PE)

abx140359-100tests 100 tests
EUR 577.2

CD3 activation epitope Antibody (FITC)

abx140488-01mg 0.1 mg
EUR 510

Cd18 (Activation Epitope) Antibody (RPE)

GWB-17EC2C 100 TESTS Ask for price

CD18 (Activation Epitope) Antibody (RPE)

GWB-Q01003 25 TESTS Ask for price

anti-Lymphocyte Activation Gene 3

YF-PA12911 100 ug
EUR 483.6
Description: Rabbit polyclonal to Lymphocyte Activation Gene 3

CD11b Activation Epitope Antibody (PE)

abx140359-100g 100 µg
EUR 400

CD3 activation epitope Antibody (FITC)

abx140488-100g 100 µg
EUR 350

Activation-induced cytidine deaminase

AP80386 1mg
EUR 2640

Lymphocyte activation gene 3 protein

AP84383 1mg
EUR 2640

Lymphocyte activation gene 3 protein

AP84823 1mg
EUR 2640

Lymphocyte activation gene 3 protein

AP85011 1mg
EUR 2640

Cd18 (Activation Epitope) Antibody (FITC)

GWB-550993 0.025 mg Ask for price

Cd18 (Activation Epitope) Antibody (FITC)

GWB-56BD51 0.1 mg Ask for price

Active Lymphocyte Activation Gene 3 (LAG3)

APB663Mu61 10ug
EUR 300

Active Lymphocyte Activation Gene 3 (LAG3)

APB663Ra61 10ug
EUR 239

Lymphocyte Activation Gene 3 Antibody

DF13960 100ul
EUR 420

Lymphocyte Activation Gene 3 Antibody

DF13960-100ul 100ul
EUR 280

Lymphocyte Activation Gene 3 Antibody

DF13960-200ul 200ul
EUR 350

Lymphocyte Activation Gene 3 Antibody

DF14459 100ul
EUR 420

Lymphocyte Activation Gene 3 Antibody

DF14459-100ul 100ul
EUR 280

Lymphocyte Activation Gene 3 Antibody

DF14459-200ul 200ul
EUR 350

T-lymphocyte activation antigen CD86

AP78680 1mg
EUR 2640

T-lymphocyte activation antigen CD80

AP79265 1mg
EUR 2640

T-lymphocyte activation antigen CD80

AP81755 1mg
EUR 2640

T-lymphocyte activation antigen CD86

AP81806 1mg
EUR 2640

Anti-GAL4 Activation Domain antibody

STJ97427 200 µl
EUR 236.4
Description: Rabbit polyclonal to GAL4 Activation Domain.

Anti-Hu CD11b (activation epitope) PE

1P-172-T025 25 tests
EUR 121

Anti-Hu CD11b (activation epitope) PE

1P-172-T100 100 tests
EUR 242

Rat Calpain Activation-1 (CAPNA1) Elisa Kit

YLA1388RA-48T 48T Ask for price

Rat Calpain Activation-1 (CAPNA1) Elisa Kit

YLA1388RA-96T 96T Ask for price

Thrombin Activation peptide fragment 1

E8ER1917-70 100ul
EUR 275
Description: Available in various conjugation types.

Cd18 (Activation Epitope) Antibody (Biotin)

GWB-AB34A9 0.1 mg Ask for price

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

20-abx128336
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  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

20-abx173814
  • Ask for price
  • Ask for price
  • 1 mg
  • 200 ug

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

abx128336-100l 100 µl
EUR 275

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

abx128336-1ml 1 ml
EUR 750

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

abx128336-200l 200 µl
EUR 337.5

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

abx173814-100l 100 µl
EUR 787.5

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

abx173814-1ml 1 ml Ask for price

Neutral Sphingomyelinase Activation Associated Factor (NSMAF) Antibody

abx173814-200l 200 µl Ask for price

AP-1/NF-κB activation inhibitor 1

T9656-10mg 10mg Ask for price
Description: AP-1/NF-κB activation inhibitor 1

AP-1/NF-κB activation inhibitor 1

T9656-1g 1g Ask for price
Description: AP-1/NF-κB activation inhibitor 1

AP-1/NF-κB activation inhibitor 1

T9656-1mg 1mg Ask for price
Description: AP-1/NF-κB activation inhibitor 1

AP-1/NF-κB activation inhibitor 1

T9656-50mg 50mg Ask for price
Description: AP-1/NF-κB activation inhibitor 1

AP-1/NF-κB activation inhibitor 1

T9656-5mg 5mg Ask for price
Description: AP-1/NF-κB activation inhibitor 1

Recombinant Neutral Sphingomyelinase Activation Associated Factor (NSMAF)

RPE042Hu01 10ug
EUR 164

Recombinant Neutral Sphingomyelinase Activation Associated Factor (NSMAF)

4-RPE042Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Neutral Sphingomyelinase Activation Associated Factor expressed in: E.coli

Recombinant Neutral Sphingomyelinase Activation Associated Factor (NSMAF)

RPU51861-100ug 100ug
EUR 504.9

Recombinant Neutral Sphingomyelinase Activation Associated Factor (NSMAF)

RPU51861-1mg 1mg
EUR 2238.6

Recombinant Neutral Sphingomyelinase Activation Associated Factor (NSMAF)

RPU51861-50ug 50ug
EUR 405.9

Anti-CD3 epsilon (activation epitope) PE

1P-599-C025 0.025 mg
EUR 121

Anti-CD3 epsilon (activation epitope) PE

1P-599-C100 0.1 mg
EUR 242

Non-T-cell Activation Linker Protein

20-abx260597
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  • 1 mg
  • 25 ug
  • 5 ug

Non-T-cell Activation Linker Protein

abx260597-200g 200 µg
EUR 325

Non-T-cell Activation Linker Protein

abx260597-50g 50 µg
EUR 225

Rat B Cell Activation Factor ELISA kit

E02B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Activation Factor ELISA kit

E02B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Activation Factor ELISA kit

E02B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Activation Factor ELISA kit

E07B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Activation Factor ELISA kit

E07B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Activation Factor ELISA kit

E07B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Activation Factor ELISA kit

E08B0309-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Activation Factor ELISA kit

E08B0309-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Activation Factor ELISA kit

E08B0309-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine B Cell Activation Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Activation Factor ELISA kit

E01A10465 96T
EUR 700
Description: ELISA

GAL4 Activation Domain Polyclonal Antibody

ABP57231-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

GAL4 Activation Domain Polyclonal Antibody

ABP57231-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

GAL4 Activation Domain Polyclonal Antibody

ABP57231-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GAL4 Activation Domain from Human. This GAL4 Activation Domain antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

GAL4 Activation Domain Polyclonal Antibody

ABP57231-100uL 100 μL
EUR 239
Description: Rabbit Anti-GAL4 Activation Domain Polyclonal Antibody

GAL4 Activation Domain Polyclonal Antibody

ABP57231-200uL 200 μL
EUR 379
Description: Rabbit Anti-GAL4 Activation Domain Polyclonal Antibody

GAL4 Activation Domain Polyclonal Antibody

ABP57231-30uL 30 μL
EUR 109
Description: Rabbit Anti-GAL4 Activation Domain Polyclonal Antibody

GAL4 Activation Domain Polyclonal Antibody

E20-53410 100ug
EUR 225
Description: Available in various conjugation types.

FAP Antibody / Fibroblast activation protein

RQ4022 100 ug
EUR 356.15
Description: Fibroblast activation protein alpha is a homodimeric 170 kDa melanoma membrane-bound gelatinase, protein that in humans is encoded by the FAP gene. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

anti-Fibroblast activation protein, alpha

YF-PA11714 50 ug
EUR 435.6
Description: Mouse polyclonal to Fibroblast activation protein, alpha

anti-Fibroblast activation protein, alpha

YF-PA11715 100 ug
EUR 483.6
Description: Rabbit polyclonal to Fibroblast activation protein, alpha

GAL4 Activation Domain Polyclonal Antibody

E44H11033 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

B-Cell Activation Protein (CD83) Antibody

abx117184-100ug 100 ug
EUR 560.4

B-Cell Activation Protein (CD83) Antibody

abx146654-100ug 100 ug
EUR 526.8

B-Cell Activation Protein (CD83) Antibody

abx146735-100ug 100 ug
EUR 526.8

B-Cell Activation Protein (CD83) Antibody

20-abx134019
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In vitro differentiatie van menselijke pluripotente stamcellen in de B-lijn met behulp van OP9-MS5 co-cultuur

 

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia.
Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).

Differentiatie van door de mens geïnduceerde pluripotente stamcellen tot definitief endoderm met behulp van een eenvoudig dialysecultuurapparaat

Therapeutic use of differentiated organ cells from human induced pluripotent stem cells (hiPSCs) is one of the promising strategies for regenerative medicine. Differentiation into definitive endoderm is an essential process in the preparation of metabolic organs. However, the manufacturability of differentiation is limited due to the high-cost cytokines required for the differentiation of endodermal lineage.
Furthermore, the cytokines remaining in the used culture medium and possible endogenous factors are removed along with toxic metabolites by the medium replacement. To address these problems, the application of dialysis culture can retain and fully utilize their accumulation to create a better culture environment that contributes to differentiation cost reduction.
The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0. Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic. We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.
In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and disialylated structures (SI:0.09) of which 75% of sialic acid was Neu5Gc. The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%). Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).
However, the effects on sialylation differed between the two Mabs. We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.
On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc. The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.
However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements. Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells. This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.

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